Comparison of fermentation properties and specific enzyme activities of free and calcium-alginate-entrapped Saccharomyces cerevisiae

Abstract

Physiological properties have been determined for calcium-alginate-entrapped Saccharomyces cerevisiae in comparison to cells in suspension under identical culture conditions. Cells grown in the form of microcolonies in the alginate beads showed faster glucose uptake and ethanol productivity with simultaneously decreased product and cell yields. Increased specific hexokinase and phosphofructokinase activities could be determined in these cells. Immobilized single cells showed only slightly enhanced glucose turnover and no higher specific hexokinase activity. The significant alterations in physiology are apparently connected with growth of the cells in aggregates.

Relationship between fermentation capability and fatty acid composition of free and immobilized Saccharomyces cerevisiae

“…In previous works enhanced glucose transport into the cell and both higher levels and increased stability of key glycolytic enzymes were demonstrated in immobilized S. cerevisiae (Galazzo and Bailey 1990;Hilge-Rotmann and Rehm 1990). These results are in accordance with the improved fermentative ability at high sugar concentrations caused by altered fatty acid composition of immobilized S. cerevisiae that we obtained in this study.…”

“…Recent studies on free and immobilized S. cerevisiae revealed a greater amount of macromolecular cell components and intracellular metabolites (Galazzo and Bailey 1989;Hilge and Rehm 1989) as well as faster glucose turnover, indications of altered metabolic regulation and enhanced Offprint requests to: H.-J. Rehm activity of glycolysis key enzymes in entrapped cells (Doran and Bailey 1987;Galazzo and Bailey 1990;Hilge-Rotmann and Rehm 1990).…”

Growth kinetics of gel-immobilized yeast cells studied by on-line microscopy

“…When a gel system is inoculated with a low cell concentration, each growing active cell will give rise to a microbial colony (Bailliez et al 1985; Marin-IniCsta et al 1988; Gosmann and Rehm 1988;Hilge-Rotmann and Rehm 1990). We observed the morphology of small yeast microcolonies in the microscope reactor (Fig.…”

Influence of growth conditions on physicochemical surface characteristics of two gramnegative and two grampositive bacteria

“…Hilge-Rotmann and Rehm, 1990;Navarro and Durand, 1977). Doran and Bailey (1986) characterized the influence of microbial immobilization on metabolic activity.…”