Comparison of flavonoid from clove leaf oil cytotoxic activities with doxorubicin and cisplatin on liver cancer cell culture

Abstract

In the development of new drugs as candidates for cancer therapy agents, cytotoxic testing is needed as an initial screening to determine the effect of a natural substance in inhibiting tumour cell growth. One of the active compounds of flavonoid derivatives that are being studied as anticancer is a compound derived from clove leaf oil. The purpose of this study was to determine the cytotoxic activity of flavonoid compounds from clove leaf oil on liver cancer cell culture and make comparisons with standard drugs for cancer therapy. Examination of cytotoxic activity was carried out on HepG2 cell line culture used MTT method. The absorbance of each well was measured with spectrophotometer at a wavelength of 595 nm. The absorbance results were calculated to create a cytotoxic curve. Based on the cytotoxic curve, IC50 values of flavonoid compounds, doxorubicin and cisplatin were 50.62μg/mL, 20.25μg/mL, 15.42μg/mL respectively. Those showed that flavonoid compounds from clove leaf oil have strong anti-cancer activity (IC50<100μg/mL) against liver cancer cells. It was concluded that flavonoid compounds isolated from clove leaf oil were shown to have anticancer activity on liver cancer cell and thus could be used as a new candidate for liver cancer therapy agents.