Comparative Transcriptome Analysis of Two Rice Varieties in Response to Rice Stripe Virus and Small Brown Planthoppers during Early Interaction

Abstract

Rice stripe, a virus disease, transmitted by a small brown planthopper (SBPH), has greatly reduced production of japonica rice in East Asia, especially in China. Although we have made great progress in mapping resistance genes, little is known about the mechanism of resistance.By de novo transcriptome assembling, we gained sufficient transcript data to analyze changes in gene expression of early interaction in response to SBPH and RSV infection in rice. Respectively 648 and 937 DEGs were detected from the disease-resistant (Liaonong 979) and the susceptible (Fengjin) varieties, most of which were up-regulated. We found 37 genes related to insect resistance, which mainly included genes for jasmonate-induced protein, TIFY protein, lipoxygenase, as well as trypsin inhibitor genes and transcription factor genes. In the interaction process between RSV and rice, 87 genes were thought to be related to RSV resistance; these primarily included 12 peroxidase biosynthesis genes, 12 LRR receptor-like protein kinase genes, 6 genes coding pathogenesis-related proteins, 4 glycine-rich cell wall structural protein genes, 2 xyloglucan hydrolase genes and a cellulose synthase. The results indicate that the rice-pathogen interaction happened both in disease-resistant and susceptible varieties, and some genes related to JA biosynthesis played key roles in the interaction between SBPHs and rice. When rice was infected by RSV a hypersensitive reaction (HR) in the disease-resistant variety was suppressed, which resulted from an increase in peroxidase expression and down-regulation of LRR receptor-like protein kinase and pathogenesis-related proteins, while, the changes of peroxidase biosynthesis, glycine-rich cell wall structural protein, cellulose synthase and xyloglucan endotransglucosylase/hydrolase could lead to the strengthening of physical barriers of rice, which may be an important resistance mechanism to RSV in rice.

Assessment of reference gene stability in Rice stripe virus and Rice black streaked dwarf virus infection rice by quantitative Real-time PCR

“…6a and b). The result was similar with previous studies [38,39]. Meanwhile, the expression levels of OsWRKY were progressively increased in the early stage of infection and then decreased ( Fig.…”

“…To further investigate the suitability of the selected reference genes, the expression levels of two virus resistant response genes, the gene expression of OsPR1b and OsWRKY, was compared by using single and multiple reference gene(s) for normalization. In the earlier studies [38,39], for example, the expression of OsPR1b gene was strongly induced by virus infection. Our results further showed that when using TIP41-Like gene for normalization, the induction patterns of OsPR1b and OsWRKY transcripts were not stronger than those using two reference genes (Fig.…”

Spatiotemporal Changes in Xylan-1/Xyloglucan and Xyloglucan Xyloglucosyl Transferase (XTH-Xet5) as a Step-In of Ultrastructural Cell Wall Remodelling in Potato–Potato Virus Y (PVYNTN) Hypersensitive and Susceptible Reaction

“…These analyses revealed the effects of plant–virus interactions [14,21], especially about the reinforcing of the cell wall structure. Our knowledge of the mechanisms of resistance to plant pathogens was extended by RNAseq analyses of the whole transcriptomes [14].…”

The receptor-like kinase SlSOBIR1 is differentially modulated by virus infection but its overexpression in tobacco has no significant impact on virus accumulation

“…Although the transcriptional modulation of LRR-RLK genes in response to virus infection has been reported for a number of plantviral pathosystems (Naqvi et al 2011;Mandadi and Scholthof 2012;Chen et al 2013;Czosnek et al 2013;Zheng et al 2013;Andolfo et al 2014), their possible role in antiviral defense remains speculative. These genes are induced by virus infection in most cases, but repression has In this study, we focused on SlSOBIR1, a tomato LRR-RLK implicated in plant immunity and described to be a crucial interactor for RLP-mediated resistance against fungal pathogens (Liebrand et al 2013a, b).…”