Comparison of physico-chemical characteristics of four laccases from different basidiomycetes

Extracellular Laccase Produced by an Edible Basidiomycetous Mushroom,Grifola frondosa: Purification and Characterization

“…1B) and Gel-filtration (data not shown), indicating a monomeric structure. This finding is consistent with typical fungal laccases, which are monomeric proteins of approximately 60-70 kDa, 34) such as Pleurotus eryngii (61 and 65 kDa), 35) Trametes hirsuta (70 kDa), 36) and Polyporus pinsitus (66 kDa). 37) Analytical isoelectric focusing revealed that Lac 1 is an acidic protein having a pI lower than 3.5.…”

Stable ‘Floating' Air Diffusion Biocathode Based on Direct Electron Transfer Reactions Between Carbon Particles and High Redox Potential Laccase

“…Obviously, half-wave potentials registered in our studies ( Figure 3) coincide well with the redox potential of Trametes hirsuta Lc, measured to be 780 mV ± 10 mV versus NHE at pH about 6 [39,42]. We also noticed that the initial parts of both curves, 1 and 2, are almost identical, pointing to the fact that at low overpotentials, the bioelectrochemical reaction of O 2 reduction occurs in the kinetic mode, i.e.…”

“…The Lc was isolated and purified from a culture medium according to Ref. [39]. The homogeneous preparation of the enzyme, as judged from SDS-PAGE, with a protein concentration of about 15 mg mL -1 was stored in 100 mM phosphate buffer, pH 6.5 at -20°C.…”

Wood production, wood technology, and biotechnological impacts

“…Nevertheless, currently applied incubation times of 30 and more minutes are too long to be economical. An important hindrance in reaction efficiency is its low redox-potential of the enzymes (Solomon et al 1992, Shleev et al 2004. Screening for mutations by modern molecular biological techniques can yield enzymes with higher redox-potential for recombinant production , Zumarraga et al 2007; further reading in Chapter 19 of this book).…”