Comparison of polysaccharide and poly(ethylene glycol) coatings for reduction of protein adsorption on polystyrene surfaces

Österberg, E.; Bergström, Karin; Holmberg, Krister; Riggs, Jennifer A.; Alstine, James M. Van; Schuman, Thomas P.; Burns, Norman; Harris, J. Milton

doi:10.1016/0927-7757(93)80112-r

Cited by 135 publications

(121 citation statements)

References 14 publications

Supporting

Mentioning

119

Contrasting

Order By: Relevance

“…Thus, in analogy to the mannitol SAMs prepared by Mrksich and co-workers [23] and to the dextran surfaces, [24,25] these PG derivatives are yet another exception to the correlation between protein resistance and the absence of hydrogen-bond donors. [16,22] Our incentive for the synthesis of the compound MeO-PG±Thioc 5 d in which the majority of the acidic hydrogen atoms were substituted by a CH 3 group was to verify whether these criteria also apply to PG derivatives.…”

Section: Resultsmentioning

confidence: 98%

Self‐Assembled Monolayers of Dendritic Polyglycerol Derivatives on Gold That Resist the Adsorption of Proteins

Siegers

Biesalski

Haag

2004

Chemistry A European J

280

275

View full text Add to dashboard Cite

Highly protein-resistant, self-assembled monolayers (SAMs) of dendritic polyglycerols (PGs) on gold can easily be obtained by simple chemical modification of these readily available polymers with a surface-active disulfide linker group. Several disulfide-functionalized PGs were synthesized by N,N'-dicyclohexylcarbodiimide-mediated ester coupling of thioctic acid. Monolayers of the disulfide-functionalized PG derivatives spontaneously form on a semitransparent gold surface and effectively prevent the adsorption of proteins, as demonstrated by surface plasmon resonance (SPR) kinetic measurements. A structure-activity relationship relating the polymer architecture to its ability to effectuate protein resistance has been derived from results of different surface characterization techniques (SPR, attenuated total reflectance infrared (ATR-IR), and contact-angle measurements). Dendritic PGs combine the characteristic structural features of several highly protein-resistant surfaces: a highly flexible aliphatic polyether, hydrophilic surface groups, and a highly branched architecture. PG monolayers are as protein resistant as poly(ethylene glycol) (PEG) SAMs and are significantly better than dextran-coated surfaces, which are currently used as the background for SPR spectroscopy. Due to the higher thermal and oxidative stability of the bulk PG as compared to the PEG and the easy accessibility of these materials, dendritic polyglycerols are novel and promising candidates as surface coatings for biomedical applications.

show abstract

Section: Resultsmentioning

confidence: 98%

Self‐Assembled Monolayers of Dendritic Polyglycerol Derivatives on Gold That Resist the Adsorption of Proteins

Siegers

Biesalski

Haag

2004

Chemistry A European J

280

275

View full text Add to dashboard Cite

show abstract

“…Common features of nonfouling ͑i.e., protein-resistant͒ surfaces are their hydrophilicity, their charge neutrality, as well as the presence of hydrogen bond acceptors, but absence of hydrogen bond donor groups. 5 Several ways to create protein-resistant surfaces have been proposed: poly͑ethylene glycol͒ chemistry, 2,5,6 functionalized alkanethiols, [7][8][9] supported phospholipid bilayers, 10,11 polysaccharide chemistry, [12][13][14][15] and others.…”

Section: Introductionmentioning

confidence: 99%

Poly(l-lysine)-grafted-poly(ethylene glycol)-based surface-chemical gradients. Preparation, characterization, and first applications

et al. 2006

View full text Add to dashboard Cite

A simple dipping process has been used to prepare PEGylated surface gradients from the polycationic polymer poly(l-lysine), grafted with poly(ethylene glycol) (PLL-g-PEG), on metal oxide substrates, such as TiO2 and Nb2O5. PLL-g-PEG coverage gradients were prepared during an initial, controlled immersion and characterized with variable angle spectroscopic ellipsometry and x-ray photoelectron spectroscopy. Gradients with a linear change in thickness and coverage were generated by the use of an immersion program based on an exponential function. These single-component gradients were used to study the adsorption of proteins of different sizes and shapes, namely, albumin, immunoglobulin G, and fibrinogen. The authors have shown that the density and size of defects in the PLL-g-PEG adlayer determine the amount of protein that is adsorbed at a certain adlayer thickness. In a second step, single-component gradients of functionalized PLL-g-PEG were backfilled with nonfunctionalized PLL-g-PEG to generate two-component gradients containing functional groups, such as biotin, in a protein-resistant background. Such gradients were combined with a patterning technique to generate individually addressable spots on a gradient surface. The surfaces generated in this way show promise as a useful and versatile biochemical screening tool and could readily be incorporated into a method for studying the behavior of cells on functionalized surfaces.

show abstract

“…Previous studies demonstrated that surface-immobilized dextran reduces cell adhesion and spreading in vitro. [12][13][14][15][16] Further work developed methods for immobilizing bioactive peptides to low cell-adhesive dextran monolayer coatings. 17 The substrates were shown to promote biospecific cell adhesion for vascular implant applications.…”

Section: Introductionmentioning

confidence: 99%

In vitro assessment of bioactive coatings for neural implant applications

Massia

Holecko

Ehteshami

2003

J Biomedical Materials Res

View full text Add to dashboard Cite

Recent efforts in our laboratory have focused on developing methods for immobilizing bioactive peptides to low cell-adhesive dextran monolayer coatings and promoting biospecific cell adhesion for biomaterial implant applications. In the current study, this dextran-based bioactive coating technology was developed for silicon, polyimide, and gold, the base materials utilized to fabricate our prototype neural implants. Chemical composition of all modified surfaces was verified by X-ray photoelectron spectroscopy (XPS). We observed that surface-immobilized dextran supported very little cell adhesion in vitro (24-h incubation with serum-supplemented medium) on all base materials. Inactive nonadhesion-promoting Gly-Arg-Ala-Asp-Ser-Pro peptides immobilized on dextran-coated materials promoted adhesion and spreading at low levels not significantly different from dextran-coated substrates. Arg-Gly-Asp (RGD) peptide-grafted surfaces were observed to promote substantial fibroblast and glial cell adhesion with minimal PC12 (neuronal cell) adhesion. In contrast, dextran-coated materials with surface-grafted laminin-based, neurite-promoting Ile-Lys-Val-Ala-Val (IKVAV) peptide promoted substantial neuron cell adhesion and minimal fibroblast and glial cell adhesion. It was concluded that neuron-selective substrates are feasible using dextran-based surface chemistry strategies. The chemical surface modifications could be utilized to establish a stable neural tissue-implant interface for longterm performance of neural prosthetic devices.

show abstract

Comparison of polysaccharide and poly(ethylene glycol) coatings for reduction of protein adsorption on polystyrene surfaces

Cited by 135 publications

References 14 publications

Self‐Assembled Monolayers of Dendritic Polyglycerol Derivatives on Gold That Resist the Adsorption of Proteins

Self‐Assembled Monolayers of Dendritic Polyglycerol Derivatives on Gold That Resist the Adsorption of Proteins

Poly(l-lysine)-grafted-poly(ethylene glycol)-based surface-chemical gradients. Preparation, characterization, and first applications

In vitro assessment of bioactive coatings for neural implant applications

Contact Info

Product

Resources

About