Comparison of effusion cell block and biopsy immunohistochemistry in mesothelial hyperplasia, mesothelioma, and carcinoma in dogs

Milne, Elspeth; Piviani, Martina; Hodgkiss-Geere, Hannah; Piccinelli, Chiara; Cheeseman, Michael; Cazzini, Paola; Ressel, Lorenzo; Marcos, Ricardo; Marrinhas, Carla; Santos, Marta; Thomas, Emily K.; Drummond, D.; Pereira, Yolanda Martínez

doi:10.1111/vcp.13002

Cited by 7 publications

(17 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Other comparative techniques include gel, cell block, and needle rinse cell block. [1][2][3][4] The resultant tissue architecture and arrangement were very similar to full-thickness biopsy and with the presence of osteoid supporting the diagnosis of osteosarcoma. The interpretation of the initial routine cytology was osteosarcoma or less likely plasma cell tumor.…”

mentioning

confidence: 61%

“…With direct aspiration and formalin fixation, similar architecture was maintained when compared to histopathology. Other comparative techniques include gel, cell block, and needle rinse cell block 1–4 . The resultant tissue architecture and arrangement were very similar to full‐thickness biopsy and with the presence of osteoid supporting the diagnosis of osteosarcoma.…”

Section: Introductionmentioning

confidence: 84%

“…Histopathology of fine‐needle biopsies is a minimally invasive technique that allows for a quick and adequate sample collection with no disruption of the skin or other tissues surrounding the mass. Gel, cell‐block, and needle rinse cell block preparations have been used on cytologic specimens to concentrate nucleated cells for diagnostic viewing 1–4 . To the authors' knowledge, this is the first time a FNA biopsy expelled directly in formalin has been used to confirm a cytologic suspicion of osteosarcoma or any tumor from cytology to histopathology without using cell‐block, gel, or other special substrates or techniques.…”

Section: Discussionmentioning

confidence: 99%

“…Since osteosarcoma is a solid tissue tumor that is fairly exfoliative compared to other mesenchymal tumors, the concentration of cells was not a necessity. Cell block use on effusions has recently been published on mesothelial and carcinomatous effusions, which replaces resident effusion fluid (supernatant) with phosphate buffered formalin and the cells are subject to vortex, which has the potential to disrupt any cohesiveness or tissue architecture that the tissue cells may have 4 …”

Section: Introductionmentioning

confidence: 99%

“…Cell block use on effusions has recently been published on mesothelial and carcinomatous effusions, which replaces resident effusion fluid (supernatant) with phosphate buffered formalin and the cells are subject to vortex, which has the potential to disrupt any cohesiveness or tissue architecture that the tissue cells may have. 4 The CytoHisto method differs from the needle rinse cell block technique again because saline is not used, and it does not require the concentration of the aspirated cellular material from the lesion. With all of these techniques, CytoHisto, cell-block, needle rinse cell block, and routine histopathology, the final goal and outcome are to maintain tissue architecture, preserve diagnostic material, and perform additional stains (whether routine, IHC, or special) to arrive at a definitive diagnosis.…”

mentioning

confidence: 99%

See 4 more Smart Citations

Osteosarcoma diagnosed in a dog using a formalin‐fixed fine‐needle aspirate biopsy

Etzioni

2022

Veterinary Clinical Pathol

View full text Add to dashboard Cite

Background Fine‐needle aspirate (FNA) biopsy is considered a quick technique to access and identify the cell types present in a pathologic lesion or make a diagnosis. Often, clinicians want to know if they are dealing with an inflammatory lesion with or without infectious agents or a neoplastic lesion. At times, neoplastic lesions may be confounded by the presence of inflammatory cells. Objectives We aimed to evaluate the application of a formalin‐fixed FNA, designated the cytologic/histopathologic (CytoHisto) technique, to determine the diagnostic quality and ability to arrive at a definitive diagnosis without the use of concentrated cell block or invasive full tissue biopsy procedures during sample collection. Methods A 10‐cc syringe with a 22‐gauge 1‐inch needle attached was used to obtain a sizable FNA biopsy sample from a thigh mass in a dog. The needle was removed from the syringe, and the material was expulsed from the syringe directly into 10% buffered formalin. After 24–48‐h fixation, a strainer was used to facilitate placement of the fixed granular material into a micromesh biopsy processing/embedding cassette, and the sample was processed as a routine histopathology sample. A microtome was used to make thin sections stained with H&E initially. Then, subsequent sections were stained with immunohistochemical (IHC) stains vimentin, MUM1, and CD18. Alkaline phosphatase staining was performed on a previously Wright's‐stained cytology following IHC results. All sections were coverslipped and viewed under a light microscope. Results When unable to perform incisional or excisional biopsies, this CytoHisto, FNA in formalin technique was useful for collection and subsequent processing as a histopathology sample, with sectioning and then staining with H&E and IHC stains. Neoplastic cells were strongly immunoreactive for vimentin but negative for MUM1 and CD18. Scattered leukocytes within the background stained positively with CD18. Conclusions The CytoHisto technique is minimally invasive and allows for sectioning similar to a full‐thickness excisional or incisional biopsy with subsequent H&E and IHC staining, and special stains allow for a definitive diagnosis of osteosarcoma. The CytoHisto technique is a practical diagnostic technique to pursue in clinical practice that minimizes patient invasiveness and maximizes sample collection time, similar to the routine FNA technique.

show abstract

mentioning

confidence: 61%

Section: Introductionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Osteosarcoma diagnosed in a dog using a formalin‐fixed fine‐needle aspirate biopsy

Etzioni

2022

Veterinary Clinical Pathol

View full text Add to dashboard Cite

show abstract

Localized pleural mesothelioma in a dog

Inanaga,

Yoneji,

Ozaki

2023

Journal of Comparative Pathology

View full text Add to dashboard Cite

Retrospective evaluation of toceranib phosphate (Palladia) in the treatment of canine carcinomatosis and mesothelioma

Hicks,

Leeper,

Curran

2024

Vet Comparative Oncology

View full text Add to dashboard Cite

Canine carcinomatosis (CC) and mesothelioma (CM) are rare but aggressive neoplasms that historically have been associated with poor prognoses. There is limited information regarding treatment for CC and CM. The purpose of this retrospective study was to evaluate the efficacy and tolerability of toceranib phosphate (Palladia) in dogs with CC and CM. Cases were solicited from the American College of Veterinary Internal Medicine (ACVIM) Oncology listserv and retrospectively reviewed. For eligibility, a cytologic and/or histopathologic diagnosis of CC or CM was required. A total of 23 cases were included (CC = 14, CM = 8, both = 1). Eighty‐two percent (19/23) of dogs presented with effusion. The best overall response rate (BORR) was 30.4% (13% complete response [CR], 17.3% partial response [PR]). Stable disease (SD) was appreciated in 14 dogs (60.8%) including the four dogs without effusion. The most common toceranib‐related adverse events were either Grade 1 and 2 diarrhea or hyporexia. The median progression‐free survival (PFS) was 171 days (range, 7–519 days) and overall median survival time (MST) was 301 days (range, 49–875 days) for all dogs. When evaluating dogs solely with effusion, the median PFS and overall MST were 171 days (range, 7–519 days) and 285 days (range, 49–875 days), respectively. This report demonstrates that toceranib is both well tolerated and a potential treatment for CC and CM. A randomised, controlled, prospective study would be needed to objectively assess the survival benefit of toceranib in the management of CC and CM, with and without effusion.

show abstract

Comparison of effusion cell block and biopsy immunohistochemistry in mesothelial hyperplasia, mesothelioma, and carcinoma in dogs

Cited by 7 publications

References 38 publications

Osteosarcoma diagnosed in a dog using a formalin‐fixed fine‐needle aspirate biopsy

Osteosarcoma diagnosed in a dog using a formalin‐fixed fine‐needle aspirate biopsy

Localized pleural mesothelioma in a dog

Retrospective evaluation of toceranib phosphate (Palladia) in the treatment of canine carcinomatosis and mesothelioma

Contact Info

Product

Resources

About