Comparative study on the interaction between transferrin and flavonols: Experimental and computational modeling approaches

Inquiry lipaseoring the mechanism of pancreatic lipase inhibition by isovitexin based on multispectral method and enzyme inhibition assay

“…The study of the binding affinity and interaction of isovitexin with PL involved three quenching assays [51]. Phenylalanine (Phe), Tyr, and Trp are the chromophores of PL, with Trp being the main fluorescent component [52]. At T = 298 K, pH = 7.4, the fluorescence intensity decreased as the concentration of isovitexin was increasing (Figure 3a).…”

“…Additionally, the shift in absorption peaks suggested the formation of new complexes among the molecules. component [52]. At T = 298 K, pH = 7.4, the fluorescence intensity decreased as the concentration of isovitexin was increasing (Figure 3a).…”

Experimental and Computational Insights into the Molecular Interactions between Human Transferrin and Apigenin: Implications of Natural Compounds in Targeting Neuroinflammation

“…A study by Du et al investigated the structural effects on the conformational transition of HTF induced by the binding of flavonoids with different numbers and positions of hydroxyl groups . In a recent investigation, researchers delved into understanding the interaction mechanism between transferrin and four closely related flavonolsgalangin, kaempferol, quercetin, and myricetinutilizing a combination of experimental and computational modeling methods . All of the reports signify the importance of iron homeostasis in AD-associated neuroinflammation, thereby revealing the vital role of HTF in AD therapeutics.…”

“…Flavonoids can be categorized into subclasses, including flavonols, flavones, flavanones, anthocyanidins, isoflavones, dihydroflavonols, and chalcones. These classifications are based on factors such as the linkage positions of the B and C rings, as well as the extent of saturation, oxidation, and hydroxylation of the C ring . Apigenin, structurally 4′,5,7-trihydroxyflavone, is a flavonoid that belongs to a class of flavones and is abundantly present in teas and fruits. , It can effectively cross the blood–brain barrier, making it a vital cog in treating CNS disorders, and is also nontoxic even at very high doses.…”

“… 65 In a recent investigation, researchers delved into understanding the interaction mechanism between transferrin and four closely related flavonols—galangin, kaempferol, quercetin, and myricetin—utilizing a combination of experimental and computational modeling methods. 25 All of the reports signify the importance of iron homeostasis in AD-associated neuroinflammation, thereby revealing the vital role of HTF in AD therapeutics. Thus, in our present work, we will be elucidating the binding and interaction mechanism of apigenin with HTF employing a combined in silico and in vitro approaches.…”