2016
DOI: 10.21608/besps.2016.8643
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Abstract: Invasive procedures including chorionic villus sampling and amniocentesis in sexlinked diseases increase the risk of fetal loss. Therefore, Noninvasive fetal gender determination using cell-free fetal DNA (cffDNA) in maternal plasma may be promising. Fifty pregnant females with gestational age ranging from six to ten weeks were included. cffDNA were extracted from maternal plasma and amplified by real time and conventional PCR for identification of SRY, DYS14 and DAZ genes as specific genetic markers for male-… Show more

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Cited by 2 publications
(3 citation statements)
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“…In this study, two embryos gave false negative results using SRY sequence, after confirmation by sequencing and comparing this result with that obtained by DYS14 and DAZ sequences, which gave positive results for these two embryos. This agrees with our previous study [27] on cffDNA that reported that positive DYS14 and DAZ gene results correctly diagnosed a male fetus with 100% sensitivity and specificity in spite of the fact that about 60% of cffDNA is fragmented [29]. As previously stated, these false negative results using SRY sequence is due to the fact that it is a single copy gene, while DYS14 and DAZ are multicopy genes [22].…”
Section: Control Of Contaminationsupporting
confidence: 92%
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“…In this study, two embryos gave false negative results using SRY sequence, after confirmation by sequencing and comparing this result with that obtained by DYS14 and DAZ sequences, which gave positive results for these two embryos. This agrees with our previous study [27] on cffDNA that reported that positive DYS14 and DAZ gene results correctly diagnosed a male fetus with 100% sensitivity and specificity in spite of the fact that about 60% of cffDNA is fragmented [29]. As previously stated, these false negative results using SRY sequence is due to the fact that it is a single copy gene, while DYS14 and DAZ are multicopy genes [22].…”
Section: Control Of Contaminationsupporting
confidence: 92%
“…Therefore, female embryos could be falsely classified as males by the DYS14 gene. However, in our previous study on cffDNA [27], using real time PCR, when DYS14 gave positive result and at the same time, SRY and DAZ gave negative results, the case was considered male, however in that study, a cut off value was applied which increased the specificity of the DYS14 to 100%, therefore, the case was considered male.…”
Section: Control Of Contaminationmentioning
confidence: 89%
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