Comparison of protein patterns of Listeria monocytogenes grown in biofilm or in planktonic mode by proteomic analysis

Trémoulet, F

doi:10.1016/s0378-1097(02)00571-2

Cited by 106 publications

(51 citation statements)

References 12 publications

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“…Previous studies have focused on comparing physiological features between biofilm and planktonic populations for different bacteria [9,14,33,34]; however, none of these studies have addressed this question by combining proteomic and FT-IR spectroscopy techniques.…”

Section: Discussionmentioning

confidence: 99%

Proteome approaches combined with Fourier transform infrared spectroscopy revealed a distinctive biofilm physiology in Bordetella pertussis

et al. 2008

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Proteome analysis was combined with whole-cell metabolic fingerprinting to gain insight into the physiology of mature biofilm in Bordetella pertussis, the agent responsible for whooping cough. Recent reports indicate that B. pertussis adopts a sessile biofilm as a strategy to persistently colonize the human host. However, since research in the past mainly focused on the planktonic lifestyle of B. pertussis, knowledge on biofilm formation of this important human pathogen is still limited. Comparative studies were carried out by combining 2-DE and Fourier transform infrared (FT-IR) spectroscopy with multivariate statistical methods. These complementary approaches demonstrated that biofilm development has a distinctive impact on B. pertussis physiology. Results from MALDI-TOF/MS identification of proteins together with results from FT-IR spectroscopy revealed the biosynthesis of a putative acidic-type polysaccharide polymer as the most distinctive trait of B. pertussis life in a biofilm. Additionally, expression of proteins known to be involved in cellular regulatory circuits, cell attachment and virulence was altered in sessile cells, which strongly suggests a significant impact of biofilm development on B. pertussis pathogenesis. In summary, our work showed that the combination of proteomics and FT-IR spectroscopy with multivariate statistical analysis provides a powerful tool to gain further insight into bacterial lifestyles.

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Section: Discussionmentioning

confidence: 99%

Proteome approaches combined with Fourier transform infrared spectroscopy revealed a distinctive biofilm physiology in Bordetella pertussis

et al. 2008

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“…This map contains proteins belonging to each of the four major functional classes defined for L. monocytogenes EGDe (14). Only 6 of the 33 proteins identified, Fri (9,17,19,31,32), GAPDH (8), Pfk (17,39), Pgm (8), TufA (8), and the mannose-specific phosphotransferase system enzyme IIAB (8,17,34), have been identified previously in L. monocytogenes by 2-D analysis.…”

Section: Discussionmentioning

confidence: 99%

Development of a Listeria monocytogenes EGDe Partial Proteome Reference Map and Comparison with the Protein Profiles of Food Isolates

Ramnath

Rechinger²,

Jänsch

et al. 2003

Appl Environ Microbiol

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A partially annotated proteome reference map of the food pathogen Listeria monocytogenes was developed for exponentially growing cells under standardized, optimal conditions by using the sequenced strain EGDe (serotype 1/2a) as a model organism. The map was developed by using a reproducible total protein extraction and two-dimensional (2-D) polyacrylamide gel electrophoresis analysis procedure, and it contained 33 identified proteins representing the four main protein functional classes. In order to facilitate analysis of membrane proteins, a protein compartmentalization procedure was assessed. The method used provided partial fractionation of membrane and cytosolic proteins. The total protein 2-D profiles of three serotype 1/2a strains and one serotype 1/2b strain isolated from food were compared to the L. monocytogenes EGDe proteome. An average of 13% of the major protein spots in the food strain proteomes were not matched in the strain EGDe proteome. The variation was greater for the less intense spots, and on average 28% of these spots were not matched. Two of the proteins identified in L. monocytogenes EGDe were missing in one or more of the food isolates. These two proteins were proteins involved in the main glycolytic pathway and in metabolism of coenzymes and prosthetic groups. The two corresponding genes were found by PCR amplification to be present in the four food isolates. Our results show that the L. monocytogenes EGDe reference map is a valuable starting point for analyses of strains having various origins and could be useful for analyzing the proteomes of different isolates of this pathogen.

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“…Consistent with the differentiation of cells in biofilms, sessile cells and planktonic cells show different gene expression profiles (6,21,29,30,32,34,(38)(39)(40). Furthermore, gene expression changes during biofilm development (21, 29).…”

mentioning

confidence: 88%

Identification of a Novel Two-Component System inStreptococcus gordoniiV288 Involved in Biofilm Formation

Zhang¹,

Lei²,

Khammanivong³

et al. 2004

Infect Immun

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Streptococcus gordonii is a pioneer colonizer of the teeth, contributing to the initiation of the oral biofilm called dental plaque. To identify genes that may be important in biofilm formation, a plasmid integration library of S. gordonii V288 was used. After screening for in vitro biofilm formation on polystyrene, a putative biofilm-defective mutant was isolated. In this mutant, pAK36 was inserted into a locus encoding a novel two-component system (bfr [biofilm formation related]) with two cotranscribed genes that form an operon. bfrA encodes a putative response regulator, while bfrB encodes a receptor histidine kinase. The bfr mutant and wild-type strain V288 showed similar growth rates in Todd-Hewitt broth (THB). A bfr-cat fusion strain was constructed. During growth in THB, the reporter activity (chloramphenicol acetyltransferase) was first detected in mid-log phase and reached a maximum in stationary phase, suggesting that transcription of bfr was growth stage dependent. After being harvested from THB, the bfr mutant adhered less effectively than did wild-type strain V288 to saliva-coated hydroxyapatite (sHA). To simulate pioneer colonization of teeth, S. gordonii V288 was incubated with sHA for 4 h in THB with 10% saliva to develop biofilms. RNA was isolated, and expression of bfrAB was estimated. In comparison to that of cells grown in suspension (free-growing cells), bfr mRNA expression by sessile cells on sHA was 1.8-fold greater and that by surrounding planktonic cells was 3.5-fold greater. Therefore, bfrAB is a novel two-component system regulated in association with S. gordonii biofilm formation in vitro.Dental plaque is a natural polymicrobial biofilm formed on saliva-coated tooth surfaces. One of several pioneer colonizers that initiate the formation of dental plaque, Streptococcus gordonii is an oral commensal that causes no known disease in the oral cavity (20). If it enters the blood, however, S. gordonii and other viridans group streptococci behave as pathogens to cause infective endocarditis (1, 18). S. gordonii must adhere to and colonize tissue surfaces in vivo in flowing blood or saliva. Alternatively, the microorganism will be cleared by the reticuloendothelial system or by swallowing, respectively. Many laboratories seek to understand how this organism adheres and initiates biofilm formation.Biofilms grow and form microscopic communities of distinct and reproducible architecture (14) by a multistep process (4,14,33). Consistent with the differentiation of cells in biofilms, sessile cells and planktonic cells show different gene expression profiles (6,21,29,30,32,34,(38)(39)(40). Furthermore, gene expression changes during biofilm development (21, 29). To understand the molecular mechanisms and the evolutionary basis of survival of these species, key gene regulation pathways of biofilm formation must be elucidated.S. gordonii adheres to specific surfaces in the host to colonize and form biofilms (8). Properties of surfaces on which biofilms develop can also affect the structure of S. gord...

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Comparison of protein patterns of Listeria monocytogenes grown in biofilm or in planktonic mode by proteomic analysis

Cited by 106 publications

References 12 publications

Proteome approaches combined with Fourier transform infrared spectroscopy revealed a distinctive biofilm physiology in Bordetella pertussis

Proteome approaches combined with Fourier transform infrared spectroscopy revealed a distinctive biofilm physiology in Bordetella pertussis

Development of a Listeria monocytogenes EGDe Partial Proteome Reference Map and Comparison with the Protein Profiles of Food Isolates

Identification of a Novel Two-Component System inStreptococcus gordoniiV288 Involved in Biofilm Formation

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