Comparison of solution structure of free and complexed lac operator by molecular modelling with NMR constraints

Changes inlac Operator dynamics upon selective interaction withlac Repressor as revealed by heteronuclear relaxation rate measurements

“…These data were similar to those already reported on two complexes of the N-terminal headpiece of lac Repressor, one with a nine base-pair duplex 11 and the other with a 14 base-pair duplex. 2 The specificity of interaction was verified by comparing the results obtained by complexing the N-terminal headpiece with the self-complementary decanucleotide d(AATTGCAATT) 2 , which differed from the lac Operator sequence by four base pairs.…”

“…The N-terminal headpiece 1-51 was prepared as described. 11 Complexed operator was prepared by mixing with an N-terminal headpiece solution. The mixture was dialysed against potassium phosphate solution (1 mM) at pH 6.8, then lyophilized from D 2 O and dissolved in D 2 O containing 7 mM phosphate.…”

Changes inlac Operator dynamics upon selective interaction withlac Repressor as revealed by heteronuclear relaxation rate measurements

“…These data were similar to those already reported on two complexes of the N-terminal headpiece of lac Repressor, one with a nine base-pair duplex 11 and the other with a 14 base-pair duplex. 2 The specificity of interaction was verified by comparing the results obtained by complexing the N-terminal headpiece with the self-complementary decanucleotide d(AATTGCAATT) 2 , which differed from the lac Operator sequence by four base pairs.…”

“…The N-terminal headpiece 1-51 was prepared as described. 11 Complexed operator was prepared by mixing with an N-terminal headpiece solution. The mixture was dialysed against potassium phosphate solution (1 mM) at pH 6.8, then lyophilized from D 2 O and dissolved in D 2 O containing 7 mM phosphate.…”

Solution structure of oligonucleotides covalently linked to a psoralen derivative

“…Based on a combination of internal variables and helical variables, this program permits important and efficient structural deformations during energy minimization and allows various types of controls. The method has already proved powerful for generating 3-D structures of oligonucleotides from NMR data (14)(15). In order to model the cross-linked psoralen, we first introduced a modified thymine species in the nucleic acids library associated with JUMNA.…”

The experimental folding landscape of monomeric lactose repressor, a large two-domain protein, involves two kinetic intermediates

“…2C). When LacI is not bound to DNA, the DNAbinding domain (residues 1-60) appears unstructured (29,37,38); therefore, secondary structure contribution from this domain is negligible.…”

Changes inlac Operator dynamics upon selective interaction withlac Repressor as revealed by heteronuclear relaxation rate measurements

“…These data were similar to those already reported on two complexes of the N-terminal headpiece of lac Repressor, one with a nine base-pair duplex 11 and the other with a 14 base-pair duplex. 2 The specificity of interaction was verified by comparing the results obtained by complexing the N-terminal headpiece with the self-complementary decanucleotide d(AATTGCAATT) 2 , which differed from the lac Operator sequence by four base pairs.…”

“…The N-terminal headpiece 1-51 was prepared as described. 11 Complexed operator was prepared by mixing with an N-terminal headpiece solution. The mixture was dialysed against potassium phosphate solution (1 mM) at pH 6.8, then lyophilized from D 2 O and dissolved in D 2 O containing 7 mM phosphate.…”

Solution structure of oligonucleotides covalently linked to a psoralen derivative

“…Based on a combination of internal variables and helical variables, this program permits important and efficient structural deformations during energy minimization and allows various types of controls. The method has already proved powerful for generating 3-D structures of oligonucleotides from NMR data (14)(15). In order to model the cross-linked psoralen, we first introduced a modified thymine species in the nucleic acids library associated with JUMNA.…”

The experimental folding landscape of monomeric lactose repressor, a large two-domain protein, involves two kinetic intermediates

“…2C). When LacI is not bound to DNA, the DNAbinding domain (residues 1-60) appears unstructured (29,37,38); therefore, secondary structure contribution from this domain is negligible.…”

Changes inlac Operator dynamics upon selective interaction withlac Repressor as revealed by heteronuclear relaxation rate measurements

“…These data were similar to those already reported on two complexes of the N-terminal headpiece of lac Repressor, one with a nine base-pair duplex 11 and the other with a 14 base-pair duplex. 2 The specificity of interaction was verified by comparing the results obtained by complexing the N-terminal headpiece with the self-complementary decanucleotide d(AATTGCAATT) 2 , which differed from the lac Operator sequence by four base pairs.…”

“…The N-terminal headpiece 1-51 was prepared as described. 11 Complexed operator was prepared by mixing with an N-terminal headpiece solution. The mixture was dialysed against potassium phosphate solution (1 mM) at pH 6.8, then lyophilized from D 2 O and dissolved in D 2 O containing 7 mM phosphate.…”

Solution structure of oligonucleotides covalently linked to a psoralen derivative

“…Based on a combination of internal variables and helical variables, this program permits important and efficient structural deformations during energy minimization and allows various types of controls. The method has already proved powerful for generating 3-D structures of oligonucleotides from NMR data (14)(15). In order to model the cross-linked psoralen, we first introduced a modified thymine species in the nucleic acids library associated with JUMNA.…”

The experimental folding landscape of monomeric lactose repressor, a large two-domain protein, involves two kinetic intermediates

“…2C). When LacI is not bound to DNA, the DNAbinding domain (residues 1-60) appears unstructured (29,37,38); therefore, secondary structure contribution from this domain is negligible.…”