Comparative skin histology of neotropical odontophrynid frogs

O’Donohoe, M.E. Ailín; Rosset, Sergio Daniel; Regueira, Eleonora; Haddad, Célio F.B.; Basso, Néstor Guillermo; Hermida, Gladys N.

doi:10.1016/j.jcz.2022.09.004

Cited by 3 publications

(12 citation statements)

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“…27,72,73 The presence of an EK layer was noted in O. americanus specimens from the Mendoza and La Rioja provinces, while it was notably absent in specimens from Coŕdoba and San Luis. 35 In the La Rioja specimen, the EK layer appeared as an interrupted layer, similar to the observations of O. americanus from the Rio Negro province in this study (see Table 2).…”

Section: Study Of Antimicrobial Activity Of Americanin-1: Determinati...supporting

confidence: 88%

“…Based on their morphology, secretory content, and observed histochemical characteristics, two types of OSGs were identified, which was consistent with other species of the same genus. 4,35 We have adopted the same terminology to designate these glands, categorizing them as Type 1 and Type 2. OSG1 exhibits a higher density of granules, testing positive for CooB, negative for PAS, and positive for AB at the periphery (Figures 7B and 7C).…”

Section: Study Of Antimicrobial Activity Of Americanin-1: Determinati...mentioning

confidence: 99%

“…These findings were compared with those reported previously for various specimens of O. americanus collected from the Mendoza, Coŕdoba, San Luis, and La Rioja provinces. 35 Table 3 presents the histochemical reaction results for each type of OSG gland. Note that, in the specimens documented by O'Donohoe et al, both gland types were PAS-negative, whereas our results indicate that OSG1 was PAS-negative, while OSG2 was PAS-positive.…”

Section: Study Of Antimicrobial Activity Of Americanin-1: Determinati...mentioning

confidence: 99%

“…This description offers valuable insights into the integument of Patagonian O. americanus, complementing previously documented descriptions of the O. americanus integument in the Cuyo and Sierra Pampeanas regions of Argentina. 35 ■ RESULTS AND DISCUSSION Identification of Peptides from the Skin of O. americanus. The identification of peptides from the skin of O. americanus involved two distinct strategies.…”

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First Bioprospecting Study of Skin Host-Defense Peptides in Odontophrynus americanus

Cancelarich,

Arrulo,

Gugliotti

et al. 2024

J. Nat. Prod.

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The adaptation of amphibians to diverse environments is closely related to the characteristics of their skin. The complex glandular system of frog skin plays a pivotal role in enabling these animals to thrive in both aquatic and terrestrial habitats and consists of crucial functions such as respiration and water balance as well as serving as a defensive barrier due to the secretion of bioactive compounds. We herein report the first investigation on the skin secretion of Odontophrynus americanus, as a potential source of bioactive peptides and also as an indicator of its evolutionary adaptations to changing environments. Americanin-1 was isolated and identified as a neutral peptide exhibiting moderate antibacterial activity against E. coli. Its amphipathic sequence including 19 amino acids and showing a propensity for α-helix structure is discussed. Comparisons of the histomorphology of the skin of O. americanus with other previously documented species within the same genus revealed distinctive features in the Patagonian specimen, differing from conspecifics from other Argentine provinces. The presence of the Eberth−Katschenko layer, a prevalence of iridophores, and the existence of glycoconjugates in its serous glands suggest that the integument is adapted to retain skin moisture. This adaptation is consistent with the prevailing aridity of its native habitat.

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Section: Study Of Antimicrobial Activity Of Americanin-1: Determinati...supporting

confidence: 88%

Section: Study Of Antimicrobial Activity Of Americanin-1: Determinati...mentioning

confidence: 99%

Section: Study Of Antimicrobial Activity Of Americanin-1: Determinati...mentioning

confidence: 99%

mentioning

confidence: 99%

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First Bioprospecting Study of Skin Host-Defense Peptides in Odontophrynus americanus

Cancelarich,

Arrulo,

Gugliotti

et al. 2024

J. Nat. Prod.

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“…The skin of 6 specimens at late climax was studied for each larval period length. The diameter of syncytial glands (also known as granular or serous glands; see revision of anuran skin glands nomenclature in O'Donohoe et al., 2019, 2022; Regueira et al., 2016) was calculated by following 10–15 individual glands through serially adjacent skin sections. The total number of sections each gland occupied was multiplied by the section thickness of 7 μm.…”

Section: Methodsmentioning

confidence: 99%

Naturally occurring variability in larval period length is associated with intraspecific differences in late tadpoles of a South American true toad

Regueira,

Pavón Novarin,

O'Donohoe

et al. 2023

Journal of Zoology

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The larval period in anurans is highly plastic. Even though larval period length and larval size have been the most studied characteristics showing intraspecific variability, other features such as morphology, behavior, food preferences and physiology have also been described as plastic. Metamorphic changes are triggered by thyroid and interrenal hormones, and environmental signals modulate the onset of metamorphosis via the hypothalamic–pituitary–interrenal axis. The South American bufonid toad Rhinella arenarum from nearby Buenos Aires City has a larval period of 30–120 days under the same environmental conditions. The goal of this study was to evaluate life‐history traits in tadpoles from different localities and potential morphological differences among tadpoles with different larval period length. For this purpose, we reared under natural conditions tadpoles from two different localities nearby Buenos Aires City and in three different years measured their size at the end of metamorphosis and recorded their larval period length. The external appearance, histo‐morphology and morphometry of the skin and kidney/interrenal complex were studied in metamorphs with short and long larval periods (SLP and LLP, respectively). We observed a larval period lasting 30–120 days in tadpoles from different localities or years. SLP metamorphs were smaller, darker, had less protruding eyes without eyelids, a thinner epidermis and fewer skin glands than LLP metamorphs. The secretion of skin glands from SLP tadpoles had acidic glycoconjugates. Kidney size was similar between LLP and SLP metamorphs, but the former had an adult‐like histomorphology. Interrenal gland in LLP early and late climax tadpoles had cells with a high nucleus/cell diameter ratio, a sign of highly active steroidogenic cells. Overall, these results show that intraspecific variability in size, skin and interrenal gland development is associated with differences in larval period length. Distinct skin gland number and secretion, along with distinct interrenal histology at late climax, could have physiological consequences in the post‐metamorphic life.

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