Comparison of adventitious shoot formation from node and leaf explants of various carnation (Dianthus caryophyllusL.) cultivars

Nontaswatsri, Chalermsri; Fukai, Seiichi; Touma, T.; Goi, Masanori

doi:10.1080/14620316.2002.11511532

Cited by 10 publications

(7 citation statements)

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“…Therefore, growth regulators are noticeably important for in vitro shoot proliferation, but some internal factors and nutrient conditions can modify their activities (Park et al, 2001). Shoot regeneration also depends upon endogenous levels of growth regulators in the explant and the position of node, as different types of buds showed different regeneration efficiencies in grape (Nontaswatsri et al, 2002). The present study indicates that BAP in combination with GA 3 effectively elongated the shoots (Fig.…”

Section: Growth Of In Vitro Shoot Culturesmentioning

confidence: 50%

Optimizing the concentrations of plant growth regulators for in vitro shoot cultures, callus induction and shoot regeneration from calluses of grapes

Khan¹,

Ahmed

Hafiz

et al. 2015

OENO One

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Aim: To optimize the concentrations of growth regulators in the media for the proficient micropropagation of grapevine (Vitis vinifera L.) cv. King’s Ruby.Methods and results: Apical meristems of the grape cultivar were used to establish in vitro shoot cultures. Nodal explants, each containing an axillary bud, taken from in vitro grown shoots were inoculated in shoot proliferation medium, i.e., half strength Murashige and Skoog (MS) medium supplemented with benzyl aminopurine (BAP), kinetin, glycine and gibberellic acid (GA3). A higher number of shoots (5.33) with greater shoot length (2.75 cm) was produced in the medium supplemented with 1.0 mg L-1 BAP and 0.1 mg L-1 GA3. Calluses were induced from leaf explants taken from in vitro grown shoots. Callus induction was greater (73.00%) on the medium containing 2.0 mg L-1 2,4-dichlorophenoxyacetic acid (2,4-D), 0.3 mg L-1 BAP and 0.2 mg L-1 α-naphthaleneacetic acid (NAA). The maximum frequency of shoot regeneration (53.33%) was achieved on the medium supplemented with 1.5 mg L-1 BAP and 0.5 mg L-1 NAA, and the regenerated shoots successfully formed roots on growth regulator-free half strength MS medium.Conclusion: Optimizing the concentration of BAP and GA3 and omitting the glycine and kinetin in the culture medium increased the number and length of shoots. Similarly, for inducing the callus of the leaf explants, taken from in vitro grown shoots, it is recommended to adjust the medium with the higher concentration of 2,4-D and lower concentrations of BAP. Moreover, the maximum number of shoots was regenerated on a medium supplemented with relatively high levels of both BAP and NAA (1.5 and 0.5 mg L-1, respectively). Finally, we suggest the half strength MS medium that is free from growth regulators for the root formation of the regenerated shoots.Significance and impact of the study: Optimizing the concentration of growth regulators is crucial for the efficient micropropagation of a grape cultivar. Knowing the specific balance between the growth regulators is necessary to establish in vitro shoot cultures, callus induction and shoot regeneration and, hence, to propagate disease-free true to type grape cultivars in a short time.

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Section: Growth Of In Vitro Shoot Culturesmentioning

confidence: 50%

Optimizing the concentrations of plant growth regulators for in vitro shoot cultures, callus induction and shoot regeneration from calluses of grapes

Khan¹,

Ahmed

Hafiz

et al. 2015

OENO One

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“…The induction of secondary somatic embryos on medium containing 1 mg/l BA was suppressed and primary embryos proliferated into shoots. Stimulation of adventitious shoot production from stem (Nugent et al 1991), petal (Nakano et al 1994) and leaf explants (Nontvaswatsri et al 2002) has also been reported in carnation when BA is used.…”

Section: Secondary Somatic Embryogenesismentioning

confidence: 94%

Secondary somatic embryogenesis of carnation (Dianthus caryophyllus L.)

Karami

Deljou

Kordestani

2007

Plant Cell Tiss Organ Cult

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This is the first report describing culture conditions necessary to induce secondary embryogenesis in two carnation cultivars, Nelson and Spirit. In the first step, embryogenic calli were induced on petal explants followed by development of primary somatic embryos from the calli. In the second stage, secondary somatic embryos were obtained when precotyledonary and cotyledonary primary embryos were isolated and transferred onto a series of culture media all containing MS basal salt mixture, and supplemented with different concentrations of 2,4-D, BA, sucrose and mannitol. The highest rate of secondary embryogenesis occurred on mannitol containing media. Secondary somatic embryos were converted into plantlets when they were transferred onto growth regulator-free half-strength MS medium and successfully acclimated in the greenhouse.

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“…Genotypic differences in shoot organogenesis have been observed in a wide range of species. Some genotypes exhibit high regenerative capacity, while others are either recalcitrant or exhibit no capacity at all (Kuehnle and Sugii 1991;Nontaswatsri et al 2002). Explant types may also influence capacity for indirect shoot organogenesis, which may be related to the totipotency of cells at their developmental stages (Agarwal and Ranu 2000;Bacchetta et al 2003).…”

Section: Introductionmentioning

confidence: 96%

Effects of genotype, explant source, and plant growth regulators on indirect shoot organogenesis in Dieffenbachia cultivars

Shen

Kane

Chen

2008

In Vitro Cell.Dev.Biol.-Plant

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The capacity for indirect shoot organogenesis of leaf and root explants of four Dieffenbachia cultivars were examined on a modified Murashige and Skoog (MS; Physiol Plant 15:473-495, 1962) medium supplemented with different plant growth regulators in 112 combinations. Callus formation was only observed from leaf explants on MS supplemented with 1-10 μM thidiazuron (TDZ) and 0.5-1.0 μM 2,4-dichlorophenoxyacetic acid (2,4-D) regardless of cultivars. The combination of 5 μM TDZ and 1 μM 2,4-D resulted in the greatest callus formation frequency among the four cultivars tested. Significant differences in callus and shoot formation from leaf explants were also observed among cultivars. Cultivars Camouflage, Camille, Octopus, and Star Bright produced green nodular, brown nodular, yellow friable, and green compact calli with corresponding maximum callus formation frequencies of 96%, 62%, 54%, and 52%, respectively. A maximum of 6.7 shoots/callus was observed in cv. Camouflage, followed by cvs. Camille and Star Bright at 3.7 and 3.5, respectively. Calli of cv. Octopus displayed no capacity for shoot organogenesis. Regardless of cultivar, callus formation was not observed on root explants. Regenerated shoots were successfully acclimatized in a shaded greenhouse condition with 100% survival.

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Comparison of adventitious shoot formation from node and leaf explants of various carnation (Dianthus caryophyllusL.) cultivars

Cited by 10 publications

References 0 publications

Optimizing the concentrations of plant growth regulators for in vitro shoot cultures, callus induction and shoot regeneration from calluses of grapes

Optimizing the concentrations of plant growth regulators for in vitro shoot cultures, callus induction and shoot regeneration from calluses of grapes

Secondary somatic embryogenesis of carnation (Dianthus caryophyllus L.)

Effects of genotype, explant source, and plant growth regulators on indirect shoot organogenesis in Dieffenbachia cultivars

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