Comparison of Extended Virulence Genotypes for Bacteria Isolated From Pediatric Patients With Urosepsis, Acute Pyelonephritis, and Acute Lobar Nephronia

Abstract

Our findings suggested that urosepsis isolates carry more virulence factors and are likely more urovirulent compared with cystitis, APN, and ALN isolates.

Factors Associated With Bacteremia in Young Infants With Urinary Tract Infection

“…The same virulence factors responsible for the higher rate of bacteremia associated with certain E. coli strains were found responsible for damage to human renal epithelial cells in cell culture. [27][28][29][30][31][32][33][34] Structural abnormalities and vesico-uretheral reflux, a potential cause of increased blood creatinine, were found in the same frequency in the bacteremic and nonbacteremic episodes.…”

Escherichia coli Bacteremia in Children

“…Bacterial characteristics are reported in Table 3. Virulence score was higher in ≤3 month of age children [median (min-max)= 15 (5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18) vs. 10 (1-19), P = 0.02], with 3 virulence factors being more frequently observed: neuC (K1 antigen, 55.8% vs. 26.8%, P = 0.009), irp2 (100% vs. 87.8%, P = 0.02) and fyuA (100% vs. 87.8%, P = 0.02).…”

“…A higher content in virulence factors was thus reported in strains causing urosepsis when compared with strains causing urinary tract infections without bacteremia. 16 However, there is a lack of prospective studies taking into account both host and bacterial characteristics versus the clinical outcome of E. coli bacteremia in children.…”

“…[15][16][17] They include adhesins, iron uptake systems, protectins and toxins, with differences according to the portal of entry. A higher content in virulence factors was thus reported in strains causing urosepsis when compared with strains causing urinary tract infections without bacteremia.…”

Studies on Clinical Characteristics, Urovirulence Factor and Host Susceptibility Gene in Pediatric Acute Lobar Nephronia

“…Furthermore, some virulence factors are more prevalent in specific urinary tract infectious diseases, thus offering insights into future vaccine development (Jantunen et al 2000;Ruiz et al 2002;Tseng et al 2002). We sought to further elucidate the roles of E. coli virulence factors in the development of urosepsis and two other severe renal parenchymal infectious diseases, APN and ALN, in (Cheng et al, 2010b). Twenty-five virulence factors were analyzed, including genes associated with fimbrial and nonfimbrial adhesins (papAH,papC,papEF,papG I,papG II,papG III,sfaS,focG,afa,bmaE,gafD,nfaE,fimH),toxins (hlyA,cnf1,cdtB), siderophores (fyuA, iutA), capsule synthesis (kpsMT II, kpsMT III), invasion of brain endothelium (ibeA), serumresistance (traT), markers for virulence-associated E. coli serogroup O4 (rfc) and colcin V plasmids (cvaC), and the coding region of PAI from the uropathogenic strain CFT073 (PAI) (Johnson and Stell 2000;Jantunen et al 2000;Tseng et al 2002;Johnson and Kuskowski et al 2005;Cheng et al 2007).…”