Comparative study of certain substituted ions in hydroxyapatite: characterization and effect on cell viability

Korowash, Sara Ibrahim; Abo-Almaged, H.H.; Ibrahim, D. M.

doi:10.1088/2043-6262/ac70d6

Cited by 1 publication

(5 citation statements)

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“…This substitution is most likely the result of an ion exchange with atmospheric CO 2 during the dissolving, stirring, and reaction phases. 33,36 More research is necessary to determine the source of CO 2 and the specific process of replacement because these issues fall outside the purview of the current work. However, the substitution of carbonate into the HA crystal structure causes a decrease in crystallinity and an increase in solubility.…”

Section: Resultsmentioning

confidence: 99%

“…Stoichiometric HA nanopowders were prepared through an aqueous precipitation method. 33 , 34 As raw materials, calcium carbonate (CaCO 3 , ≥99%) and di-ammonium hydrogen phosphate ((NH 4 ) 2 HPO 4 , ≥99%), purchased from Merck KGaA, Germany, were used as a source of Ca 2+ and PO 4 3- ions respectively, with addition of nitric acid. The molar ratio of Ca/P for the reaction was 1.67 and pH was kept at 8.3 by adding ammonia solution.…”

Section: Methodsmentioning

confidence: 99%

“…The molar ratio of Ca/P for the reaction was 1.67 and pH was kept at 8.3 by adding ammonia solution. 33 Sodium selenite (Na 2 SeO 3 , 99%) and/or copper sulfate pentahydrate (CuSO 4 .5H 2 O, ≥98%), purchased from Merck KGaA, Germany, were added during synthesis to form three different hydroxyapatite phases: selenium substituted hydroxyapatite (SeHA) with selenium percentage (1.96 mol% Se/(P+Se)) at the expense of phosphate salt; copper substituted hydroxyapatite (CuHA) with copper percentage (5 mol% Cu/(Ca+Cu)) at the expense of calcium salt; selenium and copper substituted hydroxyapatite (SeCuHA) with selenium percentage (1.96 mol% Se/(P+Se)) and copper percentage (5 mol% Cu/(Ca+Cu)) at the expense of phosphate salt and calcium salt, respectively, as shown in Table 1 . The reaction took place at room temperature then left for 24 h under continuous stirring at 40°C.…”

Section: Methodsmentioning

confidence: 99%

“…Following that, a freeze-drying method was used to produce finer material. 33 The objective of the present work; is to enhance the biocompatibility and antimicrobial properties of synthetic HA by simultaneous ion doping to produce an antimicrobial bioactive material. This material could have a wide range of applications, including toothpaste formulation and use as a filler or in tissue engineering scaffolds to prevent postimplant infections and promote bone/tooth healing.…”

Section: -mentioning

confidence: 99%

Section: Preparation Of Hydroxyapatite Powdersmentioning

confidence: 99%

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Selenium- and/or copper-substituted hydroxyapatite: A bioceramic substrate for biomedical applications

Korowash,

Keskin-Erdogan,

Hemdan

et al. 2023

J Biomater Appl

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Atomic substitution or doping of a bioceramic material hydroxyapatite (HA) with specific ions is an appealing approach for improving its biocompatibility and activity, as well as imparting antibacterial properties. In this study, selenium- and/or copper-substituted hydroxyapatite powders were synthesized by an aqueous precipitation method and using the freeze-drying technique. The molar concentrations of constituents were calculated based on the proposed mechanism whereby selenium (Se4+) ions partially substitute phosphorus (P5+) sites, and copper (Cu2+) ions partially substitute (Ca2+) sites in the HA lattice. Dried precipitated samples were characterized using Inductively coupled plasma optical emission spectroscopy (ICP-OES), X-ray diffraction analysis (XRD), Fourier-transform infrared spectroscopy (FTIR) and Field-emission scanning electron microscopy with energy dispersive X-ray spectroscopy (FESEM-EDX). Accordingly, substitution of Se4+ and/or Cu2+ ions took place in the crystal lattice of HA without the formation of any impurities. The presence of sulphur (S2-) ions in the hydroxyapatite was detected by ICP-OES in all samples with copper substituted in the lattice. The cytotoxicity of the powders on osteoblastic (MC3T3-E1) cells was evaluated in vitro. Selenium substituted hydroxyapatite (SeHA), at the concentration (200 μg/mL), demonstrated higher populations of the live cells than that of control (cells without powders), suggesting that selenium may stimulate the proliferation of these cells. In addition, the copper substituted hydroxyapatite (CuHA) and the selenium and copper substituted hydroxyapatite (SeCuHA) at the concentrations (200 and 300 μg/mL) and (200 μg/mL), respectively demonstrated better results than the unsubstituted HA. Antimicrobial activity was assessed using a well-diffusion method against Streptococcus mutans and Candida albicans, and superior results has obtained with SeCuHA samples. Presented findings imply that selenium and/or copper substituted modified hydroxyapatite nanoparticles, may be an attractive antimicrobial and cytocompatible substrate to be considered for use in a range of translational applications.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: -mentioning

confidence: 99%

Section: Preparation Of Hydroxyapatite Powdersmentioning

confidence: 99%

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