Comparative study of zorbax bio series GF 250 and GF 450 and TSK-Gel 3000 SW and SWXL columns in size-exclusion chromatography of proteins

A Review Size-Exclusion Chromatography for the Analysis of Protein Biotherapeutics and Their Aggregates

“…Evaluation of these smaller particles has shown the advantages in resolution as compared to larger particles. [106,110–112] Liu et al [94] demonstrated these effects for the analysis of a reduced antibody: improved resolution and higher efficiencies were observed for the light and heavy chain using columns with smaller particle sizes (Table 1). [94] …”

A Review Size-Exclusion Chromatography for the Analysis of Protein Biotherapeutics and Their Aggregates

“…Evaluation of these smaller particles has shown the advantages in resolution as compared to larger particles. [106,110–112] Liu et al [94] demonstrated these effects for the analysis of a reduced antibody: improved resolution and higher efficiencies were observed for the light and heavy chain using columns with smaller particle sizes (Table 1). [94] …”

Multiple liquid chromatography separations and nanoESI‐ion trap detection of plasma proteins in search of stroke biomarkers: A pilot study

“…The mobile phase used was 50 mM ammonium acetate pH 6.5 with 5% of methanol at a flow rate of 0.7 mL min −1 . The addition of methanol decreased the unspecific interaction of the sample with the stationary phase . The sample was loaded using a 200‐μL loop and injected twice per sample.…”

Efficiency of packed microcolumns compared with large‐bore packed columns in size‐exclusion chromatography

“…The particles are deactivated either by polymer coating or by chemical bonding of a hydrophilic compound to the surface. This combination of features allows SEC to be performed with efficiencies and time scales comparable to those achieved by other LC modes (15).…”

A Review Size-Exclusion Chromatography for the Analysis of Protein Biotherapeutics and Their Aggregates

“…Evaluation of these smaller particles has shown the advantages in resolution as compared to larger particles. [106,110–112] Liu et al [94] demonstrated these effects for the analysis of a reduced antibody: improved resolution and higher efficiencies were observed for the light and heavy chain using columns with smaller particle sizes (Table 1). [94] …”

Multiple liquid chromatography separations and nanoESI‐ion trap detection of plasma proteins in search of stroke biomarkers: A pilot study

“…The mobile phase used was 50 mM ammonium acetate pH 6.5 with 5% of methanol at a flow rate of 0.7 mL min −1 . The addition of methanol decreased the unspecific interaction of the sample with the stationary phase . The sample was loaded using a 200‐μL loop and injected twice per sample.…”

Efficiency of packed microcolumns compared with large‐bore packed columns in size‐exclusion chromatography

“…The particles are deactivated either by polymer coating or by chemical bonding of a hydrophilic compound to the surface. This combination of features allows SEC to be performed with efficiencies and time scales comparable to those achieved by other LC modes (15).…”

A Review Size-Exclusion Chromatography for the Analysis of Protein Biotherapeutics and Their Aggregates

“…Evaluation of these smaller particles has shown the advantages in resolution as compared to larger particles. [106,110–112] Liu et al [94] demonstrated these effects for the analysis of a reduced antibody: improved resolution and higher efficiencies were observed for the light and heavy chain using columns with smaller particle sizes (Table 1). [94] …”

Multiple liquid chromatography separations and nanoESI‐ion trap detection of plasma proteins in search of stroke biomarkers: A pilot study

“…The mobile phase used was 50 mM ammonium acetate pH 6.5 with 5% of methanol at a flow rate of 0.7 mL min −1 . The addition of methanol decreased the unspecific interaction of the sample with the stationary phase . The sample was loaded using a 200‐μL loop and injected twice per sample.…”

Efficiency of packed microcolumns compared with large‐bore packed columns in size‐exclusion chromatography

“…The particles are deactivated either by polymer coating or by chemical bonding of a hydrophilic compound to the surface. This combination of features allows SEC to be performed with efficiencies and time scales comparable to those achieved by other LC modes (15).…”