Comparative Studies of Monkey and Human Measles-Virus Strains

Multiplication of measles virus in cell cultures.

“…Similar findings in human amnion cultures were also reported by Seligman and Rapp (125). This change in cytopathogenesis on passage seems to be of rather general occurrence, since Ruckle-Enders (116) stated that measles strains of human and simian origin showed typical syncytial formation in the early passages in human or simian cell cultures, and, after 8 to 28 passages in primary cultures or continuous cell lines, they exhibited an alteration of their original cytopathic effect, with spindlecell degeneration.…”

“…Dense, highly ordered arrays of filaments were found near the center of the nuclear inclusion, sometimes of a two-dimensional, nearly orthogonal arrangement. These observations have been confirmed by subsequent workers in infected human amnion cells (6, 116), HeLa cells (116,128), KB cells (87), and dog kidney cells (130,131). Depending on the plane of sectioning, a particulate-like appearance in ordered array or crystal-like group was seen in the inclusion bodies (6,116,128,130).…”

“…Fusion appears to occur not only between infected cells but also between normalappearing and infected cells (4). This is further supported by the finding that, when extracellular virus is inhibited by antibody, spread of infection from cell to cell in monolayers still continues, as revealed by immunofluorescence studies (101,116). Toyoshima et al (134) and Karaki 65found giant cells in FL cell cultures as early as 8 to 10 hr after infection with heavy inocula.…”

Electron Microscopy of Measles Virus Replication

“…Electron micrographs of tissues infected with measles virus have shown many fibrous filaments VI~w hich occur in both the cytoplasmic and intranuclear inclusion bodies (2,8,10,(13)(14)(15)(16). In our study, inclusion bodies with filamentous fibers were observed in the cytoplasm of HeLa cells 18 to 20 hr after infection with measles virus.…”

Serologic Survey With the Sera of Monkeys in Regard to Their Natural Infection With Measles Virus

“…MINIA is a virus isolated directly from the primary cultures of , monkey kidney cells. However, no serologic or biologic difference from measles virus of human origin was demonstrated, and this virus is considered to have come from monkeys spontaneously infected with measles virus of human origin (Ruckle, 1958;Ruckle-Enders, 1962). Since the monkeys are imported from countries in southeastern Asia where the possibility of infection with rinderpest virus is still remaining among domestic animals and this virus has a close serologic relationship with measles virus (Bogel et al, 1966), it was possible that the monkeys were spontaneously infected with rinderpest virus resulting in an apparent rise of measles antibody.…”