Comparison of global responses to mild deficiency and excess copper levels in Arabidopsis seedlings

Andrés-Colás, Nuria; Perea-García, Ana; Mayo, Sonia; Garcia‐Molina, Antoni; Dorcey, Eavan; Rodrı́guez-Navarro, Susana; Pérez‐Amador, Miguel A.; Puig, Sergi; Peñarrubia, Lola

doi:10.1039/c3mt00025g

Cited by 29 publications

(33 citation statements)

References 62 publications

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“…Thereto, 5-day-old wild type seedlings grown on low but Cu sufficient medium (½ MS supplemented with CuSO 4 0.5 μM [31]) were incubated for 3 h on liquid ½ MS with tunicamycin [32], an inhibitor of N-linked protein glycosylation, or dithiothreitol (DTT), disrupting disulphide bond formation. The presence of ER stress in our experimental conditions was confirmed by raised BiP1,2 and CRT1 transcript levels in comparison to controls (Figure 4).…”

Section: Resultsmentioning

confidence: 99%

Functional characterisation of Arabidopsis SPL7 conserved protein domains suggests novel regulatory mechanisms in the Cu deficiency response

2014

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BackgroundThe Arabidopsis SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE (SPL) transcription factor SPL7 reprograms cellular gene expression to adapt plant growth and cellular metabolism to copper (Cu) limited culture conditions. Plant cells require Cu to maintain essential processes, such as photosynthesis, scavenging reactive oxygen species, cell wall lignification and hormone sensing. More specifically, SPL7 activity promotes a high-affinity Cu-uptake system and optimizes Cu (re-)distribution to essential Cu-proteins by means of specific miRNAs targeting mRNA transcripts for those dispensable. However, the functional mechanism underlying SPL7 activation is still to be elucidated. As SPL7 transcript levels are largely non-responsive to Cu availability, post-translational modification seems an obvious possibility. Previously, it was reported that the SPL7 SBP domain does not bind to DNA in vitro in the presence of Cu ions and that SPL7 interacts with a kin17 domain protein to raise SPL7-target gene expression upon Cu deprivation. Here we report how additional conserved SPL7 protein domains may contribute to the Cu deficiency response in Arabidopsis.ResultsCytological and biochemical approaches confirmed an operative transmembrane domain (TMD) and uncovered a dual localisation of SPL7 between the nucleus and an endomembrane system, most likely the endoplasmic reticulum (ER). This new perspective unveiled a possible link between Cu deficit and ER stress, a metabolic dysfunction found capable of inducing SPL7 targets in an SPL7-dependent manner. Moreover, in vivo protein-protein interaction assays revealed that SPL7 is able to homodimerize, probably mediated by the IRPGC domain. These observations, in combination with the constitutive activation of SPL7 targets, when ectopically expressing the N-terminal part of SPL7 including the SBP domain, shed some light on the mechanisms governing SPL7 function.ConclusionsHere, we propose a revised model of SPL7 activation and regulation. According to our results, SPL7 would be initially located to endomembranes and activated during ER stress as a result of Cu deficiency. Furthermore, we added the SPL7 dimerization in the presence of Cu ions as an additional regulatory mechanism to modulate the Cu deficiency response.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-014-0231-5) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Functional characterisation of Arabidopsis SPL7 conserved protein domains suggests novel regulatory mechanisms in the Cu deficiency response

2014

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“…Due to the decrease in Cu availability there is no proper functioning of Cu/Zn SOD, causing further rise of oxidative stress (Marschner, 1995;Küpper and Kroneck, 2005). Alteration of the functioning of electron transport chains in both the mitochondria and chloroplasts through the malfunctioning of the Cu proteins such as cytochrome c oxidase and plastocyanin where reported as a response of Cu deficiency (Andrés-Colás et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Effects of nanomolar copper on water plants—Comparison of biochemical and biophysical mechanisms of deficiency and sublethal toxicity under environmentally relevant conditions

et al. 2013

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“…Insufficient supply of Cu can result in abnormal plant growth and development, both of which have been widely reported in previous studies (Yu and Rengel, 1999;Andrés-Colás et al, 2013). However, an excess of Cu, a serious environmental problem arising and spreading together with industrialization and the improper management of fertilizers and irrigation in agriculture in the past decades, can also adversely affect physiological processes and biochemical reactions in organisms and endanger the environment and, more particularly, human health (Hirayama et al, 2012;Wen et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Alleviation of photosynthetic inhibition in copper-stressed tomatoes through rebalance of ion content by exogenous nitric oxide

Wang¹,

Yang²,

Ren³

et al. 2015

Turk J Bot

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Copper (Cu) is an essential element that plays a very important role in plant growth and development. Its accumulation in soil is now considered a serious environmental problem endangering agricultural production and human health. In this study, the influence of exogenous nitric oxide (NO) on photosynthesis, Cu, magnesium (Mg), and iron (Fe) contents was studied in tomato seedlings under Cu stress grown in hydroponic culture. The results showed that the addition of sodium nitroprusside (SNP), a donor of exogenous NO, significantly alleviated photosynthetic inhibition in Cu-stressed tomatoes, including the recovery of photosynthetic pigment content and chlorophyll a fluorescence and the improvement of net photosynthetic rate, stomatal conductance, and transpiration rate. Application of exogenous NO by SNP reduced the excessive accumulation of Cu and recovered Mg and Fe contents in Cu-stressed tomatoes. This positive involvement of exogenous NO in tomato tolerance to Cu stress was further confirmed by addition of 2-(4-carboxyphenyl)-4,4,5,5tetramethylimidazoline-1-oxyl-3-oxide (cPTIO), a specific NO scavenger, which resulted in further reduction of photosynthesis and more severe disturbance of ion homeostasis in Cu-stressed seedlings. These results indicate that rebalance of ion content was involved in alleviation of photosynthesis inhibition in Cu-stressed tomatoes by exogenous NO.

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Comparison of global responses to mild deficiency and excess copper levels in Arabidopsis seedlings

Cited by 29 publications

References 62 publications

Functional characterisation of Arabidopsis SPL7 conserved protein domains suggests novel regulatory mechanisms in the Cu deficiency response

Functional characterisation of Arabidopsis SPL7 conserved protein domains suggests novel regulatory mechanisms in the Cu deficiency response

Effects of nanomolar copper on water plants—Comparison of biochemical and biophysical mechanisms of deficiency and sublethal toxicity under environmentally relevant conditions

Alleviation of photosynthetic inhibition in copper-stressed tomatoes through rebalance of ion content by exogenous nitric oxide

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