Comparison of protein adsorption patterns onto differently charged hydrophilic superparamagnetic iron oxide particles obtainedin vitro andex vivo

Abstract

Protein adsorption patterns of superparamagnetic iron oxides (SPIO) were evaluated by two-dimensional electrophoresis (2-DE) after in vitro incubation of the particles in plasma or serum. SPIO particles having positive (MKK 1211), negative (MKA 1211), or neutral (MKG 1411) charge were used. Protein adsorption patterns of different charged SPIO particles acquired in vitro and recollected 5 min after intravenous injection into rats (ex vivo) were compared. For the uncharged MKG 1411 particles, the differences of protein adsorption patterns were negligible and only minor differences were found for the negatively charged MKA 1211 and positively charged MKK 1211 particles. A good correlation between in vitro and ex vivo data could be shown. For the evaluation of protein adsorption patterns of SPIO particles determining organ distribution and allowing estimation of site-specific delivery (drug targeting), the currently used protocol for 2-DE analysis could be confirmed.

Effectiveness of micron‐sized superparamagnetic iron oxide particles as markers for detection of migration of bone marrow‐derived mesenchymal stromal cells in a stroke model

“…Similarly, free MPIOs injected in the tail vein could also be engulfed by macrophages and transported to the brain, although the possibility exists that their uptake by macrophages might be prevented by the zeta potential of the macrophages as well as the MPIOs. The zeta potential is an indicator of the degree of repulsion between adjacent cells, and it is significant since it has been demonstrated that since both MPIOs (35) and macrophages (36) have a high degree of negative charge, the uptake of MPIOs by macrophages is inhibited in certain situations. The possibility also exists that an initial accumulation of MPIOs at the lesion site might be due to the enhanced permeability and retention (EPR) effect, whereby certain sizes of molecules tend to accumulate in certain tissue types (37, 38), and these MPIOs may have subsequently been taken up by the local activated macrophages.…”

The Role of the Protein Corona in Fiber Structure-Activity Relationships

“…To answer the question, if there are certain fiber-specific characteristics within the protein-corona of fibers, we compared the "top-ten" or "hit list" of the proteins found on fibers and other nanomaterials of different shapes and materials: i.e., CNTs, silica nanoparticles [87,88], gold nanoparticles [89][90][91], polymeric particles [27,88,92], and iron oxide nanoparticles [93,94]. Despite the differences in morphology (shape and size), surface characteristics and material composition, several proteins (e.g., albumin, apolipoprotein AI and E, fibrinogen, fibronectin, vitronectin, complement factor C3, α2-macroglobulin) were found on nearly all protein coronas investigated.…”

Lymph node staging using dedicated magnetic resonance contrast agents—the accumulation mechanism revisited

“…Muhler reports that LN uptake of carboxydextran-coated, 20 nm (mean) sized USPIOs (Table 1, DDM128N fraction) in LN was similar before and after complement depletion with cobra venom factor and is, therefore, independent of C3 binding. 16 In another report, more than 10 plasma proteins were found to bind to the surface of negative, neutral, or positive SPIOs by 2D-gel electrophoresis, and C3 was not one of these proteins 17 (Table 1, SPIO-Lind). The protein levels differed significantly between the particle variants.…”

“…In another report, more than 10 plasma proteins were found to bind to the surface of negative, neutral, or positive SPIOs by 2D‐gel electrophoresis, and C3 was not one of these proteins (Table , SPIO‐Lind). The protein levels differed significantly between the particle variants.…”