Comparison of intestinal anaphylactic reactions in sensitized mice challenged with untreated bovine milk and homogenized bovine milk

Abstract

Outbred NMRI mice were sensitized for high IgE production either by subcutaneous injections of low doses of untreated bovine milk or homogenized bovine milk in combination with intraperitoneal injections of Freund's Complete Adjuvant or by oral administration of untreated or homogenized bovine milk without adjuvant. When analysed in murine passive cutaneous anaphylaxis test both types of milk resulted in production of reaginic antibodies against bovine milk proteins when given subcutaneously. When given orally, homogenized milk resulted in reagin production in 10 out of 14 mice, whereas untreated milk resulted in reagin production in only one out of 12 mice. The sensitized mice, and control mice, were orally challenged with either untreated milk, homogenized milk or 0.9% NaCl. Examination of the intestines 40 min after oral administration revealed that homogenized milk, contrary to untreated milk or 0.9% NaCl, resulted in a large increase in the mass of the proximal gut segment of mice sensitized orally with homogenized milk compared with control mice orally challenged with saline (P less than 0.001), and only mice both sensitized and challenged orally with homogenized milk showed degranulation of mast cells in the intestinal wall. By contrast, subcutaneously sensitized mice or mice sensitized orally with untreated bovine milk showed no significant intestinal reaction upon oral challenge with either homogenized or untreated bovine milk. These observations may indicate that the route of sensitization is of great importance when intestinal reactions are to be studied, and that homogenization of bovine milk may render the milk more aggressive with respect to its ability to induce intestinal reactions. The study indicates that mice may be an attractive experimental animal model for mimicking the intestinal anaphylactic reactions of cow milk-allergic humans.

Epicutaneous Immunotherapy Using a New Epicutaneous Delivery System in Mice Sensitized to Peanuts

“…Poulsen et al [24] demonstrated very early on that intestinal anaphylaxis after an oral challenge is associated with the release of histamine and other mediators leading to acute systemic symptoms. We thus performed a histamine provocation test, as already described by Li et al [25,26,27].…”

Can farm milk consumption prevent allergic diseases?

“…Allergy‐related health effects associated with homogenization have been discussed in the context of milk allergy (reviewed in [15]). In animal models there is some evidence that homogenization favours milk allergy [16], but these findings have not been confirmed in clinical studies [15]. No studies exist so far examining a potential effect of homogenization on the development of asthma or allergic diseases other than milk allergy.…”

Sensitizing Capacity and Residual Allergenicity of Hydrolyzed Cow’s Milk Formulae: Results from a Murine Model

“…Homogenization of cow’s milk is also a factor which improves the induction of sensitization in a murine model [29]. …”