Comparison of Proteinase Activities in Squamous Cell Carcinoma, Basal Cell Epithelioma, and Seborrheic Keratosis

Tsuboi, Ryoji; Yamaguchi, Tomohiko; Kurita, Yoriyuki; Nakao, Hiroshi; Ogawa, Hideoki; Ishihara, Kazuyuki

doi:10.1111/1523-1747.ep12462121

Cited by 23 publications

(9 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the present study, cathepsin D epithelial and stromal scores were statistically significantly elevated in all the groups studied compared with the normal control group with the highest values in SCC. This result was in agreement with that of Tsuboi et al [18], who found high cathepsin D activity in the same investigated tumors, but with no significant difference, and suggested that no difference was found in the lysosomal functions of the tumors. The present study was also in agreement with a study carried out by Fröhlich et al [19], who found that in BCCs, cathepsins B, L, and D were increased.…”

Section: Discussionsupporting

confidence: 93%

Immunohistochemical expression of cathepsin D and matrix metalloproteinase-9 in common epidermal tumors

Hassan¹,

El-Ashmawy²,

Shareef³

et al. 2014

Journal of the Egyptian Womenʼs Dermatologic Society

View full text Add to dashboard Cite

BackgroundCathepsin D and matrix metalloproteinase-9 (MMP-9) are proteolytic enzymes that degrade connective tissue in the extracellular matrix and play a major role in the destruction of the basement membrane. Objective To explore immunohistochemical expression of cathepsin D and MMP-9 in basal cell carcinoma (BCC), squamous cell carcinoma (SCC) and seborrheic keratosis (SK). Patients and methodsThis retrospective study included 45 patients classified into the following groups: group I, 15 patients with SK; group II, 15 patients with BCC; group III, 15 patients with SCC; and group IV, control group, which included 10 age-matched and sex-matched individuals with normal healthy skin. All participants were subjected to hematoxylin and eosin stain and immunohistochemical staining for cathepsin D and MMP-9. ResultsA statistically significant increase in the intensity of expression of cathepsin D and MPP-9 in the SCC, BCC, and SKs was found in the patient groups than the control group. The expression of both markers was significantly higher in SCC than in BCC and SK. The expression of both markers was significantly higher in BCC with a high risk of recurrence than in those with a low risk of recurrence, and was significantly positively correlated with advancement in histological grade in SCC. ConclusionThe cytoplasmic expressions of cathepsin D and MMP-9 were prominent in stromal and epithelial cells of SCC than in BCC and SK. This pointed to the capability of SCC of invasion as well as metastatic potentiality than in BCC and SK.

show abstract

Section: Discussionsupporting

confidence: 93%

Immunohistochemical expression of cathepsin D and matrix metalloproteinase-9 in common epidermal tumors

Hassan¹,

El-Ashmawy²,

Shareef³

et al. 2014

Journal of the Egyptian Womenʼs Dermatologic Society

View full text Add to dashboard Cite

show abstract

“…In early studies in fibrin lysis assay, no statistical difference in the activity of PA from BCC and normal skin samples could be detected [49]. Moreover, in later studies, no significant difference in the biochemical activity or expression level of PA from skin samples of seborrheic keratosis, BCC, and squamous cell carcinoma was observed [48,68]. Comparative analysis of uPA mRNA content from different types of BCC revealed its presence only in the nodular subtype of BCC (in tumor islands, fibroblast-like stromal cells, and in the basal epidermal cell layer) [64].…”

Section: Discussionmentioning

confidence: 97%

Increased expression of uPA, uPAR, and PAI-1 in psoriatic skin and in basal cell carcinomas

et al. 2017

View full text Add to dashboard Cite

There is substantial evidence implicating the urokinase system in tissue remodeling during neo-vascularization, inflammation, tumor invasion, and metastasis. Regulated degradation of the extracellular matrix at the leading edge of migrating cells, mediated by uPA and uPAR, is required for tissue remodeling, invasiveness, and angiogenesis. Psoriasis and basal cell carcinoma (BCC) are the most common skin diseases. Pathogenesis of both of them is associated with keratinocyte hyperproliferation, inflammatory cell migration, and angiogenesis-processes in which the plasminogen system (uPA, uPAR, tPA, and PAI-1) plays a crucial role. In the present study, the comparative analysis of uPA, uPAR, tPA, and PAI-1 expression in the normal skin, in the biopsies of patients with psoriasis vulgaris, and BCC was carried out. uPA, uPAR, and PAI-1 expression was up-regulated in the epidermis of psoriatic skin and in tumor cells in BCC. Increased uPAR expression was detected in the derma of psoriatic lesions and in the stroma surrounding tumor cells in BCC. Increased expression of uPA in epidermal cells in psoriasis and in tumor cells in BCC suggests an important role of the uPA system for aggressively proliferating and invading cells of epidermal origin. A possible activation of the stroma, as a result of uPA-uPAR interaction between tumor cells and the surrounding stroma, is suggested.

show abstract

“…On the contrary, there was a report in which were reported that basocellular epitheliomas produce a lot of collagenase but do not invade [21]. However, whether collagenase is related to the invasiveness of the tumour or not is still controversial.…”

Section: Discussionmentioning

confidence: 99%

Type IV collagenase activity and cavernous sinus invasion in human pituitary adenomas

et al. 1996

View full text Add to dashboard Cite

Pituitary adenomas are regarded generally as benign tumours, but some of them can invade the cavernous sinus. On the other hand, matrix metalloproteinase-9 (MMP-9), which is a type IV collagenase, recently has been found to be expressed in matastases and to be related to the invasiveness of various malignant tumours including brain tumours. In order to investigate some characteristic features of pituitary adenomas which invade the cavernous sinus, we examined immunohistological studies for MMP-9 in seven pituitary adenomas for type IV collagen in a dura mater and assayed for type IV collagenase activity in seven adenomas using type IV collagen labelled with fluorescein isothiocyaniate (FITC). We found immunopositive adenoma cells for MMP-9 in all invasive adenoma and immunopositive spindle like cells for type IV collagen in the dura mater. All three invasive adenomas had high levels of type IV collagenase activity (0.57-0.72 U/ml), but the four adenomas which did not invade the cavernous sinus had low levels of type IV collagenase activity (0.0-0.10 U/ml). These results suggest that the level of type IV collagenase activity in a pituitary adenoma may be related to its ability to invade the cavernous sinus.

show abstract

Comparison of Proteinase Activities in Squamous Cell Carcinoma, Basal Cell Epithelioma, and Seborrheic Keratosis

Cited by 23 publications

References 16 publications

Immunohistochemical expression of cathepsin D and matrix metalloproteinase-9 in common epidermal tumors

Immunohistochemical expression of cathepsin D and matrix metalloproteinase-9 in common epidermal tumors

Increased expression of uPA, uPAR, and PAI-1 in psoriatic skin and in basal cell carcinomas

Type IV collagenase activity and cavernous sinus invasion in human pituitary adenomas

Contact Info

Product

Resources

About