Comparison of nitrogen content amino acid composition and glucosamine content of cell walls of various chlorococcalean algae

Burczyk, J.; Smietana, B.; Termińska-Pabis, K.; Zych, Maria; Kowalowski, P

doi:10.1016/s0031-9422(99)00063-1

Cited by 50 publications

(32 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The composition of the cell wall among different Chlorella species is extremely diverse but more uniform through related species (Takeda 1993). Also, some Chlorella and Scenedesmus species may contain resistant biopolymer algaenan as part of their wall (Burczyk et al 1999). Additionally, these photosynthetic organisms have high metabolic flexibility and are capable of both mixotrophy and heterotrophy (C. sorokiniana (Bohutskyi et al 2014b, c;Kim et al 2013a, b;Ogbonna et al 2000;Rosenberg et al 2014;Wan et al 2012) and Scenedesmus Hodaifa et al 2009;Zhang et al 2013)).…”

Section: Discussionmentioning

confidence: 99%

Bioprospecting of microalgae for integrated biomass production and phytoremediation of unsterilized wastewater and anaerobic digestion centrate

Bohutskyi

Liu

Nasr

et al. 2015

Appl Microbiol Biotechnol

111

View full text Add to dashboard Cite

Eighteen microalgae, including two local isolates, were evaluated for their ability to grow and remove nutrients from unsterilized primary or secondary wastewater effluents as well as wastewater supplemented with nutrient-rich anaerobic digester centrate (ADC). Most of the tested species except several phylogenetically clustered Chlorella sorokiniana including local isolates and Scenedesmus strains were unable to grow efficiently. This may reflect the presence of certain genetic traits important for robust growth in the unsterilized wastewater. The maximum algal-specific growth rates and biomass density obtained in these bacterial-contaminated cultures were in the range of 0.8-1 day(-1) and 250-350 mg L(-1), respectively. ADC supplementation was especially helpful to biologically treated secondary effluent with its lower initial macronutrient and micronutrient content. As a result of algal growth, total nitrogen and orthophosphate levels were reduced by as much as 90 and 70 %, respectively. Biological assimilation was estimated to be the main mechanism of nitrogen removal in primary and secondary effluents with ammonia volatilization and bacterial nitrification-denitrification contributing for cultures supplemented with ADC. Assimilation by algae served as the principal mechanism of orthophosphate remediation in secondary wastewater cultures, while chemical precipitation appeared also to be important for orthophosphate removal in primary wastewater. Overall, cultivation of microalgae in primary and primary + 5 % ADC may be more favorable from an economical and sustainability perspective due to elimination of the costly and energy-intensive biological treatment step. These findings demonstrate that unsterilized wastewater and ADC can serve as critical nutrient sources for biomass generation and that robust microalgae can be potent players in wastewater phytoremediation.

show abstract

Section: Discussionmentioning

confidence: 99%

Bioprospecting of microalgae for integrated biomass production and phytoremediation of unsterilized wastewater and anaerobic digestion centrate

Bohutskyi

Liu

Nasr

et al. 2015

Appl Microbiol Biotechnol

111

View full text Add to dashboard Cite

show abstract

“…Chlorella emersonii CCAP 211-2p and Chlorella marina CCAP 211-27 fall within the Scenedesmus clade in this analysis and, in character with this phylogenetic placement, produce algaenan. Another Chlorella species, Chlorella fusca, has been reinterpreted as a unicellular Scenedesmus species (Huss et al, 1999) and is also known to produce algaenan (Burczyk et al, 1999;Derenne et al, 1992; Chlorella vacuolatus CCAP 211-8b was found to produce algaenan (Derenne et al, 1992) and has since been renamed Scenedesmus vacuolatus; it groups with all other Scenedesmus in Fig. 3.…”

Section: Phylogenetic Patterns Of Algaenan Production In the Green Algaementioning

confidence: 99%

“…Strain UTEX 1230 produced algaenan when harvested during log growth (Zleibor et al, 1988) while CCAP 211-8k did not when harvested after 30 days. The growth phase of the culture was not identified, although it was likely stationary phase (Burczyk et al, 1999). Although two different extraction methods were used in the algaenan analysis, CCAP 211-8k was subjected to the milder extraction (acid hydrolysis in 6 N HCl, but without a saponification step) and would be less likely to produce a negative result as an artifact of processing.…”

Section: Physiological Insights From Phylogenetic Assessment Of Algaementioning

confidence: 99%

Phylogenetic investigation of the aliphatic, non-hydrolyzable biopolymer algaenan, with a focus on green algae

Kodner

Summons

Knoll

2009

Organic Geochemistry

View full text Add to dashboard Cite

“…[6,7,8] Arouri et al [9,10] studied the ultrastructure and chemistry of Neoproterozoic acritarchs from Australia and suggested a dinoflagellate affinity for the acanthomorph (process-bearing) forms with a multilayered, fibrillar wall, and a chlorophycean relationship to other taxa whose walls preserve a laminated organisation that is similar to the trilamellar structure (TLS) found in some extant green microalgae. [11,12] Recently, Kazmierczak and Kremer [4] described the internal bodies of a variety of early Silurian and late Devonian acritarchs and suggested that they were spores (autospores or aplanospores). The presence of spore-like bodies in acritarchs supports earlier explanations that some of them can be affined with unicellular green algae.…”

Section: Introductionmentioning

confidence: 99%

Laser‐Raman and atomic force microscopy assessment of the chlorococcalean affinity of problematic microfossils

Kremer

Bauer

Stark

et al. 2011

J Raman Spectroscopy

View full text Add to dashboard Cite

Organic-walled microfossils of uncertain origin, classified to an informal group named acritarchs, are most commonly interpreted as the resting cysts of marine eukaryotic phytoplankton. Some acritarchs have recently been interpreted as vegetative cells of chlorococcalean green algae, based on internal bodies that have been interpreted as their asexual reproductive structures (spores). To verify this interpretation, we applied confocal Raman spectroscopy and atomic force microscopy (AFM) to study the ultrastructure and nanostructure of exceptionally preserved acritarchs with internal bodies from the early Silurian cherts (c. 430 Ma-old) of Frankenwald (Germany). Three-dimensional Raman mapping showed the spatial distribution of carbonaceous material and other minerals in the walls of the analysed internal bodies and confirmed that these structures are comparable with spores of chlorococcalean microalgae. Our findings document therefore the oldest thus far known vegetative cells of sporulating green algae. The combination of confocal Raman and AFM techniques yielded detailed information about the nanostructure and fossilisation mode of the mineralised organic walls of both the central vesicles and the enclosed spore-like bodies.Keywords: acritarch microfossils, mineralization, Silurian, Raman, AFM 2 IntroductionThe informal taxonomic category of organic-walled microfossils named acritarchs [1] has an unclear systematic affiliation. They are traditionally interpreted as the cysts of unicellular organisms. [1,2] During the Proterozoic and Paleozoic, acritarchs formed the basis of the marine food chain and played an important role in the evolution of the global ecosystem. Acritarchs were therefore the main primary producers in the ocean for a long time and, as oxygenic microorganisms, they must have not only controlled the evolution of the marine ecosystem but also influenced planetary biochemical cycles. Acritarch-like fossils are among the oldest morphological traces of life [3] , and the elucidation of their biological relationships sheds new light on the evolution of the early biosphere. It has recently been suggested that at least some acritarchs are the sporulating vegetative stages of unicellular green microalgae because they show internal bodies that can be interpreted as spores. [4] The classification of acritarchs as green algae therefore has an important bearing on the overall understanding of basic processes in the evolution of Earth's biosphere. A precise morphological and chemical characterisation of acritarch internal bodies and of their threedimensional arrangement is difficult because they are typically studied either in thin sections or after extraction from fossil-bearing rock. A detailed chemical or morphological study of microfossils usually requires physical or chemical preparation to extract the microfossils from the rock matrix, which can cause their partial or even total destruction. However, even the maceration process does not allow for extraction and study of these microfossils and their f...

show abstract

Comparison of nitrogen content amino acid composition and glucosamine content of cell walls of various chlorococcalean algae

Cited by 50 publications

References 23 publications

Bioprospecting of microalgae for integrated biomass production and phytoremediation of unsterilized wastewater and anaerobic digestion centrate

Bioprospecting of microalgae for integrated biomass production and phytoremediation of unsterilized wastewater and anaerobic digestion centrate

Phylogenetic investigation of the aliphatic, non-hydrolyzable biopolymer algaenan, with a focus on green algae

Laser‐Raman and atomic force microscopy assessment of the chlorococcalean affinity of problematic microfossils

Contact Info

Product

Resources

About