J. Clin. Lab. Anal. volume 29, issue 2, P94-99 2014 DOI: 10.1002/jcla.21734 View full text
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Mayuree Kengkate, Punnee Butthep, Pawinee Kupatawintu, Atthapol Srisuddee, Wasun Chantratita, Oytip Nathalang

Abstract: Background We aimed to compare HPA‐1 to HPA‐6 and HPA‐15 genotyping results obtained by a simple‐probe real‐time polymerase chain reaction (PCR) technique with the multiplex PCR technique. Methods Five hundred DNA samples from the Thai National Stem Cell Donor Registry (TSCDR) of the National Blood Centre, Thai Red Cross Society were included. Human platelet antigen (HPA) genotyping was performed by simple‐probe real‐time PCR and multiplex PCR techniques. Results HPA‐1, HPA‐2, HPA‐3, and HPA‐4 genotyping resu…

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