Comparison of rat hepatic and pulmonary microsomal metabolism of benzene and the lack of benzene-induced pneumotoxicity and hepatotoxicity

Cytochromes P450 involved with benzene metabolism in hepatic and pulmonary microsomes

“…At 1000 lM benzene, CYP2E1, CYP2F, and CYP2B seemed to be equally involved in metabolism. These results are in close agreement with those of Chaney and Carlson [19], who showed that 17.5 lM benzene metabolism in both rat liver and lung was inhibited by DDTC (300 lM), indicating that CYP2E1 is major isozyme involved in benzene metabolism. In their study benzene metabolism in liver was inhibited by 96% and in lung by 54%, compared to 92% and 41%, respectively, in our current study.…”

“…CYP2E1 was the P450 isozyme most involved at both 24 lM and 1000 lM benzene in hepatic microsomes, consistent with the published results of other researchers [19,36,37]. CYP2F and CYP2E1 were both involved in 24 lM benzene metabolism by rat pulmonary microsomes.…”

Cytochromes P450 involved with benzene metabolism in hepatic and pulmonary microsomes

“…At 1000 lM benzene, CYP2E1, CYP2F, and CYP2B seemed to be equally involved in metabolism. These results are in close agreement with those of Chaney and Carlson [19], who showed that 17.5 lM benzene metabolism in both rat liver and lung was inhibited by DDTC (300 lM), indicating that CYP2E1 is major isozyme involved in benzene metabolism. In their study benzene metabolism in liver was inhibited by 96% and in lung by 54%, compared to 92% and 41%, respectively, in our current study.…”

“…CYP2E1 was the P450 isozyme most involved at both 24 lM and 1000 lM benzene in hepatic microsomes, consistent with the published results of other researchers [19,36,37]. CYP2F and CYP2E1 were both involved in 24 lM benzene metabolism by rat pulmonary microsomes.…”

Benzene metabolism in human lung cell lines BEAS‐2B and A549 and cells overexpressing CYP2F1

“…The components of the organic layer were analyzed using a Shimadzu HPLC system with a Beckman octadecyl silane (ODS) column ( (or per 10 6 cells)/45 min. This time period was in keeping with our previous studies on tissues [16][17][18][29][30][31][32]. Since the primary metabolite was phenol with only traces of hydroquinone and catechol, derived from further metabolism of this metabolite, being identified, the results are expressed as total hydroxylated metabolite.…”

The Role of Metabolism and Specific Metabolites in Benzene-Induced Toxicity: Evidence and Issues

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Cytochromes P450 involved with benzene metabolism in hepatic and pulmonary microsomes

“…At 1000 lM benzene, CYP2E1, CYP2F, and CYP2B seemed to be equally involved in metabolism. These results are in close agreement with those of Chaney and Carlson [19], who showed that 17.5 lM benzene metabolism in both rat liver and lung was inhibited by DDTC (300 lM), indicating that CYP2E1 is major isozyme involved in benzene metabolism. In their study benzene metabolism in liver was inhibited by 96% and in lung by 54%, compared to 92% and 41%, respectively, in our current study.…”

“…CYP2E1 was the P450 isozyme most involved at both 24 lM and 1000 lM benzene in hepatic microsomes, consistent with the published results of other researchers [19,36,37]. CYP2F and CYP2E1 were both involved in 24 lM benzene metabolism by rat pulmonary microsomes.…”

Benzene metabolism in human lung cell lines BEAS‐2B and A549 and cells overexpressing CYP2F1

“…The components of the organic layer were analyzed using a Shimadzu HPLC system with a Beckman octadecyl silane (ODS) column ( (or per 10 6 cells)/45 min. This time period was in keeping with our previous studies on tissues [16][17][18][29][30][31][32]. Since the primary metabolite was phenol with only traces of hydroquinone and catechol, derived from further metabolism of this metabolite, being identified, the results are expressed as total hydroxylated metabolite.…”

The Role of Metabolism and Specific Metabolites in Benzene-Induced Toxicity: Evidence and Issues

“……”

Cytochromes P450 involved with benzene metabolism in hepatic and pulmonary microsomes

“…At 1000 lM benzene, CYP2E1, CYP2F, and CYP2B seemed to be equally involved in metabolism. These results are in close agreement with those of Chaney and Carlson [19], who showed that 17.5 lM benzene metabolism in both rat liver and lung was inhibited by DDTC (300 lM), indicating that CYP2E1 is major isozyme involved in benzene metabolism. In their study benzene metabolism in liver was inhibited by 96% and in lung by 54%, compared to 92% and 41%, respectively, in our current study.…”

“…CYP2E1 was the P450 isozyme most involved at both 24 lM and 1000 lM benzene in hepatic microsomes, consistent with the published results of other researchers [19,36,37]. CYP2F and CYP2E1 were both involved in 24 lM benzene metabolism by rat pulmonary microsomes.…”

Benzene metabolism in human lung cell lines BEAS‐2B and A549 and cells overexpressing CYP2F1

“…The components of the organic layer were analyzed using a Shimadzu HPLC system with a Beckman octadecyl silane (ODS) column ( (or per 10 6 cells)/45 min. This time period was in keeping with our previous studies on tissues [16][17][18][29][30][31][32]. Since the primary metabolite was phenol with only traces of hydroquinone and catechol, derived from further metabolism of this metabolite, being identified, the results are expressed as total hydroxylated metabolite.…”

The Role of Metabolism and Specific Metabolites in Benzene-Induced Toxicity: Evidence and Issues

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