2011
DOI: 10.1007/s00436-011-2514-1
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Abstract: This study aimed to compare the sensitivity of five diagnostic methods commonly used for the detection of Toxoplasma gondii in tissues of naturally infected pigs. We purchased 20 heads of pigs in butcher shops in the city of Ilhéus, Bahia. Brain and tongue fragments were taken from each animal for the performance of PCR against T. gondii. The rest of these two tissues were processed and inoculated into three mice. These rodents were observed for 42 days and euthanized. We prepared slides with brain and lungs o… Show more

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Cited by 16 publications
(11 citation statements)
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“…The observed isolation rate of T. gondii from seropositive pigs (24/50, 48%) is in close agreement to 4 of 8 (50%) [30], and 20 of 40 (50%) [31], seropositive pigs from Brazil. It is lower compared with 39 of 69 (56%) bioassayed pigs from USA [32] and 16 of 22 (73%) seropositive pigs from Serbia [33].…”
Section: Discussionsupporting
confidence: 74%
“…The observed isolation rate of T. gondii from seropositive pigs (24/50, 48%) is in close agreement to 4 of 8 (50%) [30], and 20 of 40 (50%) [31], seropositive pigs from Brazil. It is lower compared with 39 of 69 (56%) bioassayed pigs from USA [32] and 16 of 22 (73%) seropositive pigs from Serbia [33].…”
Section: Discussionsupporting
confidence: 74%
“…The lack of association between seropositivity in pregnant women and behavioral habits such as handling sand, swimming or fishing, and eating fruits or raw vegetables, corroborated with previous findings 43 . Moreover, previous studies carried out in the same region with pigs 44 , cows 45 , sheep 46,47 oysters 48 , cats 22 , and dogs 23 , corroborated the frequency of toxoplasmosis found in the present study among pets of pregnant women, emphasizing the risk to public health caused by the direct ingestion of oocysts or tissue cysts present in raw or rare meat.…”
Section: Discussionsupporting
confidence: 91%
“…The systematic review process identified 41 studies, presented in 36 papers, dealing with the direct identification of T . gondii in pigs and meat thereof (details in Table 3 ) [ 12 , 31 , 33 , 36 , 37 , 39 , 40 , 45 69 ]. A univariable meta-regression was performed considering, as moderators, publication year, geographic area, analytical technique, farming system, sample type and sampling location.…”
Section: Resultsmentioning
confidence: 99%