Comparative transcriptome and proteome provide new insights into the regulatory mechanisms of the postharvest deterioration of Pleurotus tuoliensis fruitbodies during storage

Wu, Mu-Xiu; Zou, Yuan; Yu, Yinghao; Chen, Bai‐Xiong; Zheng, Qianwang; Ye, Zhiwei; Wei, Tao; Ye, Siqiang; Guo, Li‐Qiong; Lin, Jizhen

doi:10.1016/j.foodres.2021.110540

Cited by 31 publications

(23 citation statements)

References 78 publications

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“…Studies have reported that ATP supply has a great influence on the postharvest quality of vegetables and fruits by maintaining membrane integrity and reducing oxidative stress. , Nevertheless, during the postharvest storage, C. comatus obtained energy mainly through breaking down nutrients, and the decomposition of sugar, protein, and lipids tended to aggravate the mushroom autolysis. , Therefore, attention to these key DAPs is of great help in revealing the mechanism of postharvest mushroom autolysis.…”

Section: Results and Discussionmentioning

confidence: 99%

“…The mixture was then filtered, and the cellulose content was determined by the colorimetric anthrone assay. 15 As for the chitin content, 1 g of the mushroom sample was hydrolyzed by 37% HCl (10 mL) at 110 °C for 4 h. The resulting hydrolysate (1 mL) was then mixed with 2% resorcinol (1 mL) and 75% H 2 SO 4 (7.5 mL). Next, the mixture was heated in a boiling water bath for 30 min and the absorbance was measured at 500 nm after cooling to room temperature.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

“…24,25 Nevertheless, during the postharvest storage, C. comatus obtained energy mainly through breaking down nutrients, and the decomposition of sugar, protein, and lipids tended to aggravate the mushroom autolysis. 15,26 Therefore, attention to these key DAPs is of great help in revealing the mechanism of postharvest mushroom autolysis.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

“…The precipitate was digested by 60% sulfuric acid (H 2 SO 4 ) for 30 min. The mixture was then filtered, and the cellulose content was determined by the colorimetric anthrone assay . As for the chitin content, 1 g of the mushroom sample was hydrolyzed by 37% HCl (10 mL) at 110 °C for 4 h. The resulting hydrolysate (1 mL) was then mixed with 2% resorcinol (1 mL) and 75% H 2 SO 4 (7.5 mL).…”

Section: Methodsmentioning

confidence: 99%

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Proteomics Reveals the Mechanism Underlying the Autolysis of Postharvest Coprinus comatus Fruiting Bodies

Yang

Zheng

Zhou

et al. 2022

J. Agric. Food Chem.

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Autolysis occurs widely in edible mushroom fruiting bodies after harvest, but the mechanism is still unclear. In this study, quantitative proteomics and bioinformatics analyses have been applied for revealing the autolysis mechanism of postharvest Coprinus comatus fruiting bodies. The results indicated that the autolysis mechanism of postharvest C. comatus was complicated. Before pileus opening, the carbohydrate metabolism including cell wall hydrolysis and energy biosynthesis, which were probably regulated by the ribosome, was involved in mushroom autolysis, whereas after pileus opening, the autolysis mechanism was related to the accumulated reactive oxygen species (ROS) and activated mitogen-activated protein kinase (MAPK) signaling pathway based on the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses. Furthermore, the changes in cell wall components and hydrolases, along with the production of ROS and the activities of oxidoreductase in C. comatus, were also verified to confirm the proteomic analysis results.

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Section: Results and Discussionmentioning

confidence: 99%

Section: ■ Materials and Methodsmentioning

confidence: 99%

Section: ■ Materials and Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Proteomics Reveals the Mechanism Underlying the Autolysis of Postharvest Coprinus comatus Fruiting Bodies

Yang

Zheng

Zhou

et al. 2022

J. Agric. Food Chem.

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“…Within sucrose and starch cycle, starch synthesis and degradation are simultaneously, during the preharvest period, taking place with net breakdown thereafter ( Schouten et al, 2016 ). In addition, transcription factors (TFs) like bHLH, MYB, ERF, and LBD play important roles in regulating postharvest physiological changes ( Wu et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Multifaceted analyses reveal carbohydrate metabolism mainly affecting the quality of postharvest bamboo shoots

Yang

et al. 2022

Front. Plant Sci.

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Bamboo shoot is one of nutritious vegetables in China. However, the edible quality of fresh bamboo shoots deteriorates easily after harvest. Here, morphological, physiological, transcriptomic and microRNA sequencing analyses were conducted to investigate the postharvest characteristics of moso bamboo (Phyllostachys edulis) shoots. Rapid decreases of soluble sugars, structural polysaccharides and hydrolyzed tannins, and increases of lignin and condensed tannins were observed in the postharvest bamboo shoots. Differentially expressed genes (DEGs) and miRNAs with opposite trends were mainly enriched in structural polysaccharide metabolism, starch and sucrose metabolism and glycolysis pathways, which were consistent with the changes of carbohydrates. A co-expression network of carbohydrate metabolism was constructed, which was verified by qPCR and yeast one-hybrid (Y1H) assay. Furthermore, the function of one hub glycosyltransferase gene was validated in Arabidopsis, which confirmed that it was involved in xylan biosynthesis. These results are of great significance for revealing the carbohydrate metabolism mechanisms of postharvest bamboo shoots and provide a potential candidate gene for molecular breeding related to xylan in the future.

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Physiological and transcriptional responses of carbohydrate and nitrogen metabolism and ion balance in blueberry plants under nitrogen deficiency

Yang

Duan

et al. 2023

Plant Growth Regul

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Comparative transcriptome and proteome provide new insights into the regulatory mechanisms of the postharvest deterioration of Pleurotus tuoliensis fruitbodies during storage

Cited by 31 publications

References 78 publications

Proteomics Reveals the Mechanism Underlying the Autolysis of Postharvest Coprinus comatus Fruiting Bodies

Proteomics Reveals the Mechanism Underlying the Autolysis of Postharvest Coprinus comatus Fruiting Bodies

Multifaceted analyses reveal carbohydrate metabolism mainly affecting the quality of postharvest bamboo shoots

Physiological and transcriptional responses of carbohydrate and nitrogen metabolism and ion balance in blueberry plants under nitrogen deficiency

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