Comparative study of the effect of hydrocortisone and thyroxine on suckling mouse small intestine in organ culture

Epidermal Growth Factor Does Not Act as a Primary Cue for Inducing Developmental Changes in Suckling Mouse Jejunum

“…Because organ culture removes the studied tissue from the interacting complexities of the intact organism, it permits a more systematic approach to the study of the intrinsic and extrinsic modulators of intestinal maturation than is possible in vivo. This methodology made it possible to confirm the direct influence of glucocorticoid hormones on the maturation of suckling rat (15) and mouse small intestine (16). Furthermore, it has enabled researchers to illustrate that thyroxine was completely ineffective regarding the induction of brush border enzymes as well as DNA synthesis (15,16), thus supporting the hypothesis that thyroxine does not act as a primary cue for inducing developmental changes (17).…”

Epidermal Growth Factor Does Not Act as a Primary Cue for Inducing Developmental Changes in Suckling Mouse Jejunum

“…Because organ culture removes the studied tissue from the interacting complexities of the intact organism, it permits a more systematic approach to the study of the intrinsic and extrinsic modulators of intestinal maturation than is possible in vivo. This methodology made it possible to confirm the direct influence of glucocorticoid hormones on the maturation of suckling rat (15) and mouse small intestine (16). Furthermore, it has enabled researchers to illustrate that thyroxine was completely ineffective regarding the induction of brush border enzymes as well as DNA synthesis (15,16), thus supporting the hypothesis that thyroxine does not act as a primary cue for inducing developmental changes (17).…”

Caco‐2 cells cultured in serum‐free medium as a model for the study of enterocytic differentiation in vitro

“…Most of these studies were performed in vivo, an approach which allows the identification of factors involved in the regulation of enterocyte differentiation but which cannot provide information on the mechanisms underlying their effects. Nevertheless, the organotypic culture of intestinal explants maintained under serum-free conditions has confirmed the direct action of insulin (Moog and Goellner, 1982;Arsenault and Menard, 1984a) and HC (Arsenault and Menard, 198413;Beaulieu and Calvert, 1985) on the intestinal mucosa. Considering the complexity of intestinal tissue itself and the importance of epithelio-mesenchymal interactions for the induction of intestinal maturation (Kedinger et al, 1981) and for the mediation of endodermal cell responses to hormonal stimulation (Lacroix et al, 1989, final proof of the direct effect of hormones on intestinal epithelial cells cannot be assessed definitively under these conditions.…”

Insulin Influences the Maturation and Proliferation of Suckling Mouse Intestinal Mucosa in Serum-Free Organ Culture

“…Insulin injected to adult rats stimu lates the brush border enzymes activities such as lactase and sucrase [ 15] and therefore could be implicated in the development as well as in the maintenance of hydrolytic functions of the intestinal mucosa. The use of organ culture permitted also to demonstrate convincingly the role of glucocorticoids on the induction of sucrase and on the regulation of the levels of the other intestinal brush border enzymes of suckling rats and mice [1,12,29],…”

Epidermal Growth Factor Does Not Act as a Primary Cue for Inducing Developmental Changes in Suckling Mouse Jejunum

“…Because organ culture removes the studied tissue from the interacting complexities of the intact organism, it permits a more systematic approach to the study of the intrinsic and extrinsic modulators of intestinal maturation than is possible in vivo. This methodology made it possible to confirm the direct influence of glucocorticoid hormones on the maturation of suckling rat (15) and mouse small intestine (16). Furthermore, it has enabled researchers to illustrate that thyroxine was completely ineffective regarding the induction of brush border enzymes as well as DNA synthesis (15,16), thus supporting the hypothesis that thyroxine does not act as a primary cue for inducing developmental changes (17).…”

Caco‐2 cells cultured in serum‐free medium as a model for the study of enterocytic differentiation in vitro

“…Most of these studies were performed in vivo, an approach which allows the identification of factors involved in the regulation of enterocyte differentiation but which cannot provide information on the mechanisms underlying their effects. Nevertheless, the organotypic culture of intestinal explants maintained under serum-free conditions has confirmed the direct action of insulin (Moog and Goellner, 1982;Arsenault and Menard, 1984a) and HC (Arsenault and Menard, 198413;Beaulieu and Calvert, 1985) on the intestinal mucosa. Considering the complexity of intestinal tissue itself and the importance of epithelio-mesenchymal interactions for the induction of intestinal maturation (Kedinger et al, 1981) and for the mediation of endodermal cell responses to hormonal stimulation (Lacroix et al, 1989, final proof of the direct effect of hormones on intestinal epithelial cells cannot be assessed definitively under these conditions.…”

Insulin Influences the Maturation and Proliferation of Suckling Mouse Intestinal Mucosa in Serum-Free Organ Culture

“…Insulin injected to adult rats stimu lates the brush border enzymes activities such as lactase and sucrase [ 15] and therefore could be implicated in the development as well as in the maintenance of hydrolytic functions of the intestinal mucosa. The use of organ culture permitted also to demonstrate convincingly the role of glucocorticoids on the induction of sucrase and on the regulation of the levels of the other intestinal brush border enzymes of suckling rats and mice [1,12,29],…”

Epidermal Growth Factor Does Not Act as a Primary Cue for Inducing Developmental Changes in Suckling Mouse Jejunum

“…Because organ culture removes the studied tissue from the interacting complexities of the intact organism, it permits a more systematic approach to the study of the intrinsic and extrinsic modulators of intestinal maturation than is possible in vivo. This methodology made it possible to confirm the direct influence of glucocorticoid hormones on the maturation of suckling rat (15) and mouse small intestine (16). Furthermore, it has enabled researchers to illustrate that thyroxine was completely ineffective regarding the induction of brush border enzymes as well as DNA synthesis (15,16), thus supporting the hypothesis that thyroxine does not act as a primary cue for inducing developmental changes (17).…”

Caco‐2 cells cultured in serum‐free medium as a model for the study of enterocytic differentiation in vitro

“…Most of these studies were performed in vivo, an approach which allows the identification of factors involved in the regulation of enterocyte differentiation but which cannot provide information on the mechanisms underlying their effects. Nevertheless, the organotypic culture of intestinal explants maintained under serum-free conditions has confirmed the direct action of insulin (Moog and Goellner, 1982;Arsenault and Menard, 1984a) and HC (Arsenault and Menard, 198413;Beaulieu and Calvert, 1985) on the intestinal mucosa. Considering the complexity of intestinal tissue itself and the importance of epithelio-mesenchymal interactions for the induction of intestinal maturation (Kedinger et al, 1981) and for the mediation of endodermal cell responses to hormonal stimulation (Lacroix et al, 1989, final proof of the direct effect of hormones on intestinal epithelial cells cannot be assessed definitively under these conditions.…”

Insulin Influences the Maturation and Proliferation of Suckling Mouse Intestinal Mucosa in Serum-Free Organ Culture

“…Insulin injected to adult rats stimu lates the brush border enzymes activities such as lactase and sucrase [ 15] and therefore could be implicated in the development as well as in the maintenance of hydrolytic functions of the intestinal mucosa. The use of organ culture permitted also to demonstrate convincingly the role of glucocorticoids on the induction of sucrase and on the regulation of the levels of the other intestinal brush border enzymes of suckling rats and mice [1,12,29],…”