Comparison of cytotoxicity of Ag/ZnO and Ag@ZnO nanocomplexes to human umbilical vein endothelial cells <i>in vitro</i>

Yan, Dejian; Zhang, Xue; Li, Shuang; Zhong, Cheng

doi:10.1002/jat.4125

Cited by 6 publications

(8 citation statements)

References 52 publications

(62 reference statements)

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“…Cells were exposed to different concentrations (0, 5, 10, 25, 50, 100, and 200 µg/mL) of pure MgO NPs and Ag (1%, 5%) MgO NPs for 24 h. Results showed that pure and Ag-doped MgO NPs did not induce significant cytotoxicity to HUVE cells. These results suggested that Ag-doped Mg NPs have the potential to be applied for photocatalysis without generating toxicity to human cells [36,62].…”

Section: Cytotoxicity Assaymentioning

confidence: 88%

Facile Synthesis, Characterization, Photocatalytic Activity, and Cytotoxicity of Ag-Doped MgO Nanoparticles

et al. 2021

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Due to unique physicochemical properties, magnesium oxide nanoparticles (MgO NPs) have shown great potential for various applications, including biomedical and environmental remediation. Moreover, the physiochemical properties of MgO NPs can be tailored by metal ion doping that can be utilized in photocatalytic performance and in the biomedical field. There is limited study on the photocatalytic activity and biocompatibility of silver (Ag)-doped MgO NPs. This study was planned for facile synthesis, characterization, and photocatalytic activity of pure and silver (Ag)-doped MgO NPs. In addition, cytotoxicity of pure and Ag-doped MgO NPs was assessed in human normal umbilical vein endothelial cells (HUVECs). Pure MgO NPs and Ag-doped (1, 2, 5, and 7.5 mol%) MgO NPs were prepared via a simple sol-gel procedure. X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared (FTIR), photoluminescence (PL), and X-ray photoelectron spectroscopy (XPS) were used to characterize the prepared samples. XRD results showed the preparation of highly crystalline NPs with no impurity peaks. TEM and SEM studies indicate smooth surfaces with almost spherical morphology of MgO NPs, and Ag-doping did not change the morphology. Elemental composition study suggested that Ag is uniformly distributed in MgO particles. Intensity of the PL spectra of MgO NPs decreased with increasing the concentration of Ag dopants. In comparison to pure MgO NPs, Ag-MgO NPs showed higher degradation of methylene blue (MB) dye under UV irradiation. The improved photocatalytic activity of Ag-MgO NPs was related to the effect of dopant concentration on reducing the recombination between electrons and holes. Cytotoxicity studies showed good biocompatibility of pure and Ag-doped MgO NPs with human normal umbilical vein endothelial cells (HUVECs). These results highlighted the potential of Ag-doped MgO NPs in environmental remediation.

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Section: Cytotoxicity Assaymentioning

confidence: 88%

Facile Synthesis, Characterization, Photocatalytic Activity, and Cytotoxicity of Ag-Doped MgO Nanoparticles

et al. 2021

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“…ZnO NPs decreased the viability of several cell types in vitro, such as HepG2 cells [ 14 ], HUVECs [ 15 ], THP-1 macrophages [ 16 ], peritoneal macrophages [ 17 ], Chinese hamster lung fibroblasts (V79 cells) [ 18 ], keratinocytes (HaCat) and L929 fibroblasts [ 11 ]. In our study, in vitro treatment with ZnO nanocrystals for 24 h resulted in one of the highest CC50 values found for both VERO and RAW 264.7 cells.…”

Section: Discussionmentioning

confidence: 99%

“…Very few studies have evaluated the impact of the association between ZnO NPs and varying concentrations of AgO NPs on cell lines in vitro [ 11 , 15 ]. In comparative studies, for example, it has been reported that Ag NPs were less toxic than ZnO NPs at the same concentrations as HepG2 cells [ 19 ].…”

Section: Discussionmentioning

confidence: 99%

Toxicity Assessment of New Ag-ZnO/AgO Nanocomposites: An In Vitro and In Vivo Approach

do Carmo Neto,

Franco,

Braga

et al. 2024

JFB

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Zinc oxide nanoparticles (ZnO NPs) are metal oxide nanomaterials, which are important for several applications: antibacterial, anthelmintic, antiprotozoal and antitumoral, among others. These applications are mainly related to the ability to spontaneously produce and induce the production of reactive oxygen species that are important components for the destruction of pathogens and tumor cells. While trying to potentiate ZnO NPs, studies have associated these NPs with silver oxide (AgO) or silver (Ag) NPs. It has already been reported that this combination (Ag-ZnO/AgO NPs) is able to enhance the microbicidal potential. Although possessing much potential for several purposes, it is important to evaluate whether this association also poses the risk of toxicity to cells and experimental models. Therefore, this work aimed to evaluate the toxicity of various Ag-ZnO/AgO NP nanocomposites, in vitro and in vivo. Accordingly, ZnO nanocrystals and nanocomposites with various concentrations of AgO (ZnO:5Ag, ZnO:9Ag or ZnO:11Ag) were used in different cytotoxicity models: Galleria mellonella (G. mellonella), cell lines (VERO and RAW 264.7) and C57BL/6 mice. In the G. mellonella model, four concentrations were used in a single dose, with subsequent evaluation of mortality. In the case of cells, serial concentrations starting at 125 µg/mL were used, with subsequent cytotoxicity assessment. Based on the safe doses obtained in G. mellonella and cell models, the best doses were used in mice, with subsequent evaluations of weight, biochemistry as also renal and liver histopathology. It was observed that the toxicity, although low, of the nanocomposites was dependent upon the concentration of AgO used in association with ZnO NPs, both in vitro and in vivo.

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“…There are extensive studies which investigated the toxicity of metal particles to ECs (C. M. Chen et al, 2020; Duan et al, 2014; Gholinejad et al, 2019; Gonzalez‐Palomo et al, 2021; Guo et al, 2018; Guo et al, 2016; Khan et al, 2019; Lee et al, 2019; Liang et al, 2020; Liang et al, 2016; X. Liu et al, 2021; Poier et al, 2020; Shi et al, 2014; D. P. Wang et al, 2020; W. Wang et al, 2018; Yan et al, 2020; Zeng et al, 2018). Numerous studies have reported that silica NPs (Si NPs) can induced apoptosis, autophagy, inflammation and oxidative stress through multiple signaling pathways, which in turn trigger vascular endothelial cell injury (Duan et al, 2014; Guo et al, 2018; Guo et al, 2016; Lee et al, 2019; X. Liu et al, 2021; D. P. Wang et al, 2020; W. Wang et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…And, zinc oxide NPs (ZnO NPs) have been reported to cause damage in ECs (C. M. Chen et al, 2020; Liang et al, 2016; Poier et al, 2020). Curiously, another study showed that Ag‐coated ZnO and Ag‐doped ZnO nanocomplexes were less cytotoxic than ZnO NPs in HUVEC (Yan et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

The effect of nanoparticles of cobalt–chromium on human aortic endothelial cells in vitro

Zhu

Zhang

Liu

et al. 2021

J of Applied Toxicology

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Despite advances in stent technology for vascular interventions, in‐stent restenosis (ISR) remains a main complication. The corrosion of cobalt–chromium (CoCr) alloy coronary stents has been identified to be associated with ISR, whereas its role in ISR has not been elucidated. In the current work, CoCr nanoparticles, simulated corrosion products of CoCr alloy, were used to investigate their effect on the endothelial cells. It has been demonstrated that the cell viability declines and the cell membrane is damaged, indicating the cytotoxicity of CoCr nanoparticles. The expression of GRP78, CHOP, and cleaved‐caspase12 proteins has increased when exposed to CoCr nanoparticles, suggesting that CoCr nanoparticles induced cell apoptosis through endoplasmic reticulum (ER) stress‐mediated apoptotic pathway. An increased release of adhesion and inflammatory mediators was also induced by CoCr nanoparticles, including ICAM‐1, VCAM‐1, IL‐1β, IL‐6, and TNF‐α. Our results demonstrated that CoCr nanoparticles could trigger apoptosis, adhesion, and inflammation. These findings indicated potential damaging effects of CoCr nanoparticles on the vascular endothelium, which suggested corrosion of CoCr alloy may promote the progression and development of ISR.

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Comparison of cytotoxicity of Ag/ZnO and Ag@ZnO nanocomplexes to human umbilical vein endothelial cells in vitro

Cited by 6 publications

References 52 publications

Facile Synthesis, Characterization, Photocatalytic Activity, and Cytotoxicity of Ag-Doped MgO Nanoparticles

Facile Synthesis, Characterization, Photocatalytic Activity, and Cytotoxicity of Ag-Doped MgO Nanoparticles

Toxicity Assessment of New Ag-ZnO/AgO Nanocomposites: An In Vitro and In Vivo Approach

The effect of nanoparticles of cobalt–chromium on human aortic endothelial cells in vitro

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