Comparison of bacteriological culture method and multiplex real-time PCR for detection of mastitis

Prevalence and Molecular Characteristics of Enterococci Isolated from Clinical Bovine Mastitis Cases in Ningxia [Letter]

“…Multiplex rPCR has proven to be extremely reliable in the diagnosis of mastitis since it can detect a huge number of bacteria in a relatively short time when compared to traditional methods, and mixed-infection mastitis pathogens are also more easily identified. 4 , 5 Identification is also possible for clinical pathogen samples that fail to grow in culture. The low concentration of bacteria in milk has been suggested as one of the reasons for the absence of growth in bacterial culture.…”