Comparison of Native and Reduced and Alkylated Human Iv Immune Serum Globulin (Isg) in Experimental H. In Fluenzae Type B Infection

Abstract

~h k antigenic heterogeneity among the-more than 70 serotypes o f enteroviruses has been the major obstacle t o rapid diagnosis o f infections due t o these agents.A common antigen detectable by immunodiagnostic techniques has n o t been found. The enteroviruses do, however, share numerous physico-chemical and microbiological characteristics, suggesting some genetic conservation may exist across a l l o f the subgroups. To t e s t t h a t hypothesis, we radioactively labeled fragments o f DNA which are complementary (cDNA) t o the genomic RNA o f type 1 poliovirus. These cDNA sequences have been cloned i n t o the pBR 325 plasmid o f E. c o l i HB101. We then infected separate plates o f LLC-MK2 tissue c-cells with type 1, type 2, and type 3 polioviruses, coxsackieviruses A9 and B 1 and echovirus 11-