Comparative transcriptome analysis reveals resistant and susceptible genes in tobacco cultivars in response to infection by Phytophthora nicotianae

Abstract

Phytophthora nicotianae is highly pathogenic to Solanaceous crops and is a major problem in tobacco production. The tobacco cultivar Beihart1000-1 (BH) is resistant, whereas the Xiaohuangjin 1025 (XHJ) cultivar is susceptible to infection. Here, BH and XHJ were used as models to identify resistant and susceptible genes using RNA sequencing (RNA-seq). Roots were sampled at 0, 6, 12, 24, and 60 h post infection. In total, 23,753 and 25,187 differentially expressed genes (DEGs) were identified in BH and XHJ, respectively. By mapping upregulated DEGs to the KEGG database, changes of the rich factor of “plant pathogen interaction pathway” were corresponded to the infection process. Of all the DEGs in this pathway, 38 were specifically regulated in BH. These genes included 11 disease-resistance proteins, 3 pathogenesis-related proteins, 4 RLP/RLKs, 2 CNGCs, 7 calcium-dependent protein kinases, 4 calcium-binding proteins, 1 mitogen-activated protein kinase kinase, 1 protein EDS1L, 2 WRKY transcription factors, 1 mannosyltransferase, and 1 calmodulin-like protein. By combining the analysis of reported susceptible (S) gene homologs and DEGs in XHJ, 9 S gene homologs were identified, which included 1 calmodulin-binding transcription activator, 1 cyclic nucleotide-gated ion channel, 1 protein trichome birefringence-like protein, 1 plant UBX domain-containing protein, 1 ADP-ribosylation factor GTPase-activating protein, 2 callose synthases, and 2 cellulose synthase A catalytic subunits. qRT-PCR was used to validate the RNA-seq data. The comprehensive transcriptome dataset described here, including candidate resistant and susceptible genes, will provide a valuable resource for breeding tobacco plants resistant to P. nicotianae infections.

RNA-seq Gene Profiling Reveals Transcriptional Changes in the Late Phase during Compatible Interaction between a Korean Soybean Cultivar (Glycine max cv. Kwangan) and Pseudomonas syringae pv. syringae B728a

“…Transcriptional profiles of several crop plants after pathogen infections clarified particular metabolic and signaling pathways tightly related to compatible and incompatible interaction ( Cregeen et al, 2015 ; Gyetvai et al, 2012 ; Meng et al, 2021 ; Sonah et al, 2016 ; van Esse et al, 2009 ; Wang et al, 2010 ).…”

Comparative transcriptome analysis of compatible and incompatible Brassica napus—Xanthomonas campestris interactions

“…Although considerable efforts have been made to understand genetic mechanisms of black rot resistance in Brassica crops ( Islam et al, 2017 , 2019 ; Song et al, 2020 ; Sun et al, 2021 ), key genes underlying variation in black rot resistance are thus far largely unknown. Insights into the transcriptional dynamics of plant-pathogen interactions can help to potentially pinpoint genes underlying pathogen resistance ( Meng et al, 2021 ). To elucidate the transcriptional response of B. napus to black rot infection, we performed comparative transcriptome sequencing of three B. napus lines with different levels of susceptibility at three time points after Xcc inoculation.…”

The Same against Many: AtCML8, a Ca2+ Sensor Acting as a Positive Regulator of Defense Responses against Several Plant Pathogens

“…The RipAB effector is responsible for the repression of three CMLs, one CDPK, and a Ca 2+ transporter in potato. In 2021, Meng et al [83] reported that among the root genes, specifically, and rapidly upregulated in tobacco-resistant cultivar to Phytophthora nicotianae, four CMLs were identified, whereas the Loc107773369 gene, which corresponds to the closest tobacco ortholog to CML8, was downregulated. CML8 is the closest homolog to the soybean CaM4 and when overexpressed, CaM4 is responsible for enhanced resistance to the oomycete Phytophthora sojae and two necrotrophic fungi, Alternaria tenuissima and Phomopsis longicolla [84].…”