Comparison of lipid deposition of intramuscular preadipocytes in Tan sheep co‐cultured with satellite cells or alone

Abstract

The purpose of this study was to investigate the effect of the skeletal muscle satellite cells (SMSCs) on the lipid deposition of the intramuscular preadipocytes (IMPs) in a co‐culture system of the Tan sheep cells. The SMSCs and IMPs from Tan sheep were separated and cultured. After the two kinds of cells were separated and cultured, they were inoculated onto a transwell cell chamber co‐culture plate for co‐cultivation. When the cell density reached more than 90%, the cells were induced to differentiate. After the induction of the SMSCs differentiation for 8 days, the level of the IMPs differentiation and the expression levels of the differentiation marker genes and the key enzymes of the lipid metabolism were assessed. The results showed that the number and area of the lipid droplets in the IMPs in the co‐culture system were significantly reduced compared to those in the IMPs culture alone (p < 0.05). Meanwhile, the expression levels of the PPARγ, c/EBPα, ACC, FAS mRNA in the IMPs were significantly decreased (p < 0.05); the expression level of aP2 mRNA was decreased, but the difference was not significant (p > 0.05).These findings indicate that the SMSCs of the Tan sheep in the co‐culture system inhibited the lipid deposition by the IMPs.

The Overlooked Transformation Mechanisms of VLCFAs: Peroxisomal β-Oxidation

“…Similarly, as milk-producing ruminants, goats and sheep are also regulated by PPARγ. Many studies indicate that PPARγ is involved in adipocyte differentiation and adipogenesis in sheep/goats [59][60][61][62][63], though, in addition to fat regulation, PPARγ was previously thought to be involved in the regulation of hormones in goats and sheep [64,65]. However, there has been some relevant research in recent years.…”

Using Vertebrate Stem and Progenitor Cells for Cellular Agriculture, State-of-the-Art, Challenges, and Future Perspectives

“…On the other hand, the results of a study done by Chu et al have shown that during cocultivation C2C12 cells inhibit the proliferation and differentiation of 3T3-L1 pre-adipocytes by suppressing glucocorticoid receptor (GR) gene expression [139]. A recently published study with Tan sheep cells showed a decrease in the number of lipid droplets in the intramuscular preadipocytes (IMPs) that were in a co-culture system with skeletal muscle SCs [140].…”