Comparison of particle clearance and macrophage phagosomal motion in liver and lungs of rats

Weinstock, Shelley B.; Brain, Joseph D.

doi:10.1152/jappl.1988.65.4.1811

Cited by 20 publications

(11 citation statements)

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“…16 This noninvasive technique can be used to repeatedly study the intracellular (phagosomal) movement within macrophages. It is an established method to assess cytoskeletal function within lung [17][18][19][20][21] and liver macrophages in intact animals. 19,22,23 When injected intravenously (IV), ferromagnetic iron oxide particles are rapidly cleared from the circulation, and the majority of them are retained in macrophages that have access to the circulation, such as in the liver and spleen.…”

Section: Introductionmentioning

confidence: 99%

Effects of zinc oxide nanoparticles on Kupffer cell phagosomal motility, bacterial clearance, and liver function

Watson¹,

Molina²,

Louzada³

et al. 2015

IJN

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Background Zinc oxide engineered nanoparticles (ZnO ENPs) have potential as nanomedicines due to their inherent properties. Studies have described their pulmonary impact, but less is known about the consequences of ZnO ENP interactions with the liver. This study was designed to describe the effects of ZnO ENPs on the liver and Kupffer cells after intravenous (IV) administration. Materials and methods First, pharmacokinetic studies were conducted to determine the tissue distribution of neutron-activated 65 ZnO ENPs post-IV injection in Wistar Han rats. Then, a noninvasive in vivo method to assess Kupffer cell phagosomal motility was employed using ferromagnetic iron particles and magnetometry. We also examined whether prior IV injection of ZnO ENPs altered Kupffer cell bactericidal activity on circulating Pseudomonas aeruginosa . Serum and liver tissues were collected to assess liver-injury biomarkers and histological changes, respectively. Results We found that the liver was the major site of initial uptake of 65 ZnO ENPs. There was a time-dependent decrease in tissue levels of 65 Zn in all organs examined, refecting particle dissolution. In vivo magnetometry showed a time-dependent and transient reduction in Kupffer cell phagosomal motility. Animals challenged with P . aeruginosa 24 hours post-ZnO ENP injection showed an initial (30 minutes) delay in vascular bacterial clearance. However, by 4 hours, IV-injected bacteria were cleared from the blood, liver, spleen, lungs, and kidneys. Seven days post-ZnO ENP injection, creatine phosphokinase and aspartate aminotransferase levels in serum were significantly increased. Histological evidence of hepatocyte damage and marginated neutrophils were observed in the liver. Conclusion Administration of ZnO ENPs transiently inhibited Kupffer cell phagosomal motility and later induced hepatocyte injury, but did not alter bacterial clearance from the blood or killing in the liver, spleen, lungs, or kidneys. Our data show that diminished Kupffer cell organelle motion correlated with ZnO ENP-induced liver injury.

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Section: Introductionmentioning

confidence: 99%

Effects of zinc oxide nanoparticles on Kupffer cell phagosomal motility, bacterial clearance, and liver function

Watson¹,

Molina²,

Louzada³

et al. 2015

IJN

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“…They are then ingested by lung macrophages (36). In species lacking PIMs, such asthe rat, these particles are ingested by hepatic and splenic macrophages after intravenous injection (37). However, when species with abundant PIMs are studied, these lung cells can be readily labeled by intravenous injection ofmagnetic dust.…”

Section: Magnetometric Methods For Measuring Macrophage Motilitymentioning

confidence: 99%

Mechanisms, measurement, and significance of lung macrophage function.

Brain

1992

Environ Health Perspect

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Macrophages exist throughout the body. They have critical roles in the peritoneal cavity, bone marrow, skin, spleen, liver, and elsewhere. Their migratory patterns, phagocytic behavior, immunoloc roles, and secretory potential are pivotal to both defense mechanisms and to the pathogenesis ofdisease. Macrophages of macrophages and resulting motile events are key in understanding the role of macrophages in relation to particle phagocytosis, translocation, and solubilization.

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“…Particle clearance has been compared using magnetometry in two different 20 organs, the lungs and liver (45) (45).…”

Section: Lawmentioning

confidence: 99%

“…No net cell motion for phagocytosis is needed since the particles are presented to these cells by the circulating blood. The rates of particle clearance from each organ have been compared (45). Pulmonary clearance includes not only in situ solubilization of iron oxide particles, but may also represent bulk, mechanical transport from the respiratory tract by mucociliary transport or macrophage migration.…”

Section: Lawmentioning

confidence: 99%

See 1 more Smart Citation

In Vivo Evaluation of Chemical Biopersistence of Nonfibrous Inorganic Particles

Brain¹,

Godleski²,

Kreyling³

1994

Environmental Health Perspectives

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The lung's response to deposited particles may depend upon the physical-chemical properties of the particles, the amount initially deposited, and the persistence of the particles. Clearance involves mucociliary transport as well as the action of phagocytic cells in nonciliated regions of the lung. Depending on the animal species studied, particle type, and particle load, inorganic materials are ingested by macrophages on alveolar surfaces with half-times of 0.6 to 7 hr. Particle-laden macrophages may migrate to airways, but we believe that an important mechanism of clearance is the dissolution of particles within alveolar macrophages and the subsequent translocation of dissolved materials to the blood. Particle dissolution in situ has long been recognized but was often thought to be carried out extracellularly in the alveolar lining layer, airway mucus, or interstitial fluid. However, many particles such as cobalt oxide or iron oxide which dissolve very little in simulated lung fluid, are solubilized more rapidly within alveolar macrophages. Clearance of particles from the lungs can be followed by a number of techniques, both invasive and noninvasive. The approaches vary in expense and resolution, and can be directed toward quantifying mechanical removal of particles versus their intracellular dissolution. Noninvasive methods permit repeated measurements of particle retention in the lungs of the same animal or human and thus allow replications and serial measurements. Greater precision with respect to the sites of retention and redistribution is achieved with quantitative morphometric methods that utilize fixation followed by physically dividing the respiratory tract into individual pieces. Microwave drying or slam-freezing can eliminate the possibility of significant particle redistribution or loss of particles and dissolved elements during tissue processing. Detection of particles and therefore evidence of clearance can rely upon any distinctive property of the aerosol. Particles may be radioactive, fluorescent, or magnetic, or may have a characteristic visual appearance. Detection techniques include radiography, analyses of radioactivity, magnetometry, and microscopic approaches such as fluorescence and confocal microscopy, X-ray emission analysis, and electron energy loss spectrometry (EELS). Using these approaches, considerable evidence has been accumulated to conclude that particle dissolution in situ within alveolar macrophages and subsequent absorption by the circulation, rather than bulk transport, is the dominant mechanism for the long-term clearance of many insoluble minerals from the lungs. -Environ Health Perspect 102(Suppl 5): 119-125 (1994)

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Comparison of particle clearance and macrophage phagosomal motion in liver and lungs of rats

Cited by 20 publications

References 0 publications

Effects of zinc oxide nanoparticles on Kupffer cell phagosomal motility, bacterial clearance, and liver function

Effects of zinc oxide nanoparticles on Kupffer cell phagosomal motility, bacterial clearance, and liver function

Mechanisms, measurement, and significance of lung macrophage function.

In Vivo Evaluation of Chemical Biopersistence of Nonfibrous Inorganic Particles

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