2009
DOI: 10.4322/rbpv.01803007
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Abstract: An available enzyme-linked immunosorbent assay (ELISA) was studied for the detection of anti-B. canis antibodies in the sera of dogs using, indirect fluorescent antibody test (IFAT) as a reference test. ELISA uses a soluble antigenic preparation of B. canis and the optimal dilutions of the antigen, serum and conjugate were determined by check board titration, using positive and negative reference serum. The soluble antigen preparation of B. canis merozoites was 10 µg.mL , with reference sera from positive and … Show more

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Cited by 28 publications
(18 citation statements)
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References 14 publications
(18 reference statements)
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“…Babesia spp. antigen was prepared by intravenous inoculation of B. vogeli (Jaboticabal strain) into a splenectomized three month-old dog negative for hemoparasites by PCR and serology (FURUTA et al, 2009). Giemsa-stained blood smears were examined microscopically twice daily day for the presence of parasites.…”
Section: Methodsmentioning
confidence: 99%
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“…Babesia spp. antigen was prepared by intravenous inoculation of B. vogeli (Jaboticabal strain) into a splenectomized three month-old dog negative for hemoparasites by PCR and serology (FURUTA et al, 2009). Giemsa-stained blood smears were examined microscopically twice daily day for the presence of parasites.…”
Section: Methodsmentioning
confidence: 99%
“…promastigotes were tested by IFAT (Indirect Fluorescent Antibody Test), as described previously (FURUTA et al, 2009;NAKAGHI et al, 2008;OLIVEIRA et al, 2008). Antigen slides were removed from storage (−20 °C) and allowed to thaw at room temperature for 30 min.…”
Section: Methodsmentioning
confidence: 99%
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“…Immunofluorescent Antibody Test (IFAT) for Ehrlichia canis and Babesia canis -IFAT was performed as described by Camargo (1974) and standardized by Nakaghi et al (2008) for the E. canis system, and as described by Furuta et al (2009), for the B. canis system DNA extraction and nested PCR (nPCR) analysis -Using the commercially available QIAamp ® DNA Blood Mini Kit (cat. No.…”
Section: Methodsmentioning
confidence: 99%
“…The disease usually causes fever, lethargy, anorexia, and jaundice, and clinicopathological abnormalities typically found include regenerative immune-mediated hemolytic anemia, nonregenerative anemia, leukocytosis, leucopenia, and thrombocytopenia (6) . The seroprevalence of B. vogeli, a cosmopolitan species, ranges from 18.8% to 73.3% (7)(8)(9)(10) .…”
Section: Introductionmentioning
confidence: 99%