Comparative study of non-porous anion-exchange chromatography, capillary gel electrophoresis and capillary electrophoresis in polymer solutions in the separation of DNA restriction fragments

Dynamic Coating Capillary Electrophoresis for Separation of Humic Acid Using Mixture Solution of Non-ionic Polymers both as Coating Agent and Separation Medium

“…The polyacrylamide-coated capillaries were prepared in the same manner as described in an earlier paper. 13 Sample solutions containing 0.5 mg/mL of humic substances were prepared with 10 mM of tris(hydroxymethyl) aminomethane (Tris)/HCl buffer (pH 8.0). All the samples were filtrated by a Millipore (Bedford, MA) Millex syringe-driven filter unit (PTFE membrane, pore size 0.45 μm) before the CE run.…”

“…In the CE separation of basic proteins using the neat electrophoretic buffer solution without polymers and the coated capillary with HEC and PEG by preliminary dynamic coating procedure, though the coated capillary of prepared with the solution containing only PEG 8000000 or PEG 10000 could be used only in lower pH conditions below pH 6, that prepared with the solution containing both PEG 8000000 and PEG 10000 could The polyacrylamide-coated capillaries were prepared in the same manner as described in an earlier paper. 13 be used in higher pH conditions around neutral. 20 Therefore, the addition of PEG of large molecular weight was tested with the expectation that the capillary coating would become firm due to the tangle of polymer chains because it was found that the capillary coating with HEC and PEG 10000 was not stable in weakly alkaline conditions.…”

“…10,11 We previously reported a powerful CE separation technique for humic substances using a polyacrylamide-coated capillary and an electrophoretic buffer solution containing hydroxyethyl cellulose (HEC). 12 In the method, electroosmotic flow (EOF) must be eliminated by immobilizing polyacrylamide on the capillary inner wall via silane coupling treatment followed by in-capillary polymerization 13 to avoid the delay of separation time since humic substances are polyanions with large electrophoretic mobility against the direction of EOF. Though the separation performance of the method is excellent, the procedure for preparing the polyacrylamide-coated capillary is troublesome.…”

High‐Performance Liquid Chromatography of Biological Macromolecules

“…711,712 However, liquid chromatographic techniques have been important in nucleic acid research, often in combination with electrophoretic techniques, 713 and are still widely used for clean-up, sample preparation, and for analytical separations. 714 Polymeric nucleic acids are linear polyanions, where the chains are held together by phosphodiester links between the 3 0 and 5 0 hydroxy groups in the (deoxy)riboses of adjacent nucleotides.…”

Rapid and reproducible capillary electrophoretic separation of double-stranded DNA fragments in a simple methyl cellulosic sieving system

“…In addition to favoring rapid analyses [8], the cellulose derivatives are inexpensive, non-toxic, and easy to work with [8]. Efficiencies of 2-8.3 million, 0.4-8.5 million, and 0.8-4 million plates/m have been reported in PAA gel [9,10], LPAA [11][12][13][14], and cellulose derivative solutions [15,16], respectively. Of the cellulose derivatives, hydroxyethyl cellulose has been the most investigated.…”