Comparison of interleukin-12 with lung cancer and malignant lymphoma undergoing autologous peripheral blood stem cell transplantation

Yoshimura, Chie; Nomura, Shosaku; Kanazawa, Shigenori; Kuwana, Midori; Yamaguchi, Kazuyuki; Fukuhara, Shirou

doi:10.1007/s004320100270

Cited by 3 publications

(2 citation statements)

References 30 publications

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“…Among many influential factors, the cytokine microenvironment, in which Th0 cells contact APCs, is important in regulating Th cell differentiation. For example, IL-12 and IFN-γ promote Th0 cells to differentiate to Th1 cells [34] , [35] , and IL-4 promotes Th0 cells to differentiate to Th2 cells.…”

Section: Discussionmentioning

confidence: 99%

Activation of Lymphocytes Induced by Bronchial Epithelial Cells with Prolonged RSV Infection

2011

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Respiratory syncytial virus (RSV) preferentially infects airway epithelial cells,which might be responsible for susceptibility to asthma; however, the underlying mechanism is not clear. This study determined the activation of lymphocytes and drift of helper T (Th) subsets induced by RSV-infected human bronchial epithelial cells (HBECs) in vitro. HBECs had prolonged infection with RSV, and lymphocytes isolated from human peripheral blood were co-cultured with RSV-infected HBECs. Four groups were established, as follows: lymphocytes (group L); lymphocytes infected with RSV (group RL); co-culture of lymphocytes with non-infected HBECs (group HL); and co-culture of lymphocytes with infected HBECs (group HRL). After co-culture with HBECs for 24 hours, lymphocytes were collected and the following were determined in the 4 groups: cell cycle status; apoptosis rate; and concentrations of IL-4, IFN-γ, and IL-17 in the supernatants. Cell cycle analysis for lymphocytes showed a significant increase in S phase cells, a decrease in G1 phase cells, and a higher apoptosis rate in group HRL compared with the other three groups. In group HRL, the levels of IL-4, IFN-γ, and IL-17 in supernatants were also higher than the other three groups. For further study, lymphocytes were individually treated with supernatants from non-infected and RSV-infected HBECs for 24 h. We showed that supernatants from RSV-infected HBECs induced the differentiation of Th2 and Th17 subsets, and suppressed the differentiation of Treg subsets. Our results showed that HBECs with prolonged RSV infection can induce lymphocyte proliferation and apoptosis, and enhance the release of cytokines by lymphocytes. Moreover, subset drift might be caused by RSV-infected HBECs.

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Section: Discussionmentioning

confidence: 99%

Activation of Lymphocytes Induced by Bronchial Epithelial Cells with Prolonged RSV Infection

2011

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“…IL-12 is a critical cytokine co-operating with the CD28 costimulator and inducing T-cell proliferation and cytokine secretion [7,13,31]. CD28 promotes the synthesis of IFN-c protein, enhancing the stability of IFN-c mRNA, and facilitates the expression of the IL-12 receptor [24].…”

Section: Discussionmentioning

confidence: 99%

Differential effects of propofol and isoflurane on the activation of T‐helper cells in lung cancer patients

Ren

Meng

et al. 2010

Anaesthesia

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SummaryIt is suggested that activation and differentiation of T-helper cells are required for peri-operative anti-tumor and anti-infection immunity. The present study aimed to evaluate whether propofol stimulates the activation and differentiation of these cells in patients undergoing pulmonary lobectomy for non-small-cell lung cancer. Thirty patients were randomly allocated to receive propofol or isoflurane throughout surgery. The CD4 + CD28 + percentage (p < 0.0001) and the ratio of interferon-c:interleukin-4 (p = 0.001) all increased with propofol but showed no change with isoflurane. In contrast, cortisol increased with isoflurane (p < 0.0001) but not with propofol over time (p = 0.06). We conclude that propofol promotes activation and differentiation of peripheral T-helper cells.

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Interleukin-12 prevents colorectal cancer liver metastases in mice

Shen

Li²,

Cai³

et al. 2013

OTT

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Interleukin (IL)-12 has emerged to be a prospective molecule for antitumor therapies with many types of cancers. Here we examine the effect of IL-12 treatment in preventing the colorectal cancer liver metastasis in a mice model. At different doses, we found that IL-12 treatment decreased the formation of liver metastasis sites and the percentage of metastasis volume in the liver. Additionally, this treatment leads to improved survival function of mice after tumor cell transplantation. We believe that IL-12 based therapy provided a novel treatment to colorectal cancer patients suffering from liver metastasis.

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Comparison of interleukin-12 with lung cancer and malignant lymphoma undergoing autologous peripheral blood stem cell transplantation

Abstract: Our findings suggest that PBSC in lung cancer are potent mediators of anticancer activity and that they might play an immunotherapeutic role against autologous malignant cells.

Cited by 3 publications

References 30 publications

Activation of Lymphocytes Induced by Bronchial Epithelial Cells with Prolonged RSV Infection

Activation of Lymphocytes Induced by Bronchial Epithelial Cells with Prolonged RSV Infection

Differential effects of propofol and isoflurane on the activation of T‐helper cells in lung cancer patients

Interleukin-12 prevents colorectal cancer liver metastases in mice

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