Comparative transcriptome analysis of arsenate and arsenite stresses in rice seedlings

Chakrabarty, Debasis; Trivedi, Prabodh Kumar; Misra, Prashant; Tiwari, Manish; Shri, Manju; Shukla, Devesh; Kumar, Smita; Rai, Ankita; Pandey, Ashutosh; Nigam, Deepti; Tripathi, Rudra Deo; Tuli, Rakesh

doi:10.1016/j.chemosphere.2008.09.082

Cited by 263 publications

(193 citation statements)

References 29 publications

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“…The toxic effect of As is highly dependent on its chemical speciation. Arsenite is generally believed to be more toxic to organisms than As(V) (1,5). Moreover, conversion of As(V) to As (III) can generate reactive oxygen species (ROS) and result in damage to DNA, proteins, and lipids (2).…”

Section: Discussionmentioning

confidence: 99%

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Arsenic Accumulation and Speciation in Maize as Affected by Inoculation with Arbuscular Mycorrhizal Fungus Glomus mosseae

Zhang

Huang

et al. 2009

J. Agric. Food Chem.

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Effects of inoculation with arbuscular mycorrhizal (AM) fungus (Glomus mosseae) on arsenic (As) accumulation and speciation in maize were investigated by using As spiked soil at the application levels of 0, 25, 50, and 100 mg kg -1. Inorganic As was the major species in plants, and mycorrhizal inoculation generally decreased concentrations of arsenite [As(III)] in maize roots and concentrations of As(III) and arsenate [As(V)] in the shoots. Dimethylarsenic acid (DMA) concentrations (detected in every plant sample) were higher in maize shoots for mycorrhizal than for nonmycorrhizal treatment, but no significant differences were observed for roots. Monomethylarsenic acid (MMA) was only detected in roots with mycorrhizal colonization. The uptake of As(V) was much lower by excised mycorrhizal than nonmycorrhizal roots, and the differences for the uptake of As(III) were negligible. Arsenate reductase (AR) activity was detected in maize roots, and it was reduced with mycorrhizal inoculation. Activities of peroxidase (POD) and superoxide dismutase (SOD) were detected in both maize shoots and roots, and they were suppressed by mycorrhizal inoculation. AM inoculation inhibited the uptake of As(V) and its reduction to As(III), reducing oxidation stress and thereby alleviating As toxicity to the host plant.

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Section: Discussionmentioning

confidence: 99%

“…Toxic effects of As are highly dependent on its speciation. Inorganic As [As(III) and As(V)] is generally more toxic than the organic species, and As(III) is more toxic than As(V) (1,2,5).…”

Section: Introductionmentioning

confidence: 99%

Arsenic Accumulation and Speciation in Maize as Affected by Inoculation with Arbuscular Mycorrhizal Fungus Glomus mosseae

Zhang

Huang

et al. 2009

J. Agric. Food Chem.

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“…In the microarray analysis, among the 17 genes up-regulated by at least 3-fold by overexpression of MYBS3 as well as by cold in the wild type (Supplemental Fig. S4A), several of them have previously been implicated in stress responses and/or tolerance in plants (Supplemental Table S1), such as Glu decarboxylase, which catalyzes the conversion of Glu to g-aminobutyrate and is activated in response to heat in Arabidopsis roots (Bouche et al, 2004) and to anoxia in rice roots (Aurisano et al, 1995); WRKY77, which activates the ABA-inducible HVA22 promoter in cereal grains (Xie et al, 2005), and several WRKYs have been shown to confer biotic and abiotic stress tolerance in Arabidopsis (Ross et al, 2007;Lai et al, 2008;Zhou et al, 2008); and multidrug resistance protein 4, whose expression is activated by arsenate and arsenite stresses in rice seedlings (Chakrabarty et al, 2009) and whose homologous genes confer salt tolerance (Lee et al, 2004) and oxidative stress tolerance against pathogens (Sun et al, 2006) in various plant species.…”

Section: Mybs3 Suppresses the Dreb1-dependent Pathway Under Prolongedmentioning

confidence: 99%

A Novel MYBS3-Dependent Pathway Confers Cold Tolerance in Rice

et al. 2010

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Rice (Oryza sativa) seedlings are particularly sensitive to chilling in early spring in temperate and subtropical zones and in high-elevation areas. Improvement of chilling tolerance in rice may significantly increase rice production. MYBS3 is a single DNA-binding repeat MYB transcription factor previously shown to mediate sugar signaling in rice. In this study, we observed that MYBS3 also plays a critical role in cold adaptation in rice. Gain-and loss-of-function analyses indicated that MYBS3 was sufficient and necessary for enhancing cold tolerance in rice. Transgenic rice constitutively overexpressing MYBS3 tolerated 4°C for at least 1 week and exhibited no yield penalty in normal field conditions. Transcription profiling of transgenic rice overexpressing or underexpressing MYBS3 led to the identification of many genes in the MYBS3-mediated cold signaling pathway. Several genes activated by MYBS3 as well as inducible by cold have previously been implicated in various abiotic stress responses and/or tolerance in rice and other plant species. Surprisingly, MYBS3 repressed the well-known DREB1/CBFdependent cold signaling pathway in rice, and the repression appears to act at the transcriptional level. DREB1 responded quickly and transiently while MYBS3 responded slowly to cold stress, which suggests that distinct pathways act sequentially and complementarily for adapting short-and long-term cold stress in rice. Our studies thus reveal a hitherto undiscovered novel pathway that controls cold adaptation in rice.

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“…Son zamanlarda, gen ekspresyonundaki ağır metal teşvikli değişimler, mikroçip analizleri ile mRNA seviyesinde araştırılmış ve bitkilerde ağır metal cevaplarının anlaşılması için değerli bilgiler sağlamıştır (Chakrabarty et al 2009;Yu et al 2012;Shen et al 2013). Bununla birlikte, bitkilerde ağır metal stresine cevap olarak eksprese olan proteinler ile ilgili birçok soru cevapsız kalmaktadır.…”

Section: Introductionunclassified

Heavy Metal Toxicity in Plants: Proteomics Approach

Terzi¹,

Yıldız²

2013

AKU-J. Sci. Eng.

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ÖzetAğır metal kirliliği, insan sağlığını olumsuz etkileyen ve tarımsal verimde kayıplara neden olan önemli çevresel tehlikelerden biridir. Bitki hücresinin en önemli fonksiyonu, kendi savunması için ağır metal stresine karşı cevap vermektir. Ağır metal stresine cevap olarak fonksiyonel genler veya proteinlerin teşhisi stres cevaplarının moleküler mekanizmalarının anlaşılmasında önemli bir basamaktır. Protein ekspresyonu sadece transkripsiyonel seviyede değil, aynı zamanda translasyonel ve post-translasyonel seviyelerde de düzenlenmektedir. Bu nedenle, farklı stres koşulları altında bitki proteomundaki değişimleri araştırmak önemlidir. Genom tarafından eksprese olan proteinlerin geniş ölçekli analizi olarak tanımlanan proteomik, sadece proteomun tamamını tanımlamak için değil, aynı zamanda ağır metal stresi gibi farklı fizyolojik koşullar altındaki proteomların karşılaştırılması için güçlü bir moleküler araçtır. Bu derlemede, proteomik teknolojileri ve bitkilerde farklı metabolik olaylarda rolü olan ağır metal teşvikli proteinler tartışılmıştır. Heavy Metal Toxicity in Plants: Proteomics Approach Key wordsPlants; Heavy Metal; Toxicity; Proteomics; Mass Spectrometry AbstractHeavy metal contamination is one of the major environmental hazards, leading to losses in agricultural yields and harmfully affecting human health. The most crucial function of plant cell is to respond against heavy metal stress for self-defence. The identification of the functional genes or proteins that are involved in responses to heavy-metal stress is a fundamental step in understanding the molecular mechanisms of stress responses. Protein expression is regulated not only at the transcriptional level, but also at the translational and post-translational levels. Therefore, it is crucial to investigate the changes in plant proteome under different stress conditions. Proteomics, defined as the large-scale analysis of proteins expressed by the genome, is not only a powerful molecular tool for describing complete proteome, but also for comparative proteomes under different physiological conditions, such as heavy metal stress. In this review, proteomics technologies, and heavy metal induced proteins which play a role in different metabolic events in plants are discussed.

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Comparative transcriptome analysis of arsenate and arsenite stresses in rice seedlings

Cited by 263 publications

References 29 publications

Arsenic Accumulation and Speciation in Maize as Affected by Inoculation with Arbuscular Mycorrhizal Fungus Glomus mosseae

Arsenic Accumulation and Speciation in Maize as Affected by Inoculation with Arbuscular Mycorrhizal Fungus Glomus mosseae

A Novel MYBS3-Dependent Pathway Confers Cold Tolerance in Rice

Heavy Metal Toxicity in Plants: Proteomics Approach

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