Comparison of APTIMA Trichomonas vaginalis transcription-mediated amplification to wet mount microscopy, culture, and polymerase chain reaction for diagnosis of trichomoniasis in men and women

Inducing Immune Protection Against Trichomonas vaginalis: A Novel Vaccine Approach to Prevent HIV Transmission

“…These tests may be conducted years after sample collection depending on proper storage conditions unlike wet mount which results in much less sensitivity unless maintained under strict time and temperature conditions (Van Der Pol et al, 2006). Despite high sensitivity for both male and female samples (Seña et al, 2007), greater than wet mount and culture (Crucitti et al, 2003;Lusk et al, 2010;Roth et al, 2011), in the range of 70-98% and specificity from 94-100% (Huppert et al, 2007;Nye et al, 2009;Seña et al, 2007;Wendel et al, 2002) this method is problematic as it is not point of care and remains unconfirmed for use in clinical settings.…”

“…Moreover, incorrect temperature or too much time elapsing between sampling and assessment results in loss of movement creating difficulty of differentiation from lymphocytes or nuclei of vaginal epithelial cells present in the sample (Garber, 2005). Sensitivities range from 52-61.5% and specificity near 100% (Huppert et al, 2007;Nye et al, 2009;Van Der Pol et al, 2006;Wendel et al, 2002). …”

“…Additionally, this method is expensive and time consuming (Domeika et al, 2010) despite the availability of commercial culture systems (InPouch TV system: BioMed Diagnostics). Studies have found sensitivities ranging between 75-83% and specificity near 100% (Huppert et al, 2007;Nye et al, 2009;Wendel et al, 2002). Wet mount is an attractive diagnostic tool due to its simplicity and cost effectiveness, and is consequently the most widely used (Huppert et al, 2007).…”

Investigation of the presence of Trichomonas vaginalis in infertile Turkish women

“…Although this method is inexpensive and fast, sensitivity is not high and the parasite is detected in only 60% of culture positive samples. Novel diagnostic methods such as nucleic acid amplification are more sensitive than routine diagnostic methods, but more expensive and not generally available [7,8].…”

Trichomonas Vaginalis in Hiv-Infected women: A Risk Factor for High Risk Human Papillomavirus

“…The true prevalence of Trichomonas is likely underestimated due to a lack of reporting, low rates of screening in asymptomatic patients, and the poor sensitivity of microscopy for diagnosis [3,4]. Trichomonas infection affects 3.2% of American women, but non-Hispanic, black women have disproportionately high rates of Trichomonas compared to non-Hispanic, white women (13% vs.1%) [5,6].…”