Comparison between different dilution rates on canine semen freezing using Tris-buffer with the addition of egg-yolk and glycerol

Abstract

ABSTRACTStandardized sperm concentration and volume:volume extension were compared as dilution rates for canine semen freezing. Six proven stud dogs were submitted to two seminal collections by manual stimulation. Semen was evaluated and extended in tris plus egg-yolk and glycerol according to two different dilution rates. The first one was based on a standardized sperm concentration of 200x10 6 spermatozoa/ml and the second was a volume:volume extension at a proportion of one part semen to one part extende…