Comparison of Fluorescence Immunochromatography and HPLC for the Trace Analysis of Algal Toxins

Lateral flow based immunobiosensors for detection of food contaminants

“…Moreover, Pyo et al (Pyo, Choi et al 2004) applied a lateral flow device (LFD) for measuring MC and the assay had the LOD of 200 pg mL −1 and was able to detect all the main MCs (Pyo, Choi et al 2004). …”

“…However, the better sensitivity could be the result of the fact that the fluorescent LFD was provided with instrumental detection whereas results from GNP-based LFD were evaluated visually, which reduces detectability (Pyo, Choi et al 2004). …”

Chromatographic and capillary electrophoretic determination of microcystins

“…Double extraction with 75% methanol was also employed for homogenates of algae and fish tissue [37]. In another procedure, extraction of cells with 25% methanol [27] or 5% acetic acid [16] is assisted by sonication, whereas in another one proportion 50% methanol and 50% acetic acid was found as the best [47]. The use of acetic acid instead of methanol is advantageous because of more efficient elimination of proteins and possibility of direct use of extracts for preconcentration on C18 sorbents.…”

“…On such sorbents MC were extracted from aqueous solutions and then eluted with methanol [25,27,40,47,54,56,61]. In combined procedures for the determination of MC in waters, the samples were first screened using protein phosphatase assays and positives were concentrated using SPE on Bakerbond C18 Polarplus sorbent, which was selected as optimum of seven different sorbents examined [44].…”

“…The HPLC analysis revealed the presence of compounds with a similar absorbance spectrum to MC‐LR, showing that identification based solely on UV absorbance is not sufficient. The HPLC/UV method was also compared with an immunochromatographic assay with fluorimetric detection based on the application of mAbs against MC 47. The developed assay‐strip was in‐house fabricated and the minimum detection level of 50 ng/L (50 ppt) was shown.…”