2018
DOI: 10.1016/j.bjm.2017.04.015
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Abstract: DNA genotyping of Mycobacterium tuberculosis has been widely applied in the understanding of disease transmission in many countries. The purpose of this study was to genotype the strains of M. tuberculosis isolated in patients with new tuberculosis (TB) cases in Minas Gerais, as well as to compare the similarity, discriminatory power, and agreement of the clusters between the IS6110 Restriction Fragment Length Polymorfism (RFLP) and 12 loci Variable Number Tandem Repeat – Mycobacterial Interspersed Repetitive … Show more

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Cited by 9 publications
(5 citation statements)
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References 27 publications
(40 reference statements)
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“…31 reported 3.1% of mixed infections by combining RFLP and spoligotyping in a rural area of Vietnam, equal frequency of our study, using both methods. Another study carried out a retrospective genotyping in previously samples suspected of having epidemiological relationships 32 , which in contrary to the present study, they described a slightly more adequate RFLP cluster results compared to MIRU-VNTR 32 , 33 . Additionally, RFLP lacks the discriminatory power for strains presenting less than six banding patterns, while spoligotyping and MIRU-VNTR are more adequate in this regard.…”
Section: Discussioncontrasting
confidence: 95%
“…31 reported 3.1% of mixed infections by combining RFLP and spoligotyping in a rural area of Vietnam, equal frequency of our study, using both methods. Another study carried out a retrospective genotyping in previously samples suspected of having epidemiological relationships 32 , which in contrary to the present study, they described a slightly more adequate RFLP cluster results compared to MIRU-VNTR 32 , 33 . Additionally, RFLP lacks the discriminatory power for strains presenting less than six banding patterns, while spoligotyping and MIRU-VNTR are more adequate in this regard.…”
Section: Discussioncontrasting
confidence: 95%
“…Furthermore, based on the allelic diversity, there is previous evidence suggesting that the number and combination of loci to be used to discriminate between genotypes can be adapted to the population of Mtb strains [4648]. In this study, applying 12 loci MIRU [49, 50] allowed us to detect the existence of clusters with members of the LAM, H or S families respectively, with the presence of T isolates inserted in between. Interestingly, when evaluating conflicting genotypes, Ag85C SNP and Spotclust classification showed to be useful to solve the issues.…”
Section: Discussionmentioning
confidence: 95%
“…In the case of MIRU-VNTR data, some articles were excluded because they only contained information on M. tuberculosis var. bovis (n = 5); 76 , 77 , 78 , 79 , 80 no data on genotyping were available (n = 6); 49 , 66 , 81 , 82 , 83 , 84 incomplete information on genetic diversity (n = 3) 85 , 86 , 87 or genotyping data is mixed with samples from other countries (n = 3). 84 , 88 , 89 …”
Section: Resultsmentioning
confidence: 99%