2010
DOI: 10.1590/s1678-91992010000400004
View full text |Buy / Rent full text
|
Sign up to set email alerts
|

Abstract: Abstract:In recent years, the polymerase chain reaction (PCR) technique has significantly advanced towards expanding its use and versatility by working with quantitative real-time PCR (qPCR). Data from the literature show that both methods present interesting characteristics for the diagnosis of visceral leishmaniasis. The benefits of qPCR in relation to conventional PCR include speed, reproducibility and quantitative ability. In addition to operational advantages, qPCR is more sensitive and reproducible and m… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
2
1

Citation Types

0
30
0
1

Year Published

2012
2012
2017
2017

Publication Types

Select...
4
1

Relationship

1
4

Authors

Journals

citations
Cited by 34 publications
(37 citation statements)
references
References 28 publications
(37 reference statements)
0
30
0
1
Order By: Relevance
“…The operational advantages of real time PCR over conventional PCR are quantitative ability, speed, higher sensitivity and reproducibility [36].…”
Section: Discussionmentioning
confidence: 99%
“…The operational advantages of real time PCR over conventional PCR are quantitative ability, speed, higher sensitivity and reproducibility [36].…”
Section: Discussionmentioning
confidence: 99%
“…6,7 Of the four Leishmaniapositive samples, three had a previous negative PCR for Leishmania spp. performed before their inclusion in the study; this might be related to the lower sensitivity of the direct PCR method in comparison to RT-PCR 14,15,33,34 and/or to inappropriate selection of paraffin-embedded material or of the probes. Our results provide further support that RT-PCR, as previously documented, 14,15 is superior to immunohistochemistry in detecting Leishmania spp.…”
Section: Discussionmentioning
confidence: 99%
“…qPCR is considered to be a helpful tool for Leishmania diagnosis, monitoring during therapy, development of new drugs and diagnostic tools, comparison of drug efficacy or prophylactic schemes, and for epidemiological studies. Regarding diagnosis of leishmaniasis, the kinetic study of parasitemia in the immunocompromised hosts, the diagnosis of relapses and the quantification of the low parasitic load in asymptomatic patients are of great interest [47].…”
Section: Molecular Diagnosismentioning
confidence: 99%