Comparative skin mucus and serum humoral defence mechanisms in the teleost gilthead seabream (Sparus aurata)

Guardiola, Francisco A.; Cuesta, Alberto; Arizcún, Marta; Meseguer, José; Esteban, María Ángeles

doi:10.1016/j.fsi.2014.01.001

Cited by 232 publications

(117 citation statements)

References 75 publications

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“…In the present species, the lysozyme activity level in mucus was consistently higher than that in serum, both in non-challenged and bacteria-challenged groups. This observation is well in agreement with previous reports that lysozyme content in the skin mucus is more abundant than in blood, probably relating to the anti-infection potential of fish mucus as the primary defense [51][52][53][54]. However, under nonstimulated condition, the enzyme activity in the skin is the reverse condition of the expression pattern of lysozyme transcripts in the present species.…”

Section: Discussionsupporting

confidence: 93%

Molecular characterization and expression pattern of c-type and g-type lysozyme isoforms in starry flounder Platichthys stellate infected with Streptococcus parauberis

Kim

Nam

2015

Fish Sci

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Section: Discussionsupporting

confidence: 93%

Molecular characterization and expression pattern of c-type and g-type lysozyme isoforms in starry flounder Platichthys stellate infected with Streptococcus parauberis

Kim

Nam

2015

Fish Sci

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“…The highest overexpression was found in specimens fed Pdp11 probiotic diet with 1.95-fold and 1.74-fold at 15 and 30 days, respectively (Figure 1 B). In previous studies, complement activity was undetected in the skin mucus of seabream [20,50], but it was reported in . At gene level, a c3 up-regulation was found after yeast dietary administration in gilthead specimens [51] whilst bathing of turbot with acid lactic probiotics also up-regulated its transcription in several tissues [52].…”

Section: Effects Of Pdp11 And/or Overcrowding Stress On Immune-relatementioning

confidence: 83%

Differential proteome profile of skin mucus of gilthead seabream (Sparus aurata) after probiotic intake and/or overcrowding stress

Cordero¹,

Morcillo

Cuesta

et al. 2016

Journal of Proteomics

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Differential proteome profile of skin mucus of gilthead seabream (sparus aurata) after probiotic intake and/or overcrowding stress, Journal of Proteomics (2015Proteomics ( ), doi: 10.1016Proteomics ( /j.jprot.2015 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. A C C E P T E DM A N U S C R I P T ACCEPTED MANUSCRIPT A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT2 AbstractGilthead seabream (Sparus aurata L.) is the major cultured fish species in the Mediterranean area. High density stocking causes stress and increases the impact of diseases leading to economic losses. Probiotics could represent a solution to prevent diseases through several mechanisms such as improving the immune status and/or mucosal microbiota or competing with pathogens. The probiotic Shewanella putrefaciens, also known as Pdp11, was firstly isolated from the skin of healthy gilthead seabream. Our study focuses on the skin mucus proteome after dietary probiotic Pdp11 intake in fish maintained under normal or overcrowding conditions. 2-DE of skin mucus followed by LC-MS/MS analysis was done for each experimental group and differentially expressed proteins were identified. The results showed differentially expressed proteins especially involved in immune processes, such as lysozyme, complement C3, natural killer cell enhancing factor and nonspecific cytotoxic cell receptor protein 1, whose transcript profiles were studied by qPCR. A consistency between lysozyme protein levels in the mucus and lysozyme mRNA levels in skin were found. Further research is necessary to unravel the implications of skin mucosal immunity on fish welfare and disease. Biological significanceThe present work reveals the proteomic changes, which are taking place in the skin mucus of stressed and non-stressed gilthead seabream after Pdp11 probiotic intake. The study contributes to improve the knowledge on skin mucosal immunology of this relevant farmed fish species.Furthermore, the paper shows for the first time how a suitable proteomic methodology, in this case 2-DE followed by LC-MS/MS is useful to perform a comparative study with a noninvasive technique of skin mucus of gilthead seabream.

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“…The physico-chemical parameters of skin mucus, such as viscosity, density, conductivity, pH or redox potential, have been described [7]. The presence and abundance of enzymes and/or immunologically active molecules, including proteases, antiproteases, esterase, phosphatase alkaline, peroxidase, lysozyme, immunoglobulin M (IgM) or bacteriostatic peptides, have also been demonstrated in gilthead seabream skin mucus [8,9]. Furthermore, the proteome map of skin mucus in several teleosts [10,11], including gilthead seabream [12,13], has been characterized, as well as the proteomic profiling after dietary supplementation and/or stress [14].…”

Section: Introductionmentioning

confidence: 99%

Dorso-ventral skin characterization of the farmed fish gilthead seabream (Sparus aurata)

et al. 2017

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The skin is the first barrier of defence in fish, protecting against any external stressor and preserving the integrity and homeostasis of the fish body. The aim of this study was to characterise gilthead seabream skin by isolating cells and studying the cell cycle by flow cytometry, to study the skin histology by scanning electron microscopy and the transcription level of some immune-relevant genes by RT-PCR. Furthermore, the results obtained from samples taken from the dorsal and the ventral part of the specimens are compared. No differences were observed in the cell cycle of cells isolated from the dorsal and ventral zones of the skin or in the gene expression of the genes studied in both epidermal zones. However, the epidermis thickness of the ventral skin was higher than that of the dorsal skin, as demonstrated by image analysis using light microscopy. Besides, scanning electron microscopy pointed to a greater cell size and area of microridges in the apical part of the dorsal epidermal cells compared with ventral skin epidermal cells. This study represents a step forward in our knowledge of the skin structure of an important farmed teleost, gilthead seabream, one of the most commonly farmed fish worldwide. Furthermore, for functional characterization, experimental wounds were carried out comparing the wound healing rate between the dorsal and ventral regions of skin over the time. The results showed higher ratio of wound healing in the ventral region, whose wounds were closed after 15 days, compared to dorsal region of skin. Taking into account all together, this study represents a step forward in our knowledge of the skin structure and skin regeneration of an important farmed teleost, gilthead seabream, one of the most commonly farmed fish worldwide.

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Comparative skin mucus and serum humoral defence mechanisms in the teleost gilthead seabream (Sparus aurata)

Cited by 232 publications

References 75 publications

Molecular characterization and expression pattern of c-type and g-type lysozyme isoforms in starry flounder Platichthys stellate infected with Streptococcus parauberis

Molecular characterization and expression pattern of c-type and g-type lysozyme isoforms in starry flounder Platichthys stellate infected with Streptococcus parauberis

Differential proteome profile of skin mucus of gilthead seabream (Sparus aurata) after probiotic intake and/or overcrowding stress

Dorso-ventral skin characterization of the farmed fish gilthead seabream (Sparus aurata)

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