Comparative study of DDT and its derivatives in human blood samples in norfolk county and Holland Marsh, Ontario

Brown, John Russell; Chow, Linda

doi:10.1007/bf01721856

Cited by 17 publications

(4 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Serum levels of DDT and its metabolites can be used to determine body burden (Davies et al, 1969;Radomski et al, 1971;Brown and Chow, 1975). However, few studies have established the levels of DDT in the sera of populations protected by this insecticide against malaria.…”

Section: Introductionmentioning

confidence: 99%

Malaria control and levels of DDT in serum of two populations in KwaZulu

Bouwman

Cooppan²,

Becker³

et al. 1991

Journal of Toxicology and Environmental Health

View full text Add to dashboard Cite

Concentrations of p,p'-DDT, p,p'-DDE, and p,p'-DDD were determined in serum of members of households of two different areas of KwaZulu. Annual intradomiciliary application of DDT is used for the interruption of malaria transmission in one area (the exposed group) while the other served as the control. Demographic differences between the two groups resulted in significantly more females in the control group. The two groups were comparable with respect to age. Serum from household members living in DDT-treated dwellings had significantly higher (p less than .005) levels of sigma DDT and metabolites (mean sigma DDT 140.9 micrograms/l) than those from the control area (mean sigma DDT 6.04 micrograms/l). Percentage DDT was also significantly higher (p less than .05) in the exposed group (28.9%) than the control group (8.3%). sigma DDT for the 3-10 yr age interval (168.6 micrograms/l) was significantly higher (p less than .05) than the 20-29 (60.5 micrograms/l) and 30-39 (84.2 micrograms/l) yr age intervals. There seemed to be two groups with regard to accumulation and elimination. The age group 3-29 appeared to be eliminating DDT, most likely accumulated from contaminated breast milk, faster than they accumulated it. From around 29 yr of age accumulation predominated as the levels increased with age. Regression analysis suggested pharmacokinetic differences for DDE and DDT between the two groups. Liver function parameters between the two groups only differed significantly for gamma-glutamyl transferase (gamma GT) (p less than .005), but the influence of difference in alcohol consumption, which was significantly higher in the exposed group (p less than .0001), offered a better explanation. Those of the exposed group that consumed alcohol had a significantly higher (p less than .05) mean gamma GT level (41.5 IU/l) than those that did not (20.2 IU/l), but were not significantly different for sigma DDT (p greater than .05). The safety of DDT used in malaria control for subjects aged 3 and older was confirmed by the levels of DDT in serum when compared with other studies, which showed lack of any negative effects associated with these levels in adults, and an apparently normal liver function in the exposed and control groups.

show abstract

Section: Introductionmentioning

confidence: 99%

Malaria control and levels of DDT in serum of two populations in KwaZulu

Bouwman

Cooppan²,

Becker³

et al. 1991

Journal of Toxicology and Environmental Health

View full text Add to dashboard Cite

show abstract

“…In the last ten years, we have learned that these chemicals accumulate greatly in food chains where concentrations greater than 70,000 times ambient levels have been recorded. They can cause reproductive, developmental and metabolic irregularities, as well as death, in animals and man (Brown and Chow 1975).…”

Section: Additional Trends In Wildlife In Relation To Selected Chemicalsmentioning

confidence: 99%

Biological monitoring and selected trends in environmental quality. [Use of National Inventory of Selected Biological Monitoring Programs at ORNL to identify documented environmental trends]

Suffern¹,

West²,

Kemp³

et al. 1976

View full text Add to dashboard Cite

Th is report was prepared as an account of work sponsored by the Un1ted Stat es Government. Neither the United States nor the Energy Research and Development Administration / United States Nuclear Regulatory Commission , nor any of the1r employees , nor any of their contractors. subcontractors , or their employees. mak es any warranty , express or implied, or assumes any legal liability or respon sibil i ty for the accuracy, completeness or usefulness of any information, apparatus, product or process disclosed, or represents that its use would not infringe privately owned nghts .

show abstract

“…5,6 Because there is a definite relationship between the amount of DDT and its residues in blood and those present in human fat depot, blood can be used easily for assessing the total body burden of persistent pesticides in various populations. 7 Here, an attempt has been made to develop a simple and sensitive bioassay by radiolabelling of the 5 broken ends to estimate the DNA breaks. This method was applied for the evaluation of possible DNA damage among pesticide workers by using DNA extracted from their blood, and the results obtained are presented here.…”

Section: Introductionmentioning

confidence: 99%

Simple radioactive assay for the estimation of DNA breaks

Nair¹,

Paulmurugan

Singh³

2002

J of Applied Toxicology

View full text Add to dashboard Cite

The intactness of DNA is an important part of the normal cellular structure. Any change to the DNA in the form of breaks leads to a change in the integrity, which in turn leads to abnormality in the cellular activity. Many discrepancies have been reported among the various methods of detecting DNA damage. Here, a simple, sensitive and reproducible method has been developed for the detection of DNA breaks by radioactive labelling of 5' broken ends. The method was evaluated by studying chemically induced DNA damage by using both organochloride (2,4-dichlorophenoxyacetic acid and lindane) and organophosphorus (sevin and phosphamidon) compounds at different concentrations. Phosphamidon, one of the organophosphorus compounds studied, showed complete degradation of the DNA after treatment. Radioactive analysis of phosphamidon showed higher counts at the lowest concentration (20 microg) of the chemical when compared with the control (2752 scintillation counts per minute, scm). Studies on the chemically induced DNA breaks by radiolabelling revealed that the cumulative effect of the organophosphorus and organochloride compounds showed maximum counts in all the samples (the highest being 2904 scm) when compared with the organophosphorus and organochloride compounds studied separately (the highest being 1881 and 2260 scm, respectively). Radiolabelling studies on the blood samples of 23 pesticide workers by the newly developed assay showed a significant positive correlation (0.893) between the number of years of exposure and the scintillation counts. A maximum of 11,702 scm (for 18 years of exposure) and a minimum of 1682 scm (for 4 years of exposure) were recorded compared with 1253 scm for the negative control. This method can be used effectively for estimation of the DNA breaks, irrespective of its nature.

show abstract

Comparative study of DDT and its derivatives in human blood samples in norfolk county and Holland Marsh, Ontario

Cited by 17 publications

References 6 publications

Malaria control and levels of DDT in serum of two populations in KwaZulu

Malaria control and levels of DDT in serum of two populations in KwaZulu

Biological monitoring and selected trends in environmental quality. [Use of National Inventory of Selected Biological Monitoring Programs at ORNL to identify documented environmental trends]

Simple radioactive assay for the estimation of DNA breaks

Contact Info

Product

Resources

About