Comparison of IS6110 and ‘short fragment’ devR (Rv3133c) gene targets with phenotypic methods for diagnosis of Mycobacterium tuberculosis

Sahni, A.K.; Singh, Sukhdev; Kumar, Anil; Khan, Inam Danish

doi:10.1016/j.mjafi.2013.02.003

Cited by 3 publications

(2 citation statements)

References 12 publications

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“…devR encodes the cytoplasmic response regulator DevR , which works together with the membrane-bound sensor kinase DevS to form DevRS . During latent infection in the host, DevRS is responsible for adaptation and dormancy [ 159 ]. Despite reports that shorter fragments of the devR gene significantly increased the sensitivity of TB diagnosis [ 160 ], the sensitivity of devR PCR assay is lower than that of MPB64 and IS6110 [ 161 ].…”

Section: Molecular Diagnosismentioning

confidence: 99%

Mycobacterium tuberculosis complex in wildlife: Review of current applications of antemortem and postmortem diagnosis

et al. 2020

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Tuberculosis (TB) is a chronic inflammatory and zoonotic disease caused by Mycobacterium tuberculosis complex (MTBC) members, which affects various domestic animals, wildlife, and humans. Some wild animals serve as reservoir hosts in the transmission and epidemiology of the disease. Therefore, the monitoring and surveillance of both wild and domestic hosts are critical for prevention and control strategies. For TB diagnosis, the single intradermal tuberculin test or the single comparative intradermal tuberculin test, and the gamma-interferon test, which is regarded as an ancillary test, are used. Postmortem examination can identify granulomatous lesions compatible with a diagnosis of TB . In contrast, smears of the lesions can be stained for acid-fast bacilli, and samples of the affected organs can be subjected to histopathological analyses. Culture is the gold standard test for isolating mycobacterial bacilli because it has high sensitivity and specificity compared with other methods. Serology for antibody detection allows the testing of many samples simply, rapidly, and inexpensively, and the protocol can be standardized in different laboratories. Molecular biological analyses are also applicable to trace the epidemiology of the disease. In conclusion, reviewing the various techniques used in MTBC diagnosis can help establish guidelines for researchers when choosing a particular diagnostic method depending on the situation at hand, be it disease outbreaks in wildlife or for epidemiological studies. This is because a good understanding of various diagnostic techniques will aid in monitoring and managing emerging pandemic threats of infectious diseases from wildlife and also preventing the potential spread of zoonotic TB to livestock and humans. This review aimed to provide up-to-date information on different techniques used for diagnosing TB at the interfaces between wildlife, livestock, and humans.

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Section: Molecular Diagnosismentioning

confidence: 99%

Mycobacterium tuberculosis complex in wildlife: Review of current applications of antemortem and postmortem diagnosis

et al. 2020

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“…21,31,32 With the Middle East catering to 10 million annual pilgrims and otherwise being a global transport hub, travel-related infections and public health emergencies need to be assessed, detected, and answered with robust health systems under a One-Health approach in consonance with international health regulations. 3,5,13,33,34 Quarantine, syndromic surveillance, and cluster investigation for endemic infectious diseases including dengue and Rift Valley fever can be feasible primary tools for mass gatherings during Hajj. 35,36 The prohibition of pilgrims from Liberia, Guinea, and Sierra Leone led to zero Ebola cases during Hajj 2015.…”

Section: Incident Reporting System For Indian Hajj Pilgrimsmentioning

confidence: 99%

Indian Medical Mission at Hajj-2016: Mass-Gathering Medicine Perspectives, Challenges, and Opportunities in a Mission Posture

Khan

et al. 2017

Int J Travel Med Glob Health

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IntroductionHajj is an annual mass gathering of over 3.5 million pilgrims from 200 countries who congregate in densities of 9 people/ m2 and endure strenuous rituals, compromised living standards, and a harsh desert climate in an alien ethnocultural and sociolinguist milieu for spiritual enlightenment. Mass gathering medicine at Hajj is challenged by issues of healthcare accessibility, infection control, on-site treatment, referral, evacuation, and response to disasters and public health emergencies in addition to challenges to providing support such as shelter, food and water, transportation, mass communication, interpersonal communication, sanitation, overcrowding, and human security. 1,2Hajj has historically experienced high morbidity and mortality owing to pre-existing comorbidities, outbreaks, accidents, and disasters such as such as stampedes, fires, AbstractIntroduction: Hajj is an annual mass gathering of over 3.5 million pilgrims congregating from 200 countries in the desert climate of Saudi Arabia. Mass gathering medicine at Hajj is challenged by issues of healthcare accessibility, infection control, on-site treatment, referral, evacuation, and response to disasters and public health emergencies. The Indian Medical Mission at Hajj 2016 established, operated, and coordinated a strategic network of mass gathering medical operations, the proceeds of which are discussed herein. Methods:The mission was designed to provide holistic health security through health intelligence for pre-existing chronic diseases, epidemic intelligence for endemic and exotic diseases, public-health and disaster-health preparedness, and tiered healthcare through mobile medical task forces, static clinics, tent clinics, secondary care hospitals, and evacuation capabilities. Results: Primary care, secondary care, and tertiary care treated 374 475, 930, and 523 patients, respectively. Patients exhibited limited compliance with pre-instituted treatments and precautionary protocols. Respiratory and gastrointestinal infections, cardiorespiratory, trauma, and heat illnesses were seen. Epidemic intelligence revealed an outbreak of food poisoning. Respiratory infections were reported by 90% of the healthcare personnel. Surge capacity was overwhelmed with patient throughput and ambulance transfers. Crude unadjusted mortality was 11.99/10 000. Conclusion:The Indian Medical Mission at Hajj 2016 yielded solutions to the challenges faced during the 2016 Hajj pilgrimage. The mission posture of the Indian Medical Mission in Hajj presents a modus operandi for handling crisis scenarios in mass gathering. The situational analysis of the Hajj health mission calls for dynamic interventions in preparedness, clientele, and health systems.

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DNA markers for tuberculosis diagnosis

et al. 2018

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Tuberculosis (TB), caused by Mycobacterium tuberculosis complex (MTBC), is an infectious disease with more than 10.4 million cases and 1.7 million deaths reported worldwide in 2016. The classical methods for detection and differentiation of mycobacteria are: acid-fast microscopy (Ziehl-Neelsen staining), culture, and biochemical methods. However, the microbial phenotypic characterization is timeconsuming and laborious. Thus, fast, easy, and sensitive nucleic acid amplification tests

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Comparison of IS6110 and ‘short fragment’ devR (Rv3133c) gene targets with phenotypic methods for diagnosis of Mycobacterium tuberculosis

Cited by 3 publications

References 12 publications

Mycobacterium tuberculosis complex in wildlife: Review of current applications of antemortem and postmortem diagnosis

Mycobacterium tuberculosis complex in wildlife: Review of current applications of antemortem and postmortem diagnosis

Indian Medical Mission at Hajj-2016: Mass-Gathering Medicine Perspectives, Challenges, and Opportunities in a Mission Posture

DNA markers for tuberculosis diagnosis

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