Comparison of Different Extraction Solvents for Characterization of Antioxidant Potential and Polyphenolic Composition in Boletus edulis and Cantharellus cibarius Mushrooms from Romania

Abstract

Edible mushrooms are well-known for their nutritional benefits and low energy density. In addition, mushroom extracts contain various bioactive compounds that account for their antioxidant activity; the applied extraction conditions influence the extraction efficiency of such compounds. Therefore, this study investigates the effects of four extractants on the content of polyphenols and antioxidant properties of Boletus edulis and Cantharellus cibarius mushrooms, aiming to optimize the extraction process. Powders of B. edulis and C. cibarius mushrooms were subjected to extraction with acidic water (10% CH3COOH), ethanol/water/acetic acid (15:76.5:8.5, v/v/v), hexane, and diethyl ether to measure their total phenolic content (TPC), total flavonoid content (TFC), and Trolox equivalent antioxidant capacity (TEAC). Furthermore, the level of individual polyphenolic compounds in these extracts was quantified using an HPLC-DAD-ESI-MS method. Results showed that the type of solvent significantly influenced the TPC and TEAC of mushroom powder but insignificantly influenced the TFC. A very strong positive correlation was found between TPC and TEAC, but no correlation was found between TFC and TEAC or TPC and TFC. Acidic water extracted the highest amount of polyphenolic compounds from these mushroom powders. Therefore, the aqueous extract showed the highest TPC and strongest antioxidant activity. Thus, acidic water is recommended for polyphenol analysis in B. edulis and C. cibarius mushrooms.

Influence of Convective and Vacuum-Type Drying on Quality, Microstructural, Antioxidant and Thermal Properties of Pretreated Boletus edulis Mushrooms

“…The TPC of control sample was significantly higher than that of the blanched sample (459.391 ± 12.421 mg GAE/100 g DW). Our findings regarding the TPC of fresh wild B. edulis mushrooms are similar to other reported ones based on comparable analysis approach, e.g., 1277.5 mg GAE/100 g DW [25], 1250 mg GAE/100 g DW [26], despite that lower [27] or greater contents have also been reported [28,29].…”

Implementation of an Analytical Method for Spectrophotometric Evaluation of Total Phenolic Content in Essential Oils

“…When the r-value is in-between 0.00-0.19, the correlation is very weak; weak between 0.20 and 0.39, moderate between 0.40 and 0.59, strong between 0.60 and 0.79, and very strong between 0.80 and 1.0. The significance of the correlation is interpreted based on the p-value as follows: p ≥ 0.05 NS , not significant trend; 0.01 ≤ p < 0.05, weakly significant; 0.005 ≤ p < 0.01, mildly significant; 0.001 ≤ p < 0.005, moderately significant; p < 0.001, strongly significant [30]. Considering the values resulting for r (0.999) and p (2.64 × 10 −12 ), a very strong positive correlation was found between the two variables of the calibration curve, demonstrating its linearity within the range of the tested standards.…”

“…It was performed using the method published by Fogarasi et al (2021) [30]. Separation, identification, and quantification of individual phenolic compounds were performed on a liquid chromatography system (1200 HPLC; Agilent Technologies Inc., Palo Alto, CA, USA).…”

Apricot Kernel: Bioactivity, Characterization, Applications, and Health Attributes

“…Apricot kernel has been known to possess a wide variety of biologically active components. Generally, microwave extraction, supercritical pressurized liquid extraction, ultrasound-assisted extraction, and microwave solvent extraction methods have been employed for the extraction of the different classes of bioactive compounds [ 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 ]. Different techniques adapted to extract the bioactive compounds from the apricot kernel are represented in Table 2 .…”