Comparison of exact matching and calibration curve quantification methods for glucose in human serum GC-IDMS analysis and their application for the development of certified reference materials

Barrabin, Juliana; Garrido, Bruno Carius; Machado, Gabriella A.; Wollinger, Wagner; Freitas, R.F.; Feliciano, Ana Carolina S.; Cruz, Márcia N. S. de la

doi:10.1007/s00769-015-1168-6

Cited by 3 publications

(7 citation statements)

References 25 publications

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“…Further details on the calculation approach used can be found in previous study reported by our group. 24…”

Section: Measurement Uncertainty Estimationmentioning

confidence: 99%

“…Because the physicochemical properties of the isotopologue are extremely similar to those of the analyte, we can assume that most of these effects will occur in a similar way to the analyte, thus leading to almost no variation in the analyte/internal standard ratio. 24 Thus, the main goal of this study was to develop and validate methods for the quantication of benzoic acid in orange juice and similar products by mass spectrometry using FIA and esterication followed by HS-GC to evaluate their performance as fast methods for quantication.…”

Section: Introductionmentioning

confidence: 99%

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Quantification of benzoic acid in beverages: the evaluation and validation of direct measurement techniques using mass spectrometry

2016

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“…Further details on the calculation approach used can be found in previous study reported by our group. 24…”

Section: Measurement Uncertainty Estimationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Quantification of benzoic acid in beverages: the evaluation and validation of direct measurement techniques using mass spectrometry

2016

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“…This technique has been employed in various areas of analytical chemistry, providing high accurate reference measurements [9,10]. An exact correlation between the calibration solutions and the sample solution is expected for IDMS method and its traceability can be demonstrated through a deep investigation of all sources of bias [11].…”

Section: Introductionmentioning

confidence: 99%

“…The isotopic dilution technique applied to organic compounds can be used in two different approaches: one using a calibration curve with a native standard and its isotopically labeled internal standard, and the other called exact matching IDMS, performed by comparing the area ratio of the analyte and the sample isotopically labeled standard with the same ratio from a synthetic sample (prepared in the same analytical run) [9].…”

Section: Introductionmentioning

confidence: 99%

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Characterization of calibration solution certified reference material for polycyclic aromatic hydrocarbons by exact matching isotopic dilution mass spectrometry: a case study from the Brazilian Metrology Institute

Guimarães,

Do Rego,

Rodrigues

et al. 2024

Metrologia

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Recertification is a common practice in National Metrology Institutes (NMIs) whenever advances are made in certified reference material (CRM) characterization procedures, which play a key role in metrological traceability. This article compares measurements of 16 priority Polycyclic Aromatic Hydrocarbons (PAH) in toluene by gas chromatography isotopic dilution mass spectrometry (GC-IDMS) using either an calibration curve or the exact matching approach. The multi-point IDMS calibration curve was used on the original CRM certification and the exact matching approach was carried out on its recertification. The measurement uncertainties by exact matching approach (0.3 % to 1.0 %) were approximately 5 times smaller than those obtained using the calibration curve (1.5 % to 4.4 %) for all PAHs, reducing the uncertainty component due to characterization of PAH CRM. This new characterization study added reliability, increased accuracy and reduced the measurement uncertainty of the Inmetro CRM, with metrological traceability to the International System of Units (SI). The exact-matching isotope dilution is an important technique to value assign organic compounds in CRMs, an action that consolidates the chemical metrology in the Brazilian Metrology Institute.

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Certification of a Multicomponent Reference Material from Natural Gas Mixture by Gravimetry and Dual GC-FID/TCD System

Shehata,

AlAskar,

AlYami

et al. 2023

GSC

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Natural gas (NG) is one of the most important sources of energy for industrial and domestic consumption in the present era because it is cheap and free from sulfur impurities. Therefore, accurate and precise measurement of its composition is of fundamental importance for trade reasons. To improve the quality of NG gas measurements, certified reference materials (CRMs) should be used for calibration of measuring equipment in order to ensure the traceability of the measurement results to the SI units. For the traceability purpose, a multicomponent natural gas mixture was prepared gravimetrically as a reference material according to ISO 6142 from pure helium, hydrogen, n-pentane, i-pentane, n-butane, i-butane, propane, ethane, hexane, methane and nitrogen. The preparation was done in two dilution steps in 5 L aluminum cylinders. The calculated mole fractions and associated uncertainties of natural gas components were verified by a dual GC-FID/TCD system in accordance with ISO 6143 calibrated by a series of primary gas mixtures (CRMs) produced by an NMI. The results obtained by gravimetry and by GC measurements have been checked for compatibility as required by ISO 6142 and were found in very good agreement. Details of the preparation and calculation of the mole fractions and uncertainties of all gas components are explained in this article.

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Comparison of exact matching and calibration curve quantification methods for glucose in human serum GC-IDMS analysis and their application for the development of certified reference materials

Cited by 3 publications

References 25 publications

Quantification of benzoic acid in beverages: the evaluation and validation of direct measurement techniques using mass spectrometry

Quantification of benzoic acid in beverages: the evaluation and validation of direct measurement techniques using mass spectrometry

Characterization of calibration solution certified reference material for polycyclic aromatic hydrocarbons by exact matching isotopic dilution mass spectrometry: a case study from the Brazilian Metrology Institute

Certification of a Multicomponent Reference Material from Natural Gas Mixture by Gravimetry and Dual GC-FID/TCD System

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