Comparision of biological and genomic characteristics of five virulent bacteriophages against Enterobacter hormaechei

Chen, Cao-wei; Yuan, Lei; Zhang, Yuan‐song; Mgomi, Fedrick C.; Wang, Yan; Jiao, Xinan

doi:10.1016/j.micpath.2021.105375

Cited by 8 publications

(2 citation statements)

References 38 publications

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“…7). The phage (MN688132.1) targeting Klebsiella pneumoniae (1288317-1338719) is reported by Chen et al, 2022, to be capable of targeting Enterobacter hormaechei as well [51]. E. hormaechei is responsible for nosocomial infections, urinary tract diseases and bacteraemia [53].…”

Section: Discussionmentioning

confidence: 99%

Development of computationally-guided workflow for designing therapeutic phage cocktail: targeting multidrug-resistant (MDR) bacteria

Nawaz

Husnain

Ali

et al. 2023

Preprint

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Background: Antibiotic misuse and overuse have contributed to the emergence of multi-drug resistant bacteria (MDR), a serious public health problem across the globe. Phage cocktails, which combine several phages to destroy various bacterial strains, offer a more thorough and efficient method of battling MDR illnesses. This might revolutionize the looming threat of reemergence of untreatable bacterial diseases. To provide a focused strategy to tackle the rising incidence of MDR bacterial infections, a phage cocktail against Acinetobacter baumannii, Klebsiella pneumoniae, and Pseudomonas aeruginosa was intended to be made computationally. Predicting a group of prophages which can successfully lyse and disrupt these three MDR bacterial strains and might help to lessen the severity and occurrence of illnesses caused by these notorious pathogens. Methods: The genomes of selected MDR bacteria were accessed through NCBI GenBank, and prophages targeting them were selected. The prophages were further annotated for ORFs, putative promotors, virulence factors, transcriptional terminators, and tRNAs. Dot plot was created to investigate the similar phages and phylogenetic analysis was performed. Results: A total of 11 prophages were predicted from three MDR bacterial genomes, the investigation identified 472 ORFs and CDS, rRNA, and tRNA regions in 11 prophage genomes were predicted. The presence of 3 tRNAs encoded by the predicted prophages suggests a possible reliance on the host translation machinery for protein synthesis. The presence of transcription terminators and promotors were detected to understand the transcriptional and translational regulation of prophage genes. The comparative genomic and phylogenetic analyses of predicted prophages provided important insights into diversity and relatedness of the phages. The final selected five prophages included Acinetobacter baumannii prophage (2759376-2809756), Acinetobacter baumannii prophage (3311844-3364667), Klebsiella pneumoniae (1288317-1338719), Klebsiella pneumoniae prophage (1778306-1808606), and Klebsiella pneumoniae prophage (2280703-2325555). Conclusion: In conclusion, our work provides an example of developing a phage cocktail to combat multidrug resistant Acinetobacter baumannii and Klebsiella pneumoniae. Sequence similarity analyses revealed that the cocktail is capable of targeting Enterobacter hormaechei and other carbapenemase-producing K. pneumoniae strains also. The phage cocktail indicates the possibility of being employed as a therapeutic agent for reducing harmful bacterial infections, where conventional antibiotic therapeutics fail.

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Section: Discussionmentioning

confidence: 99%

Development of computationally-guided workflow for designing therapeutic phage cocktail: targeting multidrug-resistant (MDR) bacteria

Nawaz

Husnain

Ali

et al. 2023

Preprint

View full text Add to dashboard Cite

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“…Very similar tolerance to a wide pH range was also shown by the phages KKP 3262 and KKP 3263 described by Wójcicki et al (2021) [ 94 ]. Interestingly, two of five E. hormaechei -specific phages isolated by Chen and coworkers (2022) showed an almost complete resistance to a more alkaline environment (such as pH = 12) [ 95 ].…”

Section: Discussionmentioning

confidence: 99%

Two Newly Isolated Enterobacter-Specific Bacteriophages: Biological Properties and Stability Studies

Cieślik

Harhala

Orwat

et al. 2022

Viruses

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In an era of antibiotic therapy crisis caused by spreading antimicrobial resistance, and when recurrent urinary tract infections constitute a serious social and medical problem, the isolation and complex characterization of phages with a potential therapeutic application represents a promising solution. It is an inevitable, and even a necessary direction in the development of current phage research. In this paper, we present two newly isolated myoviruses that show lytic activity against multidrug-resistant clinical isolates of Enterobacter spp. (E. cloacae, E. hormaechei, and E. kobei), the genomes of which belong to a poorly represented phage group. Both phages were classified as part of the Tevenvirinae subfamily (Entb_43 was recognized as Karamvirus and Entb_45 as Kanagawavirus). Phage lytic spectra ranging from 40 to 60% were obtained. The most effective phage-to-bacteria ratios (MOI = 0.01 and MOI = 0.001) for both the phage amplification and their lytic activity against planktonic bacteria were also estimated. Complete adsorption to host cells were obtained after about 20 min for Entb_43 and 10 min for Entb_45. The phage lysates retained their initial titers even during six months of storage at both −70 °C and 4 °C, whereas storage at 37 °C caused a complete loss in their activity. We showed that phages retained their activity after incubation with solutions of silver and copper nanoparticles, which may indicate possible synergistic antibacterial activity. Moreover, a significant reduction in phage titers was observed after incubation with a disinfectant containing octenidinum dihydrochloridum and phenoxyethanol, as well as with 70% ethanol. The observed maintenance of phage activity during incubation in a urine sample, along with other described properties, may suggest a therapeutic potential of phages at the infection site after intravesical administration.

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Isolation, characterization, and application of lytic bacteriophages for controlling Enterobacter cloacae complex (ECC) in pasteurized milk and yogurt

et al. 2023

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Reducing bacterial pathogen contamination not only improves overall global public health but also diminishes food waste and loss. The use of lytic bacteriophages (phages) that infect and kill bacteria could be a beneficial tool for suppressing bacterial growth during dairy products storage time. Four Enterobacter cloacae (E. cloacae) complex isolates which were previously isolated from contaminated dairy products were used to identify lytic phages in wastewater. Phages specific to multi-drug resistant (MDR) E. cloacae complex 6AS1 were isolated from local sewage. Two novel phages vB_EclM-EP1 and vB_EclM-EP2 were identified as myoviral particles and have double-stranded DNA genome. Their host range and lytic capabilities were detected using spot test and efficiency of plating (EOP) against several bacterial isolates. The phages had a latent period of 30 min, and a large burst size of about 100 and 142 PFU/cell for vB_EclM-EP1 and vB_EclM-EP2, respectively. Both phages were viable at pH ranging 5–9 and stable at 70 °C for 60 min. The individual phages and their cocktail preparations (vB_EclM-EP1 and vB_EclM-EP2) reduced and inhibited the growth of E. cloacae complex 6AS1 during challenge test in milk and yogurt samples. These results indicate that the E. cloacae complex-specific phages (vB_EclM-EP1 and vB_EclM-EP2) have a potential application as microbicidal agents in packaged milk and milk derivatives during storage time. In addition, our environment is a rich sources of lytic phages which have potential use in eliminating multidrug-resistant isolates in food industry as well as in biocontrol.

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Comparision of biological and genomic characteristics of five virulent bacteriophages against Enterobacter hormaechei

Cited by 8 publications

References 38 publications

Development of computationally-guided workflow for designing therapeutic phage cocktail: targeting multidrug-resistant (MDR) bacteria

Development of computationally-guided workflow for designing therapeutic phage cocktail: targeting multidrug-resistant (MDR) bacteria

Two Newly Isolated Enterobacter-Specific Bacteriophages: Biological Properties and Stability Studies

Isolation, characterization, and application of lytic bacteriophages for controlling Enterobacter cloacae complex (ECC) in pasteurized milk and yogurt

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