Comparison of CZE, MEKC, MEEKC and non-aqueous capillary electrophoresis for the determination of impurities in bromazepam

Hansen, Steen Honoré; Sheribah, Z. A.

doi:10.1016/j.jpba.2005.03.017

Cited by 40 publications

(26 citation statements)

References 17 publications

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“…A Chinese research group reported the development of CE methods for the simultaneous determination of some hydrazine related impurities (Liu et al, 1996). Hansen and Sheribah (2005) evaluated a series of electrically driven separation techniques: CZE, MEKC, and microemulsion electrokinetic chromatography (MEEKC) for the determination of residual alkylating impurities in bromazepam API. However, the poor sensitivity of the techniques posed a problem even when specialized detection cells (e.g.…”

Section: Tlc/hptlc Methodsmentioning

confidence: 99%

Genotoxic Impurities in Pharmaceuticals

Jouyban¹,

Parsa²

2012

Toxicity and Drug Testing

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Section: Tlc/hptlc Methodsmentioning

confidence: 99%

Genotoxic Impurities in Pharmaceuticals

Jouyban¹,

Parsa²

2012

Toxicity and Drug Testing

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“…Recent comparisons have shown MEEKC separations to be superior to MEKC for a number of applications. Hansen and Sheribah [13] compared MEEKC to MEKC for the separation of bromazepam and five of its impurities, the order of separation obtained was very similar for both methods, however MEEKC provided much faster separations. Gong et al [14] used MEEKC to separate a mixture of six biphenyl nitrile compounds and three impurities, obtaining superior results to those achieved with MEKC.…”

Section: Comparison Of Meekc With Mekc and Other Modes Of Cementioning

confidence: 98%

“…The separation of bromazepam and its impurities was performed using CZE, MEKC, MEEKC and NACE [13]. Shorter migration times were achieved with MEEKC but only NACE achieved full resolution.…”

Section: Comparison Of Meekc With Mekc and Other Modes Of Cementioning

confidence: 99%

“…1 = thiamine nitrate, 2 = anhydrous caffeine, 3 = acetaminophen, 4 = riboflavin, 5 = guaifenesen, 6 = pseudoephedrine hydrochloride, 7 = ascorbic acid, 8 = ethenzamine, 9 = methylephedrine hydrochloride, 10 = dihydrocodeine phosphate, 11 = ibuprofen, 12 = noscapine, 13 = carbinoxamine malaete, 14 = bromhexine hydrochloride [88]. Determination of impurities in bromazepan: comparison to CZE and MEKC 2% lithium docecyl sulphate, 6.6% n-butanol, 1% n-octanol in 100 mM formic acid [13] Analysis of corticosteroids: effects of surfactants and CDs on selectivity 4.0% STDC, 2.5% Brij 76, 6.6% n-butanol, 1.36% n-heptane and 85.54% of phosphate buffer pH 2.5 containing 5 mM b-CD [58] W/O MEEKC for pharmaceutical analysis 10% w/w SDS, 2% w/w octane, 78% w/w butanol and 0.07 M sodium acetate pH 8…”

Section: Chiral Separationsmentioning

confidence: 99%

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Recent advances in the development and application of microemulsion EKC

et al. 2007

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Microemulsion EKC (MEEKC) is an electrodriven separation technique. Separations are typically achieved using oil-in-water microemulsions, which are composed of nanometre-sized oil droplets suspended in an aqueous buffer. The droplets are stabilised by a surfactant and a cosurfactant. The novel use of water-in-oil microemulsions has also been investigated. This review summarises the advances in the development of MEEKC separations and also the different areas of application including determination of log P values, pharmaceutical applications, chiral analysis, natural products and bioanalytical separations and the use of new methods such as multiplexed MEEKC and high speed MEEKC. Recent applications (2004-2006) are tabulated for each area with microemulsion composition details.

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“…Different methods such as non-aqueous titration [8] flow injection [9], sequential injection [10], voltammetric [4,11], spectrophotometric [12,13], chromatographic [14 -16], capillary electrophoresis [17], and ion selective sensor methods [18] have been reported for the determination of bromazepam in pharmaceutical preparations, blood and plasma. Though BP recommends non-aqueous titration and spectrophotometric methods for the analysis of bromazepam in raw materials [8], there is no official method for its analysis in tablets.…”

Section: Introductionmentioning

confidence: 99%

High Performance Liquid Chromatography (HPLC) Stability Indicating Method for the Determination of Bromazepam Via its Copper (II) Chelates

Takele

Attaya²,

Hymete³

et al. 2017

PHARMSCI

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Introduction:Bromazepam is hydrolyzed in acidic aqueous solution leading to a series of degradation products. The rate of acidic hydrolysis is believed to be dependent on the state of protonation of the pyridyl and azomethine nitrogen atoms. Stability test is important in pharmaceutical industry to provide evidence on how the quality of an active substance or pharmaceutical product varies with time under the influence of a variety of environmental factors. Objective:The aim of the study was to develop a simple stability indicating method for the determination of bromazepam. Method:Bromazepam solution was prepared and forced degradation of bromazepam was performed under acid hydrolysis using sulphuric acid. High performance liquid chromatography determination of pure and degraded bromazepam and bromazepam-copper (II) complex was performed using reversed phase octyl C-8 column under isocratic conditions and the chromatographic conditions were set as follows; the flow rate of the mobile phase was 1.5 mL/min; injection volume was 10 μL, column temperature was 30 o C and the detector wavelength being 309 nm. Results:Bromazepam, its degradation product and bromazepam chelated with copper (II) were determined using the developed mobile phase with flow rate of 1.5 mL/min. Good separation with sharp peak, minimum tailing and retention time repeatability was obtained. The rate order, rate constant and half-life of degradation were also determined, and it was observed that the degradation reaction follows the first order kinetics. Conclusion:Chromatographic separation of bromazepam chelated with copper (II) was achieved and the method can be further used in drug manufacturing quality control.

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Comparison of CZE, MEKC, MEEKC and non-aqueous capillary electrophoresis for the determination of impurities in bromazepam

Cited by 40 publications

References 17 publications

Genotoxic Impurities in Pharmaceuticals

Genotoxic Impurities in Pharmaceuticals

Recent advances in the development and application of microemulsion EKC

High Performance Liquid Chromatography (HPLC) Stability Indicating Method for the Determination of Bromazepam Via its Copper (II) Chelates

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