“…Therefore, female embryos could be falsely classified as males by the DYS14 gene. However, in our previous study on cffDNA [27], using real time PCR, when DYS14 gave positive result and at the same time, SRY and DAZ gave negative results, the case was considered male, however in that study, a cut off value was applied which increased the specificity of the DYS14 to 100%, therefore, the case was considered male.…”