Comparison of MicroRNA Profiles in Extracellular Vesicles from Small and Large Goat Follicular Fluid

Ding, Qiang; Jin, Min Kyung; Kalds, Peter; Meng, Chunhua; Wang, Huili; Zhong, Jun; Wang, Xiaolong; Chen, Yulin

doi:10.3390/ani11113190

Cited by 5 publications

(2 citation statements)

References 40 publications

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“…Currently, numerous continuing studies are elucidating the functionality and role of livestock-derived EVs in livestock industry fields [44][45][46][47]. As the first to isolate rumen derived EVs, this study showed that REVs increased pathogen resistance by regulating nematode immune genes.…”

Section: Discussionmentioning

confidence: 89%

Molecular characterization and functionality of rumen-derived extracellular vesicles using a Caenorhabditis elegans animal model

Choi¹,

Mun²,

Ryu³

et al. 2023

J Anim Sci Technol

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Section: Discussionmentioning

confidence: 89%

Molecular characterization and functionality of rumen-derived extracellular vesicles using a Caenorhabditis elegans animal model

Choi¹,

Mun²,

Ryu³

et al. 2023

J Anim Sci Technol

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“…Among the top 20 highly abundant miRNAs, 18 were commonly expressed in both groups including miR-148a and miR-99a, the top highly expressed miRNAs in the FF-EVs with no signi cant differences between the groups. Similarly, miR-148 was reported as the top expressed miRNA in FF-EVs from large and small follicles in goats [39]. In human FFs, miR-99a was speci cally enriched in the FF-EVs and considered as a regulator of the ovarian follicle developmental process to include meiosis resumption [40].…”

Section: Discussionmentioning

confidence: 94%

Extracellular vesicle-microRNAs mediated response of bovine ovaries to environmental heat stress

Gad

Joyce

Menjivar

et al. 2023

Preprint

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Background Elevated ambient temperature during the summer season is a main cause of stress in dairy and beef cows, leading to impaired reproductive function and fertility. Follicular fluid extracellular vesicles (FF-EVs) play an important role in intrafollicular cellular communication by in part mediating the deleterious effects of heat stress (HS). Here we aimed to investigate the changes in FF-EV miRNA cargoes in beef cows in response to HS during the summer (SUM) compared to the winter (WIN) season using high throughput sequencing of FF-EV-coupled miRNAs. In addition to their biological relevance, the potential mechanisms involved in the packaging and release of those miRNAs as a response to environmental HS were elucidated. Results Sequencing analysis revealed that an average of 6.6% of the EV-RNA mapped reads were annotated to bovine miRNAs. Interestingly, miR-148a, miR-99a-5p, miR-10b, and miR-143 were the top four miRNAs in both groups accounting for approximately 52 and 62% of the total miRNA sequence reads in the SUM and WIN groups, respectively. A group of 16 miRNAs was up-regulated and 8 miRNAs were down-regulated in the SUM compared to the WIN group. Five DE-miRNAs (miR-10a, miR-10b, miR-26a, let-7f, and miR-1246) were among the top 20 expressed miRNA lists. Sequence motif analysis revealed the appearance of two specific motifs in 13 out of the 16 upregulated miRNAs under HS conditions. Both motifs were found to be potentially bonded by specific RNA binding proteins including Y-box binding proteins (YBX1 and YBX2) and RBM42. Conclusion Our findings indicate that FF EV-coupled miRNA profile varies under seasonal temperature changes. These miRNAs could be a good indicator of the cellular mechanism in mediating HS response and the potential interplay between miRNA motifs and RNA binding proteins can be one of the mechanisms governing the packaging and release of miRNAs via EVs to facilitate cellular survival.

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Dynamics of extracellular vesicle-coupled microRNAs in equine follicular fluid associated with follicle selection and ovulation

Gebremedhn

Gad

Ishak

et al. 2023

Molecular Human Reproduction

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Innumerable similarities in reproductive cyclicity and hormonal alterations highlight the considerable utility of the mare to study aspects of follicular dynamics and reproductive function in view of the largely constricted, human research subjects. The bi-directional communication between the growing oocyte and the surrounding somatic cells embodies the hallmark of mammalian follicular development, partially mediated by extracellular vesicles (EVs) encapsulated with microRNAs (miRNAs) and present in the follicular fluid (FF). Here, we aimed to decipher the dynamics of the miRNAs in EVs from equine FF aspirated in vivo during different stages of follicular development, namely, predeviation (PreDev; 18–20 mm), deviation (Dev; 22–25 mm), postdeviation (PostDev; 26–29 mm), preovulatory (PreOV; 30–35 mm), and impending ovulation (IMP; ∼40 mm). Approximately 176 known miRNAs were found in all groups with 144 mutually detected among all groups. Cluster analysis exhibited 15 different expression patterns during follicular development. Among these patterns, a group of 22 miRNAs (including miR-146b-5p, miR-140, and miR-143) exhibited a sharp reduction in expression from the PreDev until the PreOV stage. Another cluster of 23 miRNAs (including miR-106b, miR-199a-5p, and miR-125a-5p) exhibited a stable expression pattern at the PreDev stage until the PostDev stage, with a significant increase at the PreOV stage followed by a significant decrease at the IMP stage. In conclusion, this study provides greater insights into the stage-specific expression dynamics of FF EV-miRNAs during equine follicular development, which may propose novel approaches to improve ART and provide new biomarkers to facilitate the assessment of ovarian pathophysiological conditions.

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Comparison of MicroRNA Profiles in Extracellular Vesicles from Small and Large Goat Follicular Fluid

Cited by 5 publications

References 40 publications

Molecular characterization and functionality of rumen-derived extracellular vesicles using a Caenorhabditis elegans animal model

Molecular characterization and functionality of rumen-derived extracellular vesicles using a Caenorhabditis elegans animal model

Extracellular vesicle-microRNAs mediated response of bovine ovaries to environmental heat stress

Dynamics of extracellular vesicle-coupled microRNAs in equine follicular fluid associated with follicle selection and ovulation

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