Comparison of nucleated red blood cell count with four commercial hematological analyzers

Pipitone, Silvia; Buonocore, Ruggero; Gennari, Daniela Treveri; Lippi, Giuseppe

doi:10.1515/cclm-2014-1211

Cited by 9 publications

(9 citation statements)

References 8 publications

(11 reference statements)

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“…In addition, while analysing NRBC positive patients without hematologic diseases, blood cytokine profile suggests that NRBC may be considered a parameter that sums inflammatory injuries. 26 Although peripheral platelet counts do not correlate with NRBC, we have suggested that the differences in platelet index may have a greater impact on PID suspicion in this study.…”

Section: Discussioncontrasting

confidence: 49%

Is there clinical value in counting nucleated red blood cells and platelet indices in primary immunodeficiency disease?

Ekici-Günay¹,

Çakir²,

Çelik³

2017

Turk J Pediatr

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Ekici-Günay N, Çakır I, Çelik T. Is there clinical value in counting nucleated red blood cells and platelet indices in primary immunodeficiency disease? Turk J Pediatr 2017; 59: 657-663. Infections are the most common presentation of primary immunodeficiency diseases (PIDs). The increase of nucleated red blood cell (NRBC) count is interpreted as a systemic inflammatory response. Platelets play an important role in the pathogenesis of inflammatory disease. The relationship of platelet indices (PIs) and disease activity have been demonstrated in various inflammatory diseases. The aims of this study was to evaluate and compare NRBC and platelet/lymphocyte ratio (PLR), PIs [mean platelet volume (MPV), platelet distribution width (PDW), platelet large cell ratio (PLCR)] with a possible indirect inflammatory marker in children with PIDs. Data were recorded retrospectively from 66 PIDs patients, < 16 years of age. The relationships between peripheral NRBC, C-reactive protein (CRP) and PIs were analyzed. NRBC was positively correlated with CRP (p < 0.037), white blood cells (WBC) (p < 0.020), PLR (p < 0.044), PDW (p < 0.037) and PLCR (p < 0.001) and it was negatively correlated with platelet distribution width (PDW) (p < 0.036) in PIDs patients. A cutoff level of 0.80% NRBC, ≥15.55% PDW, ≥8.65 MPV and ≥43.67 PLR showed the best performance to predict PIDs, with 81% sensitivity, 27% specificity; 61% sensitivity, 37% specificity; 70% sensitivity, 43% specificity; 54% sensitivity, 40% specificity, respectively. Our results suggested that these indices may be used as auxillary diagnostic markers of PIDs with positive NRBC, showing more meaningful results than those known as the traditional infection markers for PIDs prediction. Elevated NRBC and MPV and low PDW are associated with infections and could be helpfull in the early diagnostic susception of PIDs. They can be used as rapidly accessible parameters for awareness of PIDs. These markers are easy to use in daily practice and without extra costs.

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Section: Discussioncontrasting

confidence: 49%

Is there clinical value in counting nucleated red blood cells and platelet indices in primary immunodeficiency disease?

Ekici-Günay¹,

Çakir²,

Çelik³

2017

Turk J Pediatr

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“…reported that in Sysmex XN‐module, NRBC showed a high correlation with manual differential, Lippi et al . found an overall agreement between OM and BC‐6800, and Pipitone et al . found statistically significant differences in the total number of NRBC between ADVIA 2120i and OM, whereas the NRBC count did not differ in the comparisons of BC‐6800 and OM.…”

Section: Discussionmentioning

confidence: 97%

“…Several published studies indicated excellent performances in terms of precision and accuracy of automated NRBC count and a good degree of harmonization for the absolute NRBC count among the different HAs …”

Section: Introductionmentioning

confidence: 99%

Performance evaluation of the automated nucleated red blood cell count of five commercial hematological analyzers

Rin

Vidali²,

Balboni³

et al. 2017

Int J Lab Hematology

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“…The second method, the flow cytometry (FC) method, has been proposed as a precise reference method for the NRBC count; however, this method requires expensive instrumentation, sophisticated operational skills, laborious sample preparation, and time‐consuming analysis . Recently, a new generation of automated HAs was developed to enumerate the NRBC concentration in blood with excellent precision and accuracy, depending on varying technologies and methods …”

Section: Introductionmentioning

confidence: 99%

“…11 Recently, a new generation of automated HAs was developed to enumerate the NRBC concentration in blood with excellent precision and accuracy, depending on varying technologies and methods. 12,13 At the Central Laboratory of King Chulalongkorn Memorial Hospital (KCMH), we provide routine complete blood count (CBC) services totaling over 350 000 tests per year, and approximately 50% of these tests are sent for review by MM because of abnormal flagging by automated HAs. The NRBC is one of the most frequent abnormal morphology flags.…”

mentioning

confidence: 99%

Automated nucleated red blood cell count using the Mindray BC‐6800 hematology analyzer

Houyhongthong

Nunphuak

Sripatumtong

et al. 2018

Int J Lab Hematology

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The Mindray CAL8000 automated hematology system, which is composed of the BC-6800 and the SC-120, yields a precise NRBC count and can replace the traditional MM method for obtaining accurate and reproducible NRBC counts in high-value samples, such as patient monitoring samples used to determine the necessity of transfusion therapy in thalassemia patients. Moreover, this method offers several advantages, including a faster turnaround time, labor savings, and cost effectiveness.

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Comparison of nucleated red blood cell count with four commercial hematological analyzers

Cited by 9 publications

References 8 publications

Is there clinical value in counting nucleated red blood cells and platelet indices in primary immunodeficiency disease?

Is there clinical value in counting nucleated red blood cells and platelet indices in primary immunodeficiency disease?

Performance evaluation of the automated nucleated red blood cell count of five commercial hematological analyzers

Automated nucleated red blood cell count using the Mindray BC‐6800 hematology analyzer

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